EXGA_ASPFU
ID EXGA_ASPFU Reviewed; 416 AA.
AC Q4WK60;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Probable glucan 1,3-beta-glucosidase A;
DE EC=3.2.1.58;
DE AltName: Full=Exo-1,3-beta-glucanase 1;
DE AltName: Full=Exo-1,3-beta-glucanase A;
DE Flags: Precursor;
GN Name=exgA; Synonyms=exg1; ORFNames=AFUA_1G03600;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
CC -!- FUNCTION: Beta-glucanases participate in the metabolism of beta-glucan,
CC the main structural component of the cell wall. It could also function
CC biosynthetically as a transglycosylase (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Successive hydrolysis of beta-D-glucose units from the non-
CC reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.;
CC EC=3.2.1.58;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000305}.
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DR EMBL; AAHF01000007; EAL88072.1; -; Genomic_DNA.
DR RefSeq; XP_750110.1; XM_745017.1.
DR AlphaFoldDB; Q4WK60; -.
DR SMR; Q4WK60; -.
DR STRING; 746128.CADAFUBP00000394; -.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR EnsemblFungi; EAL88072; EAL88072; AFUA_1G03600.
DR GeneID; 3507984; -.
DR KEGG; afm:AFUA_1G03600; -.
DR VEuPathDB; FungiDB:Afu1g03600; -.
DR eggNOG; ENOG502QPYU; Eukaryota.
DR HOGENOM; CLU_004624_0_1_1; -.
DR InParanoid; Q4WK60; -.
DR OMA; DTHHYQV; -.
DR OrthoDB; 896412at2759; -.
DR Proteomes; UP000002530; Chromosome 1.
DR GO; GO:1990819; C:actin fusion focus; IEA:EnsemblFungi.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0000935; C:division septum; IEA:EnsemblFungi.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0046557; F:glucan endo-1,6-beta-glucosidase activity; IEA:EnsemblFungi.
DR GO; GO:0004338; F:glucan exo-1,3-beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006073; P:cellular glucan metabolic process; IBA:GO_Central.
DR GO; GO:0070879; P:fungal-type cell wall beta-glucan metabolic process; IEA:EnsemblFungi.
DR GO; GO:1904541; P:fungal-type cell wall disassembly involved in conjugation with cellular fusion; IEA:EnsemblFungi.
DR GO; GO:0009251; P:glucan catabolic process; IBA:GO_Central.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Cell wall biogenesis/degradation; Disulfide bond;
KW Glycoprotein; Glycosidase; Hydrolase; Manganese; Metal-binding;
KW Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..416
FT /note="Probable glucan 1,3-beta-glucosidase A"
FT /id="PRO_0000393530"
FT ACT_SITE 211
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 308
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT CARBOHYD 344
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 291..415
FT /evidence="ECO:0000250"
FT DISULFID 316..342
FT /evidence="ECO:0000250"
SQ SEQUENCE 416 AA; 45687 MW; E415DC7AD94260C6 CRC64;
MIFKFSQKAL VALYLVVGLA EAVPSKSRVV SRASTFDYNG IVRGVNIGGW LVLEPWITPS
IFDNAGDAAV DEWTLTATLG QDQAKAVLSQ HWSTFITQDD FQQIAQAGMN HVRIPIGYWA
VSSLPDEPYV DGQLEYLDNA ISWAREAGLK VVIDLHGAPG SQNGFDNSGR KGPIAWQQGD
TVSQTVDAFR ALAERYLPQS DVVTAIEALN EPNIPGGVSE AGLRDYYNQI ADVVRQIDPG
TSVFLSDGFL STESWNGFKT GEDVVMDTHH YEMFDNYLIS LDIDGHVKSA CDFGKQIEGS
DKPVVVGEWS GAVTDCTKHL NGKGVSTRYQ GEYANNVKYG DCANTTQGSV ADLSDQERTD
TRRFIEAQLD AYEGKNGWLF WTWKTEGAPG WDMQDLLANG VFPSPLTDRQ FPNQCA
