ID   EXLYS_BPPH6             Reviewed;         220 AA.
AC   P07582; Q38459;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   29-SEP-2021, entry version 68.
DE   RecName: Full=Peptidoglycan hydrolase gp5 {ECO:0000305};
DE   AltName: Full=Gene product 5 {ECO:0000305};
DE            Short=Gp5;
GN   Name=P5; Synonyms=P11;
OS   Pseudomonas phage phi6 (Bacteriophage phi-6).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes;
OC   Mindivirales; Cystoviridae; Cystovirus.
OX   NCBI_TaxID=10879;
OH   NCBI_TaxID=319; Pseudomonas savastanoi pv. phaseolicola (Pseudomonas syringae pv. phaseolicola).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3754015; DOI=10.1128/jvi.58.1.142-151.1986;
RA   McGraw T., Mindich L., Frangione B.;
RT   "Nucleotide sequence of the small double-stranded RNA segment of
RT   bacteriophage phi 6: novel mechanism of natural translational control.";
RL   J. Virol. 58:142-151(1986).
RN   [2]
RP   FUNCTION, SUBUNIT, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=1390911; DOI=10.1016/0167-4838(92)90073-m;
RA   Caldentey J., Bamford D.H.;
RT   "The lytic enzyme of the Pseudomonas phage phi 6. Purification and
RT   biochemical characterization.";
RL   Biochim. Biophys. Acta 1159:44-50(1992).
RN   [3]
RP   REVIEW.
RX   PubMed=22991936; DOI=10.3109/1040841x.2012.723675;
RA   Rodriguez-Rubio L., Martinez B., Donovan D.M., Rodriguez A., Garcia P.;
RT   "Bacteriophage virion-associated peptidoglycan hydrolases: potential new
RT   enzybiotics.";
RL   Crit. Rev. Microbiol. 39:427-434(2013).
CC   -!- FUNCTION: Muralytic enzyme exposed to host peptidoglycan layer after
CC       membrane fusion during viral entry. Functions as an exolysin that
CC       cleaves the peptide bridge formed by meso-diaminopimelic acid and D-
CC       alanine. Also lyses the host cell late in infection to release the
CC       virions. {ECO:0000269|PubMed:1390911}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.5. {ECO:0000269|PubMed:1390911};
CC       Temperature dependence:
CC         Inactivated at temperatures above 20 degrees Celsius.
CC         {ECO:0000269|PubMed:1390911};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:1390911}.
CC   -!- SUBCELLULAR LOCATION: Virion. Note=Located between the capsid and the
CC       envelope.
CC   -!- SIMILARITY: Belongs to the peptidase U40 family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Val-9 is the initiator.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA32362.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M12921; AAA32361.1; -; Genomic_RNA.
DR   EMBL; M12921; AAA32362.1; ALT_INIT; Genomic_RNA.
DR   PIR; D23368; YVBPF6.
DR   RefSeq; NP_620343.1; NC_003714.1.
DR   RefSeq; NP_620344.1; NC_003714.1.
DR   SMR; P07582; -.
DR   MEROPS; U40.001; -.
DR   GeneID; 956433; -.
DR   GeneID; 956434; -.
DR   KEGG; vg:956433; -.
DR   KEGG; vg:956434; -.
DR   Proteomes; UP000002610; Genome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0098932; P:disruption by virus of host cell wall peptidoglycan during virus entry; IEA:UniProtKB-KW.
DR   GO; GO:0044409; P:entry into host; IDA:UniProtKB.
DR   Gene3D; 1.10.530.50; -; 1.
DR   InterPro; IPR038288; Gp5_sf.
DR   InterPro; IPR019505; Peptidase_U40.
DR   Pfam; PF10464; Peptidase_U40; 1.
PE   1: Evidence at protein level;
KW   Cytolysis; Degradation of host cell envelope components during virus entry;
KW   Degradation of host peptidoglycans during virus entry;
KW   Host cell lysis by virus; Hydrolase; Reference proteome;
KW   Viral release from host cell; Virion; Virus entry into host cell.
FT   CHAIN           1..220
FT                   /note="Peptidoglycan hydrolase gp5"
FT                   /id="PRO_0000164636"
SQ   SEQUENCE   220 AA;  24149 MW;  28BA74411370823D CRC64;
     MSKDSAFAVQ YSLRALGQKV RADGVVGSET RAALDALPEN QKKAIVELQA LLPKAQSVGN
     NRVRFTTAEV DSAVARISQK IGVPASYYQF LIPIENFVVA GGFETTVSGS FRGLGQFNRQ
     TWDRLRRLGR NLPAFEEGSA QLNASLYAIG FLYLENKRAY EASFKGRVFT HEIAYLYHNQ
     GAPAAEQYLT SGRLVYPKQS EAAVAAVAAA RNQHVKESWA