ID   EXO5_CANDC              Reviewed;         624 AA.
AC   B9WLF5;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Exonuclease V, mitochondrial;
DE            Short=Exo V;
DE            EC=3.1.-.-;
DE   AltName: Full=Defects in morphology protein 1;
DE   Flags: Precursor;
GN   Name=EXO5; Synonyms=DEM1; ORFNames=CD36_28710;
OS   Candida dubliniensis (strain CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 /
OS   NRRL Y-17841) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=573826;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841;
RX   PubMed=19745113; DOI=10.1101/gr.097501.109;
RA   Jackson A.P., Gamble J.A., Yeomans T., Moran G.P., Saunders D., Harris D.,
RA   Aslett M., Barrell J.F., Butler G., Citiulo F., Coleman D.C.,
RA   de Groot P.W.J., Goodwin T.J., Quail M.A., McQuillan J., Munro C.A.,
RA   Pain A., Poulter R.T., Rajandream M.A., Renauld H., Spiering M.J.,
RA   Tivey A., Gow N.A.R., Barrell B., Sullivan D.J., Berriman M.;
RT   "Comparative genomics of the fungal pathogens Candida dubliniensis and
RT   Candida albicans.";
RL   Genome Res. 19:2231-2244(2009).
CC   -!- FUNCTION: Single strand DNA specific 5'exonuclease involved in
CC       mitochondrial DNA replication and recombination. Releases dinucleotides
CC       as main products of catalysis. Has the capacity to slide across
CC       5'double-stranded DNA or 5'RNA sequences and resumes cutting two
CC       nucleotides downstream of the double-stranded-to-single-stranded
CC       junction or RNA-to-DNA junction, respectively (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EXO5 family. {ECO:0000305}.
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DR   EMBL; FM992695; CAX39916.1; -; Genomic_DNA.
DR   RefSeq; XP_002421916.1; XM_002421871.1.
DR   AlphaFoldDB; B9WLF5; -.
DR   STRING; 42374.XP_002421916.1; -.
DR   EnsemblFungi; CAX39916; CAX39916; CD36_28710.
DR   GeneID; 8050229; -.
DR   KEGG; cdu:CD36_28710; -.
DR   CGD; CAL0000171417; Cd36_28710.
DR   VEuPathDB; FungiDB:CD36_28710; -.
DR   eggNOG; ENOG502QR0P; Eukaryota.
DR   HOGENOM; CLU_019985_0_0_1; -.
DR   OrthoDB; 1601909at2759; -.
DR   Proteomes; UP000002605; Chromosome R.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045145; F:single-stranded DNA 5'-3' exodeoxyribonuclease activity; IEA:InterPro.
DR   InterPro; IPR019190; EXOV.
DR   PANTHER; PTHR14464; PTHR14464; 1.
DR   Pfam; PF09810; Exo5; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; DNA-binding; Exonuclease; Hydrolase; Iron; Iron-sulfur; Magnesium;
KW   Metal-binding; Mitochondrion; Nuclease; Transit peptide.
FT   TRANSIT         1..61
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           62..624
FT                   /note="Exonuclease V, mitochondrial"
FT                   /id="PRO_0000406685"
FT   REGION          37..80
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         160
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         582
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         585
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         591
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   624 AA;  72071 MW;  66C6A9F2A6CC7D5A CRC64;
     MSRFWHFKKF YFSSCFSKQR MSRRIIKNEL LVPMKVTSKH EQEQPITNDE PRSLLSEDAN
     PSPDVESEPE LESEQSENRE DVHEIIRSMV LGTGTTLPRL SNDSLTGIYN YWKLNPNDDL
     PLYNPTKYTP FEFHSQYNQD RSYISTPRLS VTKLLVFSWC ELRSFYQVYS GSVRLPSTQA
     MTQGTKLHSK LEAEIHPEID TTEIEQFLLS NAMSLGELQK AVPIEEDKVV FYLGEVERLA
     VDWAEMLIER LFSLIMGAEA REILLHGYLN FNNRSFVTNK DEIRDPSSVL VSGIVDYVKL
     QNLTNPSDGT LFDDIHGFVD DNFDQVDNVP LVDLSKFLPE AKQILQHYDF RLTFTDVKTR
     SAPQIPRQES VLEAAKFQTF YYRHFFQLLS RDSRFTYFSL IENAERRGHD VDKPLSILTT
     ICLLRKHYHI LFKDFVRLAN GEPIGFAPFD DSAKSMAYDF VSVFQSSDEF SLANPNHTHF
     FEQLREIDGI EYDSILSPLL KVWKTPPTLR YLAARASQLF GVFEENLGDV TSVEYRLNKT
     SQLLSENVYE YDFSEFQVEV ESASKFWNGE REAVPTEDLS RCSFCEFQSK CMVAGGNTTE
     AVEKKTIGPK IRHFLNECES SSKG