ID   EXO5_PICST              Reviewed;         573 AA.
AC   A3M0E6;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 3.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Exonuclease V, mitochondrial;
DE            Short=Exo V;
DE            EC=3.1.-.-;
DE   AltName: Full=Defects in morphology protein 1;
DE   Flags: Precursor;
GN   Name=EXO5; Synonyms=DEM1; ORFNames=PICST_64329;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Single strand DNA specific 5' exonuclease involved in
CC       mitochondrial DNA replication and recombination. Releases dinucleotides
CC       as main products of catalysis. Has the capacity to slide across
CC       5'double-stranded DNA or 5'RNA sequences and resumes cutting two
CC       nucleotides downstream of the double-stranded-to-single-stranded
CC       junction or RNA-to-DNA junction, respectively (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the EXO5 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABN68512.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000502; ABN68512.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_001386541.2; XM_001386504.1.
DR   AlphaFoldDB; A3M0E6; -.
DR   STRING; 4924.XP_001386541.2; -.
DR   EnsemblFungi; ABN68512; ABN68512; PICST_64329.
DR   GeneID; 4840960; -.
DR   KEGG; pic:PICST_64329; -.
DR   eggNOG; ENOG502QR0P; Eukaryota.
DR   HOGENOM; CLU_019985_0_0_1; -.
DR   InParanoid; A3M0E6; -.
DR   OrthoDB; 1601909at2759; -.
DR   Proteomes; UP000002258; Chromosome 8.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045145; F:single-stranded DNA 5'-3' exodeoxyribonuclease activity; IEA:InterPro.
DR   InterPro; IPR019190; EXOV.
DR   PANTHER; PTHR14464; PTHR14464; 1.
DR   Pfam; PF09810; Exo5; 2.
PE   3: Inferred from homology;
KW   4Fe-4S; DNA-binding; Exonuclease; Hydrolase; Iron; Iron-sulfur; Magnesium;
KW   Metal-binding; Mitochondrion; Nuclease; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..573
FT                   /note="Exonuclease V, mitochondrial"
FT                   /id="PRO_0000285325"
FT   BINDING         90
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         538
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         541
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         547
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   573 AA;  65941 MW;  D5F7E0B1489E24FF CRC64;
     MRAKSILKQF LLPRDKNSSE VGKSDSIELF PNDSLHSLYA NWHLPGSHML PLYNPTNISP
     YEFHSRFNSD QSYKRSPRLS VTKLLTDRWC ELSEYYTIYA GSPQFKVTNA ITQGLERHSE
     LEYELHQPID ISLLMETLSG SISDAISHFQ ATIKDVKDPD LLSELDGNPD AAKLAMEWSD
     HSINRLFSLI TTSEAREVLV HGFLNLENSE FVSSIDELKN SQNGNIDIRK VLVSGIVDHF
     KIENLEDPTD LSLFREIRDY MDYYFDTTIG DKQVIDLTKF LQSVKQFVEE HKSAFSVKTT
     DVKTRSVNRL PSSISVLEAA KFQTFYYRKM FGLLSNEHQR TENNHFAYYS LLENARVRGI
     DVDEPLDIVT LVSILRKNYN LFYLDFVKLA NGEPIGFEPF DSYNKGRENT GFALDSVFGI
     AEKCLLAGKP MQLDLIEKIN SLEDFNYDEI LSPDLIKNWK TAPTLRYLAA RCAQFYELFS
     ELLGDHTAVE YHNGRTSQAF HTSESKYTED VIGEQTRKAS TFWNGKRFPI YTRDLSKCNY
     CDFKPRCMVP NHQLQGDLYR KSLGAKINEF LHS