EXL4_ARATH
ID EXL4_ARATH Reviewed; 343 AA.
AC Q0WUV7; Q93X95; Q9LQS5;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=GDSL esterase/lipase EXL4;
DE EC=3.1.1.-;
DE AltName: Full=Family II extracellular lipase 4;
DE Short=Family II lipase EXL4;
DE Flags: Precursor;
GN Name=EXL4; OrderedLocusNames=At1g75910; ORFNames=T4O12.250;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-32; 95-103; 130-141 AND
RP 314-325, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta; TISSUE=Pollen;
RX PubMed=11431566; DOI=10.1126/science.1060972;
RA Mayfield J.A., Fiebig A., Johnstone S.E., Preuss D.;
RT "Gene families from the Arabidopsis thaliana pollen coat proteome.";
RL Science 292:2482-2485(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [6]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RA Sassa M., Saito H., Nakamura K., Ishiguro S.;
RT "Function of Arabidopsis EXL4 and EXL6 to form pollen coats.";
RL (In) Proceedings of the 19th international conference on Arabidopsis
RL research, abstract#9044, Montreal (2008).
CC -!- FUNCTION: Required for the formation of pollen coats and male
CC fertility. {ECO:0000269|Ref.7}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix, pollen coat {ECO:0000269|PubMed:11431566, ECO:0000269|Ref.7}.
CC Note=Localized in small granules in the tapetal cells.
CC -!- TISSUE SPECIFICITY: Flower buds and pollen.
CC {ECO:0000269|PubMed:11431566}.
CC -!- DEVELOPMENTAL STAGE: Strongly expressed in tapetal cells at the flower
CC developmental stage 10 to middle 12, where the components of pollen
CC coat are synthesized actively. {ECO:0000269|Ref.7}.
CC -!- DISRUPTION PHENOTYPE: Reduced pollen fertility. Pollen grain exhibit a
CC partial formation of coat and impaired water absorption and germination
CC capacities. {ECO:0000269|Ref.7}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79815.1; Type=Erroneous gene model prediction; Note=The predicted gene At1g75900 has been split into 2 genes: At1g75900 and At1g75910.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY028612; AAK30019.1; -; mRNA.
DR EMBL; AC007396; AAF79815.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35773.1; -; Genomic_DNA.
DR EMBL; AK227028; BAE99091.1; -; mRNA.
DR PIR; A96788; A96788.
DR RefSeq; NP_177719.1; NM_106241.3.
DR AlphaFoldDB; Q0WUV7; -.
DR SMR; Q0WUV7; -.
DR STRING; 3702.AT1G75910.1; -.
DR PaxDb; Q0WUV7; -.
DR PRIDE; Q0WUV7; -.
DR ProteomicsDB; 222266; -.
DR EnsemblPlants; AT1G75910.1; AT1G75910.1; AT1G75910.
DR GeneID; 843924; -.
DR Gramene; AT1G75910.1; AT1G75910.1; AT1G75910.
DR KEGG; ath:AT1G75910; -.
DR Araport; AT1G75910; -.
DR TAIR; locus:2204410; AT1G75910.
DR HOGENOM; CLU_015101_0_1_1; -.
DR InParanoid; Q0WUV7; -.
DR OMA; MRANIWP; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; Q0WUV7; -.
DR BioCyc; ARA:AT1G75910-MON; -.
DR PRO; PR:Q0WUV7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q0WUV7; baseline and differential.
DR Genevisible; Q0WUV7; AT.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0070505; C:pollen coat; IEA:UniProtKB-SubCell.
DR GO; GO:0016298; F:lipase activity; ISS:TAIR.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR008265; Lipase_GDSL_AS.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
DR PROSITE; PS01098; LIPASE_GDSL_SER; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Extracellular matrix; Glycoprotein; Hydrolase;
KW Lipid degradation; Lipid metabolism; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..343
FT /note="GDSL esterase/lipase EXL4"
FT /id="PRO_0000367331"
FT ACT_SITE 35
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 318
FT /evidence="ECO:0000250"
FT ACT_SITE 321
FT /evidence="ECO:0000250"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 21..23
FT /note="Missing (in Ref. 1; AAK30019)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 343 AA; 37911 MW; 66FA2E7D1105936C CRC64;
MCSKITLVLT LFSSYFISTD AVNGSFPALL AFGDSILDTG NNNFLLTFMK GNIWPYGRSF
SMRRATGRFG NGRVFSDIVA EGLGIKKILP AYRKLFNSPS DLRTGVCFAS GGAGVDPVTS
KLLRVLTPKD QVNDFKGYIR KLKATAGPSR ASSIVSNAVI LVSQGNNDIG ISYFGTPTAA
FRGLTPNRYT TKLAGWNKQF MKELYDQGAR KFAVMGVIPL GCLPMTRIFL GGFVITCNFF
ANRVAEQYNG KLRSGTKSWG REAGFRGAKF VYVDMYNTLM DVIKNYRRYG FSNEKNGCCC
MITAIIPCPN PDKYVFYDFV HPSEKAYRTI SKKLVQDIKN GLA
