EXO5_ARATH
ID EXO5_ARATH Reviewed; 413 AA.
AC Q9FKK6; Q67XC1;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Exonuclease V, chloroplastic;
DE Short=Exo V;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN OrderedLocusNames=At5g60370; ORFNames=MUF9.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Single-stranded DNA (ssDNA) bidirectional exonuclease. Has
CC both 5'-3' and 3'-5' exonuclease activities with a strong preference
CC for 5'-ends (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the EXO5 family. {ECO:0000305}.
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DR EMBL; AB011483; BAB08222.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97316.1; -; Genomic_DNA.
DR EMBL; AY072102; AAL59924.1; -; mRNA.
DR EMBL; AY142558; AAN13127.1; -; mRNA.
DR EMBL; AK175444; BAD43207.1; -; mRNA.
DR EMBL; AK176898; BAD44661.1; -; mRNA.
DR RefSeq; NP_200845.1; NM_125430.3.
DR AlphaFoldDB; Q9FKK6; -.
DR SMR; Q9FKK6; -.
DR STRING; 3702.AT5G60370.1; -.
DR PaxDb; Q9FKK6; -.
DR PRIDE; Q9FKK6; -.
DR ProteomicsDB; 222330; -.
DR EnsemblPlants; AT5G60370.1; AT5G60370.1; AT5G60370.
DR GeneID; 836159; -.
DR Gramene; AT5G60370.1; AT5G60370.1; AT5G60370.
DR KEGG; ath:AT5G60370; -.
DR Araport; AT5G60370; -.
DR TAIR; locus:2175093; AT5G60370.
DR eggNOG; KOG4760; Eukaryota.
DR HOGENOM; CLU_013225_0_0_1; -.
DR InParanoid; Q9FKK6; -.
DR OMA; STQNWCE; -.
DR OrthoDB; 1601909at2759; -.
DR PhylomeDB; Q9FKK6; -.
DR PRO; PR:Q9FKK6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKK6; baseline and differential.
DR Genevisible; Q9FKK6; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0045145; F:single-stranded DNA 5'-3' exodeoxyribonuclease activity; IBA:GO_Central.
DR GO; GO:0036297; P:interstrand cross-link repair; IBA:GO_Central.
DR Gene3D; 3.90.320.10; -; 1.
DR InterPro; IPR019190; EXOV.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR PANTHER; PTHR14464; PTHR14464; 1.
DR Pfam; PF09810; Exo5; 3.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Chloroplast; DNA-binding; Exonuclease; Hydrolase; Iron;
KW Iron-sulfur; Magnesium; Metal-binding; Nuclease; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..47
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 48..413
FT /note="Exonuclease V, chloroplastic"
FT /id="PRO_0000421760"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 389..413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 129
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 381
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 384
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 390
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT CONFLICT 311
FT /note="V -> G (in Ref. 4; BAD44661/BAD43207)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 413 AA; 46973 MW; 539FFCFAC08AE045 CRC64;
MAESPSESTS DSLSTTTSTK PAQSGTVSIS SPQSHHVVFP EIPIEIVSEE EMAILDAALA
ASRSILPSVI RSVSPSRITA GGSPKTIRSI TLFSKRKLSA CSDIEESYLH RFRRNQALGV
TDLTGTEWCE KQMENVLCFG RRKVNKAMKV GQARHLQLEE EVVKKVRVKV ESNEDKWALK
LLNSIAGVNQ FLFEGRTREL LLLGFVGGQW IVGIIDELRK AYAEESSDSG PILIDTKTRL
RDTLPAEPQR RNGRLQLMLY KLLWDTIVKE GFPTGPFFDY FSLNRNYVLS QDVRDHIANA
GIKAQTLEEI VRYYENTFQM LPIANNQLLL KYEFQKDQSI IAEIRFNHDH EWVMSKYREV
IEFWRNEREA GYTPEEERWK CRYCQFAKSC PGNPSFESPS TSSPTREAPP LPS
