AH2_PRUSE
ID AH2_PRUSE Reviewed; 15 AA.
AC P29260;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 11-DEC-2019, entry version 46.
DE RecName: Full=Amygdalin beta-glucosidase I';
DE EC=3.2.1.117;
DE AltName: Full=Amygdalin hydrolase isozyme I';
DE Short=AH I';
DE Flags: Fragment;
OS Prunus serotina (Black cherry).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=23207;
RN [1]
RP PROTEIN SEQUENCE.
RC TISSUE=Seed;
RX PubMed=16652959; DOI=10.1104/pp.100.1.282;
RA Li C.P., Swain E., Poulton J.E.;
RT "Prunus serotina amygdalin hydrolase and prunasin hydrolase: purification,
RT N-terminal sequencing, and antibody production.";
RL Plant Physiol. 100:282-290(1992).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-amygdalin + H2O = (R)-prunasin + D-glucose;
CC Xref=Rhea:RHEA:14177, ChEBI:CHEBI:4167, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:17019, ChEBI:CHEBI:17396; EC=3.2.1.117;
CC -!- SUBUNIT: Monomer.
CC -!- DEVELOPMENTAL STAGE: Absent from maturing black cherry fruits until 6
CC weeks after flowering. Then, concomitant with cotyledon development,
CC the level of enzyme increases with specificity for embryonal tissues.
CC -!- PTM: Glycosylated.
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DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR GO; GO:0047668; F:amygdalin beta-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase.
FT CHAIN 1..>15
FT /note="Amygdalin beta-glucosidase I'"
FT /id="PRO_0000064499"
FT NON_TER 15
SQ SEQUENCE 15 AA; 1651 MW; F7CC4FA321E12EC4 CRC64;
AKTDPPIHFA SLXRS