EXO2_BPT5
ID EXO2_BPT5 Reviewed; 612 AA.
AC P11109; Q5DMH5; Q66LT7;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Probable exonuclease subunit 2 {ECO:0000305};
DE EC=3.1.11.- {ECO:0000305};
DE AltName: Full=D13;
GN Name=D13;
GN ORFNames=T5.128 {ECO:0000312|EMBL:AAS77174.1},
GN T5p126 {ECO:0000312|EMBL:AAU05265.1};
OS Escherichia phage T5 (Enterobacteria phage T5).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX NCBI_TaxID=2695836;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3057441; DOI=10.1093/nar/16.21.10353;
RA Kaliman A.V., Kryukov V.M., Bayev A.A.;
RT "The nucleotide sequence of the region of bacteriophage T5 early genes D10-
RT D15.";
RL Nucleic Acids Res. 16:10353-10354(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT "Bacteriophage T5 complete genome.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX12056.1};
RX PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA Hu S.;
RT "Complete genome sequence of bacteriophage T5.";
RL Virology 332:45-65(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=St0 deletion mutant;
RX PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA Boulanger P.;
RT "Insights into bacteriophage T5 structure from analysis of its
RT morphogenesis genes and protein components.";
RL J. Virol. 88:1162-1174(2014).
RN [5]
RP POSSIBLE FUNCTION.
RX PubMed=2547651; DOI=10.1016/0014-5793(89)80887-7;
RA Blinov V.M., Koonin E.V., Gorbalenya A.E., Kaliman A.V., Kryukov V.M.;
RT "Two early genes of bacteriophage T5 encode proteins containing an NTP-
RT binding sequence motif and probably involved in DNA replication,
RT recombination and repair.";
RL FEBS Lett. 252:47-52(1989).
CC -!- FUNCTION: Possible exonuclease that may play a role in viral genome
CC replication, DNA recombination, and host DNA degradation.
CC {ECO:0000250|UniProtKB:P04522, ECO:0000305|PubMed:2547651}.
CC -!- SUBUNIT: Could consist of two subunits: D13 and D12.
CC {ECO:0000250|UniProtKB:P04522}.
CC -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC {ECO:0000305|PubMed:15661140}.
CC -!- SIMILARITY: To phage T4 protein GP46. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAX12056.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY543070; AAS77174.1; -; Genomic_DNA.
DR EMBL; AY692264; AAU05265.1; -; Genomic_DNA.
DR EMBL; AY587007; AAX12056.1; ALT_INIT; Genomic_DNA.
DR PIR; S01933; WDBPT5.
DR RefSeq; YP_006956.1; NC_005859.1.
DR GeneID; 2777609; -.
DR KEGG; vg:2777609; -.
DR Proteomes; UP000002107; Genome.
DR Proteomes; UP000002141; Genome.
DR Proteomes; UP000002503; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0099015; P:degradation of host chromosome by virus; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
DR GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038729; Rad50/SbcC_AAA.
DR Pfam; PF13476; AAA_23; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Bacterial host gene expression shutoff by virus; Coiled coil;
KW Degradation of host chromosome by virus; DNA replication; Early protein;
KW Exonuclease; Host gene expression shutoff by virus; Host-virus interaction;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome;
KW Viral DNA replication.
FT CHAIN 1..612
FT /note="Probable exonuclease subunit 2"
FT /id="PRO_0000164939"
FT COILED 163..193
FT /evidence="ECO:0000255"
FT COILED 288..459
FT /evidence="ECO:0000255"
FT BINDING 35..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 317
FT /note="N -> D (in Ref. 3; AAX12056 and 4; AAU05265)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 612 AA; 68673 MW; 92E27B652D1DE141 CRC64;
MSKITIKTLK FSNVMSYGKD IVIHFDKNPV TQLIGGNGLG KSTIATVIEE LFYNKNSRGI
KKDALFSWNA PKKEYDMHAY FSKDEDEYEL HKVVKSTAKV TLIKNGEDIS GHTATQTYKM
IEEIMGGDFQ TFTKLIYQSV GSNLDFLKAT DATRKAFLVN LFNQEQYKEM SETIKADRKE
IANTLNNLQG QMAVITKILN GKNNLGTLQE PVEVPEFDEE PLAQELTESK IKAALAKSQE
ANITKLRNLD KAVQVAEQSF EPFKNLPAPT DQNEEISSVT RDLTIVTSRA SEVKKRYQKF
KQEASNTECP TCGTHLNTTA AQKAMDMARV EYDPLFKEKQ SLEAKLEQLK KEQLEYVAYT
RAKDALDKAV VARDEFKNSM SDASFEELNV QILQVQIRQL EQEIADGRSK VAIAKEHNAT
VELANAKYKA KLEQIEKAEA EMTEITSKLD GVSEAVADLD ILIAALKNLV GYKLEHSVKV
FEELINKYLS IMTGGKFALG FELDETKLQV VIFNDGNRTS MENCSTGQQS RINLATLLAI
RMLLTSISKV NINLLFLDEV ISFIDTKGLD TLVELLNEEE SLNSIIVSHG HTHPLAHKIT
VKKDAEGFSY LE
