EXO1_DANRE
ID EXO1_DANRE Reviewed; 806 AA.
AC Q803U7;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Exonuclease 1;
DE EC=3.1.-.-;
DE AltName: Full=Exonuclease I;
GN Name=exo1; ORFNames=zgc:55521;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: 5'->3' double-stranded DNA exonuclease which may also contain
CC a cryptic 3'->5' double-stranded DNA exonuclease activity. Also
CC exhibits endonuclease activity against 5'-overhanging flap structures
CC similar to those generated by displacement synthesis when DNA
CC polymerase encounters the 5'-end of a downstream Okazaki fragment.
CC Required for DNA mismatch repair (MMR) (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium ions per subunit. They probably participate in
CC the reaction catalyzed by the enzyme. May bind an additional third
CC magnesium ion after substrate binding. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. EXO1
CC subfamily. {ECO:0000305}.
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DR EMBL; BC044187; AAH44187.1; -; mRNA.
DR RefSeq; NP_998634.1; NM_213469.1.
DR AlphaFoldDB; Q803U7; -.
DR SMR; Q803U7; -.
DR STRING; 7955.ENSDARP00000073846; -.
DR PaxDb; Q803U7; -.
DR GeneID; 406778; -.
DR KEGG; dre:406778; -.
DR CTD; 9156; -.
DR ZFIN; ZDB-GENE-040426-2828; exo1.
DR eggNOG; KOG2518; Eukaryota.
DR InParanoid; Q803U7; -.
DR OrthoDB; 796591at2759; -.
DR PhylomeDB; Q803U7; -.
DR Reactome; R-DRE-5358565; Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
DR Reactome; R-DRE-5685938; HDR through Single Strand Annealing (SSA).
DR Reactome; R-DRE-5685942; HDR through Homologous Recombination (HRR).
DR Reactome; R-DRE-5693568; Resolution of D-loop Structures through Holliday Junction Intermediates.
DR Reactome; R-DRE-5693579; Homologous DNA Pairing and Strand Exchange.
DR Reactome; R-DRE-5693607; Processing of DNA double-strand break ends.
DR Reactome; R-DRE-5693616; Presynaptic phase of homologous DNA pairing and strand exchange.
DR Reactome; R-DRE-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-DRE-69473; G2/M DNA damage checkpoint.
DR PRO; PR:Q803U7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0035312; F:5'-3' exodeoxyribonuclease activity; IBA:GO_Central.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0048256; F:flap endonuclease activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0045145; F:single-stranded DNA 5'-3' exodeoxyribonuclease activity; ISS:UniProtKB.
DR GO; GO:0006310; P:DNA recombination; ISS:UniProtKB.
DR GO; GO:0006298; P:mismatch repair; ISS:UniProtKB.
DR CDD; cd09908; H3TH_EXO1; 1.
DR CDD; cd09857; PIN_EXO1; 1.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR032641; Exo1.
DR InterPro; IPR037315; EXO1_H3TH.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR044752; PIN-like_EXO1.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR006086; XPG-I_dom.
DR InterPro; IPR006084; XPG/Rad2.
DR InterPro; IPR019974; XPG_CS.
DR InterPro; IPR006085; XPG_DNA_repair_N.
DR PANTHER; PTHR11081; PTHR11081; 1.
DR PANTHER; PTHR11081:SF8; PTHR11081:SF8; 1.
DR Pfam; PF00867; XPG_I; 1.
DR Pfam; PF00752; XPG_N; 1.
DR PRINTS; PR00853; XPGRADSUPER.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00484; XPGI; 1.
DR SMART; SM00485; XPGN; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR PROSITE; PS00841; XPG_1; 1.
DR PROSITE; PS00842; XPG_2; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA excision; DNA repair; DNA-binding; Endonuclease;
KW Excision nuclease; Exonuclease; Hydrolase; Magnesium; Metal-binding;
KW Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..806
FT /note="Exonuclease 1"
FT /id="PRO_0000154041"
FT REGION 1..99
FT /note="N-domain"
FT REGION 138..229
FT /note="I-domain"
FT REGION 337..426
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 443..475
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 512..754
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 351..388
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 448..475
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..541
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 556..587
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 599..618
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 627..672
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 30
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 78
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 150
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 171
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 173
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 225
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 806 AA; 88594 MW; B7822D194A0C6AE6 CRC64;
MGIQGLLQFI KDASEPMHVK KYRGQTVAVD TYCWLHKGAF SCAEKLAKGE PTDQYVSYCM
KFVDMLLSFG VKPILVFDGR NLPSKQEVEK SRRERRQANL QKGKQLLREG KITEARECFT
RSVNITPSMA HDVIRAARTR GVDCVVAPYE ADAQLAFLNK SDIAQAVITE DSDLLAFGCK
KVILKMDKQG NGLEIEQCHL GRCKSLGNIF TEEKFRYMCI LSGCDYLQSL YGIGLGKACK
LLRMANNPDI LKVIKKMGQY LKMDISVPEE YIEGFTKANN TFLYQLVFDP LRRKVVPLNP
YPDHINPAAL SYAGTNVGDE KGLQMALGNL DINTMQRIDD FNPDAPQTQP PKAPRSSSWN
DRCDKTATTQ ASIWSQNYEP GCTKSQSPTS PKRPPPTRGK ERIVSVQSLK LPQRESQVKR
PREDTSLSVD DLLEQYTAGV KRHCPETQPT TKPLTNDNKV SKENHCGSTS GPFRPRNRFA
TLLQWRNRSE EGTEEQGTCS RFFCHDESNI AETQEDSKQD SSQSVESQEK HVSQSGGDTS
SLCEEPEREQ DKDEPSSPPA SPSCSSRPAS VGLGVFSWSG TTKELNKSVS HPARDSTERQ
RSSSTPSGLS TLQQFHRNKA RISWAGPGLS LSSSPVEGSE DAGNSPGSPP SQDSAYFSQS
SSISASVENS LVTEDNSDKE KERDSVVSNS PSSSPLDRLK PAVNRTKVSG LSRKGACGQG
KGGKIETSAP ARASGLRRKP SGKKNVNNEN SPGLQATISG LWGAFSFKKD SPKLSATKKG
EPMSPVGENV VMETTQADKE IFIIAE
