ID   EXO2_BPT4               Reviewed;         560 AA.
AC   P04522;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Exonuclease subunit 2;
DE            EC=3.1.11.-;
DE   AltName: Full=Gene product 46;
DE            Short=gp46;
GN   Name=46;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=4018026; DOI=10.1002/j.1460-2075.1985.tb02344.x;
RA   Gram H., Rueger W.;
RT   "Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic
RT   organization as deduced by sequence analysis.";
RL   EMBO J. 4:257-264(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 478-560.
RA   Hsu T., Karam J.;
RL   Submitted (APR-1987) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION IN HOST DNA DEGRADATION.
RX   PubMed=4305016; DOI=10.1016/0022-2836(68)90394-x;
RA   Kutter E.M., Wiberg J.S.;
RT   "Degradation of cytosin-containing bacterial and bacteriophage DNA after
RT   infection of Escherichia coli B with bacteriophage T4D wild type and with
RT   mutants defective in genes 46, 47 and 56.";
RL   J. Mol. Biol. 38:395-411(1968).
RN   [5]
RP   FUNCTION IN DNA RECOMBINATION.
RX   PubMed=4886654; DOI=10.1128/jvi.3.2.171-175.1969;
RA   Berger H., Warren A.J., Fry K.E.;
RT   "Variations in genetic recombination due to amber mutations in T4D
RT   bacteriophage.";
RL   J. Virol. 3:171-175(1969).
RN   [6]
RP   FUNCTION.
RX   PubMed=7026800; DOI=10.1128/jvi.40.1.65-77.1981;
RA   Mickelson C., Wiberg J.S.;
RT   "Membrane-associated DNase activity controlled by genes 46 and 47 of
RT   bacteriophage T4D and elevated DNase activity associated with the T4 das
RT   mutation.";
RL   J. Virol. 40:65-77(1981).
CC   -!- FUNCTION: Exonuclease that plays a role in viral genome replication,
CC       DNA recombination, and host DNA degradation.
CC       {ECO:0000269|PubMed:4305016, ECO:0000269|PubMed:4886654,
CC       ECO:0000269|PubMed:7026800}.
CC   -!- SUBUNIT: Consists of two subunits: gp46 and gp47.
CC   -!- SIMILARITY: To phage T5 protein D13 and to yeast RAD52. {ECO:0000305}.
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DR   EMBL; X01804; CAA25945.1; -; Genomic_DNA.
DR   EMBL; M15080; AAA32516.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42472.1; -; Genomic_DNA.
DR   EMBL; M10160; AAC05392.1; -; Genomic_DNA.
DR   PIR; A04298; NCBPX6.
DR   RefSeq; NP_049669.1; NC_000866.4.
DR   SMR; P04522; -.
DR   GeneID; 1258822; -.
DR   KEGG; vg:1258822; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0099015; P:degradation of host chromosome by virus; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038729; Rad50/SbcC_AAA.
DR   Pfam; PF13476; AAA_23; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Bacterial host gene expression shutoff by virus;
KW   Degradation of host chromosome by virus; DNA damage; DNA repair;
KW   DNA-binding; Exonuclease; Host gene expression shutoff by virus;
KW   Host-virus interaction; Hydrolase; Nuclease; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..560
FT                   /note="Exonuclease subunit 2"
FT                   /id="PRO_0000164937"
FT   BINDING         36..43
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   560 AA;  63613 MW;  D107829431BAB2FC CRC64;
     MKNFKLNRVK YKNIMSVGQN GIDIQLDKVQ KTLITGRNGG GKSTMLEAIT FGLFGKPFRD
     VKKGQLINST NKKELLVELW MEYDEKKYYI KRGQKPNVFE ITVNGTRLNE SASSKDFQAE
     FEQLIGMSYA SFKQIVVLGT AGYTPFMGLS TPARRKLVED LLEVGTLAEM DKLNKALIRE
     LNSQNQVLDV KKDSIIQQIK IYNDNVERQK KLTGDNLTRL QNMYDDLAKE ARTLKSEIEE
     ANERLVNIVL DEDPTDAFNK IGQEAFLIKS KIDSYNKVIN MYHEGGLCPT CLSQLSSGDK
     VVSKIKDKVS ECTHSFEQLS THRDNLKVLV DEYRDNIKTQ QSLANDIRNK KQSLIAAVDK
     AKKVKAAIEK ASSEFIDHAD EIALLQEELD KIVKTKTNLV MEKYHRGILT DMLKDSGIKG
     AIIKKYIPLF NKQINHYLKI MEADYVFTLD EEFNETIKSR GREDFSYASF SEGEKARIDI
     ALLFTWRDIA SIVSGVSIST LILDEVFDGS FDAEGIKGVA NIINSMKNTN VFIISHKDHD
     PQEYGQHLQM KKVGRFTVMV