EXO5_SCHPO
ID EXO5_SCHPO Reviewed; 409 AA.
AC Q9Y7L4;
DT 23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Probable exonuclease V, mitochondrial;
DE Short=Exo V;
DE EC=3.1.-.-;
GN Name=exo5; ORFNames=SPBC685.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Single strand DNA specific 5' exonuclease involved in
CC mitochondrial DNA replication and recombination. Releases dinucleotides
CC as main products of catalysis. Has the capacity to slide across
CC 5'double-stranded DNA or 5'RNA sequences and resumes cutting two
CC nucleotides downstream of the double-stranded-to-single-stranded
CC junction or RNA-to-DNA junction, respectively (By similarity).
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}. Mitochondrion {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the EXO5 family. {ECO:0000305}.
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DR EMBL; CU329671; CAB39359.1; -; Genomic_DNA.
DR PIR; T40633; T40633.
DR RefSeq; NP_596136.1; NM_001022054.2.
DR AlphaFoldDB; Q9Y7L4; -.
DR SMR; Q9Y7L4; -.
DR BioGRID; 277620; 10.
DR STRING; 4896.SPBC685.02.1; -.
DR PaxDb; Q9Y7L4; -.
DR EnsemblFungi; SPBC685.02.1; SPBC685.02.1:pep; SPBC685.02.
DR GeneID; 2541105; -.
DR KEGG; spo:SPBC685.02; -.
DR PomBase; SPBC685.02; exo5.
DR VEuPathDB; FungiDB:SPBC685.02; -.
DR eggNOG; KOG4760; Eukaryota.
DR HOGENOM; CLU_013225_0_2_1; -.
DR InParanoid; Q9Y7L4; -.
DR OMA; STQNWCE; -.
DR PhylomeDB; Q9Y7L4; -.
DR PRO; PR:Q9Y7L4; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005739; C:mitochondrion; ISO:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IC:PomBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0045145; F:single-stranded DNA 5'-3' exodeoxyribonuclease activity; ISO:PomBase.
DR GO; GO:0036297; P:interstrand cross-link repair; IBA:GO_Central.
DR GO; GO:0000002; P:mitochondrial genome maintenance; ISO:PomBase.
DR InterPro; IPR019190; EXOV.
DR PANTHER; PTHR14464; PTHR14464; 1.
DR Pfam; PF09810; Exo5; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Cytoplasm; DNA-binding; Exonuclease; Hydrolase; Iron; Iron-sulfur;
KW Magnesium; Metal-binding; Mitochondrion; Nuclease; Nucleus;
KW Reference proteome.
FT CHAIN 1..409
FT /note="Probable exonuclease V, mitochondrial"
FT /id="PRO_0000116758"
FT BINDING 86
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 386
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 389
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 395
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 409 AA; 48395 MW; 4C3FCCB144B4304E CRC64;
MEEYEDFEME DLSELVSYMD YLETQRLTIS QLDYSLSIPP QLLVSREISK LEDEVCEMLK
ESSLFQLFRK HKGYLNVTDL VLPLWCEVQH EYYLLRRIKK KTPKMERGIK LHQILEYETS
PPSERRVLDR TSKEEPWALR LLRQLEGIML LQKNGITREF PIWGYYKESS IFGIIDEISL
NNPSKSNFNS DIRNYFNFKM YDLSFVDNKT RFSSRKPGAS QILSSKVQLM YYVHLFLNYF
PSLGEKQQSI FRDISPTYSN RLHSQSHWWN MFLSQLSLDG TKDLGPKFLE QSILSIPDIP
EDVFAGHNSL NGLYALVFAS AKKLHLRLTD DNLTIAYRND KTGEVVYKDK FSFSNKLLEA
SYTKAYQFWH NLREPEGVPA EEVYKCRSCE FQKECWWLKK KQYYPLSSP
