EXL1_ARATH
ID EXL1_ARATH Reviewed; 375 AA.
AC Q94CH8; Q84K55; Q9LQS6;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=GDSL esterase/lipase EXL1;
DE EC=3.1.1.-;
DE AltName: Full=Family II extracellular lipase 1;
DE Short=Family II lipase EXL1;
DE Flags: Precursor;
GN Name=EXL1; OrderedLocusNames=At1g75880; ORFNames=T4O12.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=11431566; DOI=10.1126/science.1060972;
RA Mayfield J.A., Fiebig A., Johnstone S.E., Preuss D.;
RT "Gene families from the Arabidopsis thaliana pollen coat proteome.";
RL Science 292:2482-2485(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [6]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q94CH8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q94CH8-2; Sequence=VSP_036681;
CC -!- TISSUE SPECIFICITY: Flower buds. {ECO:0000269|PubMed:11431566}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79814.1; Type=Erroneous gene model prediction; Note=The predicted gene At1g75880 has been split into 2 genes: At1g75880 and At1g75890.; Evidence={ECO:0000305};
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DR EMBL; AY028609; AAK30016.1; -; mRNA.
DR EMBL; AC007396; AAF79814.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35768.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35769.1; -; Genomic_DNA.
DR EMBL; BT004215; AAO42232.1; -; mRNA.
DR EMBL; BT005026; AAO50559.1; -; mRNA.
DR RefSeq; NP_565120.1; NM_106238.4. [Q94CH8-1]
DR RefSeq; NP_974149.1; NM_202420.3. [Q94CH8-2]
DR AlphaFoldDB; Q94CH8; -.
DR SMR; Q94CH8; -.
DR BioGRID; 29140; 6.
DR IntAct; Q94CH8; 6.
DR STRING; 3702.AT1G75880.1; -.
DR PaxDb; Q94CH8; -.
DR PRIDE; Q94CH8; -.
DR ProteomicsDB; 222414; -. [Q94CH8-1]
DR EnsemblPlants; AT1G75880.1; AT1G75880.1; AT1G75880. [Q94CH8-1]
DR EnsemblPlants; AT1G75880.2; AT1G75880.2; AT1G75880. [Q94CH8-2]
DR GeneID; 843921; -.
DR Gramene; AT1G75880.1; AT1G75880.1; AT1G75880. [Q94CH8-1]
DR Gramene; AT1G75880.2; AT1G75880.2; AT1G75880. [Q94CH8-2]
DR KEGG; ath:AT1G75880; -.
DR Araport; AT1G75880; -.
DR TAIR; locus:2204420; AT1G75880.
DR eggNOG; ENOG502QW19; Eukaryota.
DR HOGENOM; CLU_015101_0_1_1; -.
DR InParanoid; Q94CH8; -.
DR OMA; NFIIANC; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; Q94CH8; -.
DR BioCyc; ARA:AT1G75880-MON; -.
DR PRO; PR:Q94CH8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94CH8; baseline and differential.
DR Genevisible; Q94CH8; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016298; F:lipase activity; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR008265; Lipase_GDSL_AS.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
DR PROSITE; PS01098; LIPASE_GDSL_SER; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Hydrolase; Lipid degradation;
KW Lipid metabolism; Reference proteome; Secreted; Signal.
FT SIGNAL 1..40
FT /evidence="ECO:0000255"
FT CHAIN 41..375
FT /note="GDSL esterase/lipase EXL1"
FT /id="PRO_0000367328"
FT ACT_SITE 59
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 350
FT /evidence="ECO:0000250"
FT ACT_SITE 353
FT /evidence="ECO:0000250"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 334
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 148
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_036681"
SQ SEQUENCE 375 AA; 41334 MW; 8E097A7DD407C8F3 CRC64;
MERCRSGHHR IISYYLSSSL ILFWCIFVLV LLSTTSTTNA LVKIPKNTTV PAVIVFGDSI
VDAGNNDDMI TEARCDYAPY GIDFDGGVAT GRFSNGKVPG DIVAEELGIK PNIPAYRNPN
LKPEELLTGV TFASGGAGYV PLTTKIAVGG IPLPQQLIYF EEYIEKLKQM VGEKRTKFII
KNSLFVVICG SNDIANDFFT LPPVRLHYTV ASFTALMADN ARSFAQTLYG YGARRILVFG
APPIGCVPSQ RTVAGGPTRD CVARFNDAAK LFNTKLSANI DVLSRTLQDP TIIYIDIYSP
LLDLILNPHQ YGFKVANKGC CGTGLIEVTA LCNNYTASVC PIRSDYVFWD SFHPTEKAYR
IIVAKLLDRY LNRFF
