EXLYS_BPDPK
ID EXLYS_BPDPK Reviewed; 2237 AA.
AC Q8SCY1;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 29-SEP-2021, entry version 59.
DE RecName: Full=Peptidoglycan hydrolase gp181 {ECO:0000305};
DE AltName: Full=Gene product 181 {ECO:0000305};
DE Short=Gp181;
OS Pseudomonas phage phiKZ.
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Phikzvirus.
OX NCBI_TaxID=169683 {ECO:0000312|Proteomes:UP000002098};
OH NCBI_TaxID=287; Pseudomonas aeruginosa.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11916376; DOI=10.1006/jmbi.2001.5396;
RA Mesyanzhinov V.V., Robben J., Grymonprez B., Kostyuchenko V.A.,
RA Bourkaltseva M.V., Sykilinda N.N., Krylov V.N., Volckaert G.;
RT "The genome of bacteriophage phiKZ of Pseudomonas aeruginosa.";
RL J. Mol. Biol. 317:1-19(2002).
RN [2]
RP FUNCTION, DOMAIN, SUBCELLULAR LOCATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=18671939; DOI=10.1016/j.bbrc.2008.07.102;
RA Briers Y., Miroshnikov K., Chertkov O., Nekrasov A., Mesyanzhinov V.,
RA Volckaert G., Lavigne R.;
RT "The structural peptidoglycan hydrolase gp181 of bacteriophage phiKZ.";
RL Biochem. Biophys. Res. Commun. 374:747-751(2008).
RN [3]
RP REVIEW.
RX PubMed=22991936; DOI=10.3109/1040841x.2012.723675;
RA Rodriguez-Rubio L., Martinez B., Donovan D.M., Rodriguez A., Garcia P.;
RT "Bacteriophage virion-associated peptidoglycan hydrolases: potential new
RT enzybiotics.";
RL Crit. Rev. Microbiol. 39:427-434(2013).
CC -!- FUNCTION: Exolysin that catalyzes the cleavage of the host
CC peptidoglycans during virus entry. {ECO:0000269|PubMed:18671939}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 6.2. {ECO:0000269|PubMed:18671939};
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:18671939}.
CC -!- DOMAIN: The C-terminus contains the peptidoglycan hydrolase activity.
CC {ECO:0000269|PubMed:18671939}.
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DR EMBL; AF399011; AAL83082.1; -; Genomic_DNA.
DR RefSeq; NP_803747.1; NC_004629.1.
DR SMR; Q8SCY1; -.
DR CAZy; GH23; Glycoside Hydrolase Family 23.
DR PRIDE; Q8SCY1; -.
DR GeneID; 1258330; -.
DR KEGG; vg:1258330; -.
DR Proteomes; UP000002098; Genome.
DR GO; GO:0003824; F:catalytic activity; IEA:UniProtKB-KW.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0098932; P:disruption by virus of host cell wall peptidoglycan during virus entry; IEA:UniProtKB-KW.
DR GO; GO:0044409; P:entry into host; IDA:UniProtKB.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR008258; Transglycosylase_SLT_dom_1.
DR Pfam; PF01464; SLT; 1.
DR SUPFAM; SSF53955; SSF53955; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Bacteriolytic enzyme;
KW Degradation of host cell envelope components during virus entry;
KW Degradation of host peptidoglycans during virus entry; Reference proteome;
KW Virion; Virus entry into host cell.
FT CHAIN 1..2237
FT /note="Peptidoglycan hydrolase gp181"
FT /id="PRO_0000432976"
FT REGION 695..726
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1214..1251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1880..2042
FT /note="Peptidoglycan hydrolase activity"
FT REGION 2206..2237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 699..726
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1224..1241
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2206..2222
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2237 AA; 245758 MW; 6CA92E901B372F04 CRC64;
MAKKVTLPKG QTGATGTTLG QAGNILDLSD VDDIFGDTPK AKKGSPVTEF FNGIKQGLFD
SVKPQQALKA FMRSAAPDGF SRMFGVYEDT MSTIRDVKDS VERTSASDLL FLTREAQDLA
VKLKDKVPAS VFDRLNNRLE SQIENYKYAE DSNRNYKEIR RRMEAERDED ELKSAIDQVT
LVQRDLAIKA EQGEVKRFAI GQAERGIRDK VSADRFDWMA KAMGQTVDNL SKLASYNEQV
NYSIQKKGLE IQFRSMLHLR KIAQQTEATM ELLNNGFAAL VRNTGIPDHK KSSMKDLVGF
NAAQRVSSSF VDNAIQTLPN FLGNFGSAVT NNATRFANEN IRNFADAVRA GNMFGADAWE
NRYNIAGQFA GSYLGDWTRN SVIPVLGRMA RPGIERFSNN YLGGRHNQAS YLLDNFPAWT
QEYMNNYQNT YGARGILRDI MAPFIPQFTL QDRLKTGSYQ TIGQDSGFNQ LTQRTIVEAI
PGYLSRLLQE TRMIRTGRDD ITREVFDLST GSFMSVEDSA ANTERRLVSR STVRGVSGAL
TDVLEAFDPN KELSIDARKA LTERLIRDAN MNKRFDPEAY ARAGGYDRSK VSGETIKELT
EYIKRQYNIG ADGRMASTNE NFARRQEIST LFLDVRNFSR DPIKEIERLT NAGKTDQLRE
MGIIITEQGI DRINYPRIWE LMSSEVKYEG WGNDNPFDRY SDTPPSDNGG PEQLSPLGDQ
NNPHFIGPMY QSQTERKMRQ AQEQVIRASK LAQEQANKGY DYAQQKVSLA TDYVRDNVPN
QFSELRRNVN IPASMNFGDL RDQLYGQAGD MYSRAVNYSN QLNGYSDIIN QSIADLYTKA
NTFTPVIKGM DFLNGNLIDI NTGMIVEKIS DITGEIKNQA GITVVTAQEV ATGLYNQRGD
LLTKATDIAS QLRDKAAQIA GDARERLTQG LDNVSDMAKD WYIPGREEAV ILGRDLLAGE
YIDTATNQII TNLKDAKGTI INRAGDVVVT AQELAKGLIR SDGFNLRRNI ADTSNWIQRN
VLGGGSTTQK IFNAMGTVAN KAKDFTIGLG KDILSNRDAY LPGMLKPVLQ KVKLKAGEYY
TTAGNLLKSF DEINGPVLDR DGNIVVDEEQ ISELINSDGS KHTAAKSKGL FRTGLGNLAR
GYANMSMRYW KWLGKKSVDT AKGMAGLGYK LLGSPFKKRF SAFTGKVETQ IDKKALDTTT
DQLLAGIWEE LRNQKPDANK PRRGSWQDLT SRVSDTLNGK NNGDEETTES KGLFGKLGDT
LKNIFGKKKG DEEDEGLLED LGLGGKKGGK WAAARQILGR GALAIGGGAL STAAAYASFG
GTGASTNDKL AGAAIVTSNP IMWALKDFLI KPVLGWRGSQ KFKDDLISYR MMQYGATTTD
QMNKVTELEQ LVSSVATRGG DASFDVRALN ARDIIKIFGY GADDGPAIMR LANWIDFRFK
PIFEAWLKGL SKINRSDVDI SEVDSKVPNE LKGQLIRSVS FPYEGNTPYL VLNNPFGEED
LSIDVASIQM KEKELLNKYS ATEKTSVAPK ATSSSFKEST TDVINDTITT IKSKSTDITN
WFRDSTIGKA IKAVSPVESI RKMVTTTVDT IIPKANASDS LTSLQALRVH AYGMQGLDLA
AVNGLLSIES LVNDKMRVAN GKATYTGDIE ELIKWTGQAF GMVTTSDGPD RVKVVDWLYR
RFLPVFKAFI VTARSVSTSI TLSQIETLTA TQRVQIANAI MGATDDEGIS IWKAPSIFNI
VGDMDSVEDL AKISLDEIKK EAETEVAEAP GKSKSAQIAG KNDAASGRSF ASRIIDNVKS
TFNSATTKVT NWMENTSARV SQVIGRAREG VTDTYYTAKY KLGAGGELTP TGQTYGQLAT
GNGGVWENIP MPQSNKSRDA AQATFKAVSE MTGVPVELLN IFCGIESSFN YNAKAPTSSA
AGWFQFIKST WKGMLAKYGA KFGIPADDEN GSLRFDPRIN ALMGAMFLRD NYEYLENALG
RAPTDVDLYL AHFMGPAGAR KFLTRDQNSI GAEIFPDQAR ANRSIFFKTD GSARTLGEIY
QVMENKVAKF RTGGGKNANS QSLGKPKSTE ELMNDAATAK QKDMATDKEL IGGAADTSIT
DSSNNKIGLG KIMSGMASPL RTNAPSMMLP GAPSSATDVS SGQQPVVDTG AATQATVRAS
QIEEQRKVVT SQDKAMLDIA SEQLSVLKQF HADILNYIKN KAANPSAQTG QEQANTIAPS
QRPGRVVDNR PLPIRLR
