ID   EXOA_RHIME              Reviewed;         330 AA.
AC   P33691;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Succinoglycan biosynthesis protein ExoA;
DE            EC=2.4.-.-;
GN   Name=exoA; OrderedLocusNames=RB1082; ORFNames=SMb20957;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA   Glucksmann M.A., Reuber T.L., Walker G.C.;
RT   "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT   by Rhizobium meliloti.";
RL   J. Bacteriol. 175:7033-7044(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RCR2011 / SU47;
RX   PubMed=8246891; DOI=10.1007/bf00284690;
RA   Becker A., Kleickmann A., Keller M., Arnold W., Puehler A.;
RT   "Identification and analysis of the Rhizobium meliloti exoAMONP genes
RT   involved in exopolysaccharide biosynthesis and mapping of promoters located
RT   on the exoHKLAMONP fragment.";
RL   Mol. Gen. Genet. 241:367-379(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Glycosyltransferase required for the synthesis of
CC       succinoglycan (EPS I). Needed for the addition of the second sugar
CC       (glucose). Catalyzes the formation of a beta-1,3 linkage with the
CC       galactose lipid carrier.
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; L20758; AAA16046.1; -; Unassigned_DNA.
DR   EMBL; Z22636; CAA80345.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49482.1; -; Genomic_DNA.
DR   PIR; A49348; A49348.
DR   PIR; B95977; B95977.
DR   PIR; S39956; S39956.
DR   RefSeq; NP_437622.1; NC_003078.1.
DR   RefSeq; WP_004434899.1; NC_003078.1.
DR   AlphaFoldDB; P33691; -.
DR   SMR; P33691; -.
DR   STRING; 266834.SM_b20957; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   PRIDE; P33691; -.
DR   EnsemblBacteria; CAC49482; CAC49482; SM_b20957.
DR   GeneID; 25012725; -.
DR   GeneID; 61601037; -.
DR   KEGG; sme:SM_b20957; -.
DR   PATRIC; fig|266834.11.peg.6010; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_025996_19_0_5; -.
DR   OMA; IMHAEGE; -.
DR   BioCyc; MetaCyc:SM_B20957-MON; -.
DR   UniPathway; UPA00631; -.
DR   PRO; PR:P33691; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Exopolysaccharide synthesis; Glycosyltransferase; Membrane;
KW   Plasmid; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..330
FT                   /note="Succinoglycan biosynthesis protein ExoA"
FT                   /id="PRO_0000059181"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   330 AA;  36168 MW;  6A6A919F152602F1 CRC64;
     MSSDELTSTS SLIVIPCLNE ASHIEALIEK LRPSLTPLNA RVVIADGGST DGTREIARRL
     ATEDPRVLFL DNPKRIQSAA VNRAVAELGA GSDYLIRIDA HGTYPDDYCE RLVEDALATG
     ADSVVVAMQT VGFSTFQKAT AFAQNSKLGN GGSKHRTGAV GHWAEHGHHA LMRIEAFKAV
     GGYDESFSHN EDAELDYRLG KAGYRIWMTD KTSMVYYPRA KLVPLFWQYF GYGRGRAKNF
     LKHRAMPGLR QMLPLAVAPI AFGALLAIVN WMAVVPVGVW AAACLGYGVW MALGQRNPYG
     PLAAVAAMVM HLAWSAGFWR ELLDFRRRVA