ID   EXO1_BPT4               Reviewed;         339 AA.
AC   P04521;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   29-SEP-2021, entry version 88.
DE   RecName: Full=Exonuclease subunit 1;
DE            EC=3.1.11.-;
DE   AltName: Full=Gene product 47;
DE            Short=gp47;
GN   Name=47;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=4018026; DOI=10.1002/j.1460-2075.1985.tb02344.x;
RA   Gram H., Rueger W.;
RT   "Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic
RT   organization as deduced by sequence analysis.";
RL   EMBO J. 4:257-264(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   FUNCTION IN HOST DNA DEGRADATION.
RX   PubMed=4305016; DOI=10.1016/0022-2836(68)90394-x;
RA   Kutter E.M., Wiberg J.S.;
RT   "Degradation of cytosin-containing bacterial and bacteriophage DNA after
RT   infection of Escherichia coli B with bacteriophage T4D wild type and with
RT   mutants defective in genes 46, 47 and 56.";
RL   J. Mol. Biol. 38:395-411(1968).
RN   [4]
RP   FUNCTION IN DNA RECOMBINATION.
RX   PubMed=4886654; DOI=10.1128/jvi.3.2.171-175.1969;
RA   Berger H., Warren A.J., Fry K.E.;
RT   "Variations in genetic recombination due to amber mutations in T4D
RT   bacteriophage.";
RL   J. Virol. 3:171-175(1969).
RN   [5]
RP   FUNCTION.
RX   PubMed=7026800; DOI=10.1128/jvi.40.1.65-77.1981;
RA   Mickelson C., Wiberg J.S.;
RT   "Membrane-associated DNase activity controlled by genes 46 and 47 of
RT   bacteriophage T4D and elevated DNase activity associated with the T4 das
RT   mutation.";
RL   J. Virol. 40:65-77(1981).
CC   -!- FUNCTION: Exonuclease that plays a role in viral genome replication,
CC       DNA recombination, and host DNA degradation.
CC       {ECO:0000269|PubMed:4305016, ECO:0000269|PubMed:4886654,
CC       ECO:0000269|PubMed:7026800}.
CC   -!- SUBUNIT: Consists of two subunits: gp46 and gp47.
CC   -!- SIMILARITY: To phage T5 protein D12 and to yeast RAD52. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X01804; CAA25942.1; -; Genomic_DNA.
DR   EMBL; M10160; AAC05391.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42475.1; -; Genomic_DNA.
DR   PIR; A04297; NCBPX4.
DR   RefSeq; NP_049672.1; NC_000866.4.
DR   SMR; P04521; -.
DR   GeneID; 1258781; -.
DR   KEGG; vg:1258781; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0099015; P:degradation of host chromosome by virus; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
DR   Gene3D; 3.60.21.10; -; 1.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF00149; Metallophos; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
PE   1: Evidence at protein level;
KW   Bacterial host gene expression shutoff by virus;
KW   Degradation of host chromosome by virus; DNA damage; DNA repair;
KW   DNA-binding; Exonuclease; Host gene expression shutoff by virus;
KW   Host-virus interaction; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..339
FT                   /note="Exonuclease subunit 1"
FT                   /id="PRO_0000164935"
SQ   SEQUENCE   339 AA;  39166 MW;  23DF594706691440 CRC64;
     MKILNLGDWH LGVKADDEWI RGIQIDGIKQ AIEYSKKNGI TTWIQYGDIF DVRKAITHKT
     MEFAREIVQT LDDAGITLHT IVGNHDLHYK NVMHPNASTE LLAKYPNVKV YDKPTTVDFD
     GCLIDLIPWM CEENTGEILE HIKTSSASFC VGHWELNGFY FYKGMKSHGL EPDFLKTYKE
     VWSGHFHTIS EAANVRYIGT PWTLTAGDEN DPRGFWMFDT ETERTEFIPN NTTWHRRIHY
     PFKGKIDYKD FTNLSVRVIV TEVDKNLTKF ESELEKVVHS LRVVSKIDNS VESDDSEEVE
     VQSLQTLMEE YINAIPDITD SDREALIQYA NQLYVEATQ