EXO1_ARATH
ID EXO1_ARATH Reviewed; 735 AA.
AC Q8L6Z7; Q9C7N8;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Exonuclease 1;
DE EC=3.1.-.-;
GN Name=EXO1; OrderedLocusNames=At1g29630; ORFNames=F15D2.37;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Putative 5'->3' double-stranded DNA exonuclease which may
CC also contain a cryptic 3'->5' double-stranded DNA exonuclease activity.
CC May be involved in DNA mismatch repair (MMR) (By similarity).
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium ions per subunit. They probably participate in
CC the reaction catalyzed by the enzyme. May bind an additional third
CC magnesium ion after substrate binding. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8L6Z7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8L6Z7-2; Sequence=VSP_030585;
CC -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. EXO1
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51751.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC068667; AAG51751.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31111.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31112.1; -; Genomic_DNA.
DR EMBL; AY140055; AAM98196.1; -; mRNA.
DR PIR; E86419; E86419.
DR RefSeq; NP_001077624.1; NM_001084155.2. [Q8L6Z7-1]
DR RefSeq; NP_174256.2; NM_102703.5. [Q8L6Z7-2]
DR AlphaFoldDB; Q8L6Z7; -.
DR SMR; Q8L6Z7; -.
DR BioGRID; 25075; 3.
DR STRING; 3702.AT1G29630.2; -.
DR PaxDb; Q8L6Z7; -.
DR PRIDE; Q8L6Z7; -.
DR ProteomicsDB; 222237; -. [Q8L6Z7-1]
DR EnsemblPlants; AT1G29630.1; AT1G29630.1; AT1G29630. [Q8L6Z7-2]
DR EnsemblPlants; AT1G29630.2; AT1G29630.2; AT1G29630. [Q8L6Z7-1]
DR GeneID; 839840; -.
DR Gramene; AT1G29630.1; AT1G29630.1; AT1G29630. [Q8L6Z7-2]
DR Gramene; AT1G29630.2; AT1G29630.2; AT1G29630. [Q8L6Z7-1]
DR KEGG; ath:AT1G29630; -.
DR Araport; AT1G29630; -.
DR TAIR; locus:2013633; AT1G29630.
DR eggNOG; KOG2518; Eukaryota.
DR HOGENOM; CLU_008978_4_0_1; -.
DR InParanoid; Q8L6Z7; -.
DR OrthoDB; 796591at2759; -.
DR PhylomeDB; Q8L6Z7; -.
DR PRO; PR:Q8L6Z7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L6Z7; baseline and differential.
DR Genevisible; Q8L6Z7; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0035312; F:5'-3' exodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0032183; F:SUMO binding; IPI:TAIR.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd09908; H3TH_EXO1; 1.
DR CDD; cd09857; PIN_EXO1; 1.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR032641; Exo1.
DR InterPro; IPR037315; EXO1_H3TH.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR044752; PIN-like_EXO1.
DR InterPro; IPR006086; XPG-I_dom.
DR InterPro; IPR006084; XPG/Rad2.
DR InterPro; IPR019974; XPG_CS.
DR InterPro; IPR006085; XPG_DNA_repair_N.
DR PANTHER; PTHR11081; PTHR11081; 1.
DR PANTHER; PTHR11081:SF8; PTHR11081:SF8; 1.
DR Pfam; PF00867; XPG_I; 1.
DR Pfam; PF00752; XPG_N; 1.
DR PRINTS; PR00853; XPGRADSUPER.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00484; XPGI; 1.
DR SMART; SM00485; XPGN; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR PROSITE; PS00842; XPG_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Endonuclease; Excision nuclease; Exonuclease; Hydrolase; Magnesium;
KW Metal-binding; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..735
FT /note="Exonuclease 1"
FT /id="PRO_0000315620"
FT REGION 1..99
FT /note="N-domain"
FT REGION 138..230
FT /note="I-domain"
FT REGION 391..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..429
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 436..456
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 30
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 78
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 150
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 171
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 173
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 226
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT VAR_SEQ 182..251
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_030585"
FT CONFLICT 377
FT /note="F -> L (in Ref. 3; AAM98196)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 735 AA; 82249 MW; 37ACD5A872BFF116 CRC64;
MGIQGLLPLL KSIMVPIHIK ELEGCIVAVD TYSWLHKGAL SCSRELCKGL PTKRHIQYCM
HRVNLLRHHG VKPIMVFDGG PLPMKLEQEN KRARSRKENL ARALEHEANG NSSAAYECYS
KAVDISPSIA HELIQVLRQE NVDYVVAPYE ADAQMAFLAI TKQVDAIITE DSDLIPFGCL
RIIFKMDKFG HGVEFQASKL PKNKDLSLSG FSSQMLLEMC ILSGCDYLQS LPGMGLKRAH
ALITKFKSYD RVIKHLKYST VSVPPLYEES FKRALLTFKH QRVYDPNAED IIHLCDISDN
LGEDSDFVGP SMPQDIAKGI ALGQLDPFTQ LPFQAESVTP KLAVDDISRP KSFKPETVKK
KLDLPVQKNL LTKYFCFASV EAKRKFKAPR ISPMSLTPTD ESPSIPDDNT PDLDALSSQT
TNESPVYSLG ENPCVSEVAE KRDSPDDDAV ERNHKDLHHK YCEREVDRPK SDSLKVIVRS
KYFKQKQEDK SLKQSIPCLN DCSVIGQRKA VKTVINMSSA SKREESHRAI ATSPCLHHDR
IYNDHEDAKE ASFSAMNEVA ERTINTHKIN HQINEEEQNP SVEIPSAFST PENVIPLSSI
AIDSCHGVAT GKRKLDSDEN LHKENLKSKH MRMDETDTAL NAETPLETDD VEKFGSNISH
IGHYSEIAEK SVERFVSAIS SFKYSGTGSR ASGLRAPLKD IRNTCPSKGL SLKPDISKFG
YASSNRHMVT KSRRM
