ID   EXOB_AZOBR              Reviewed;         348 AA.
AC   Q59083;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=UDP-glucose 4-epimerase;
DE            EC=5.1.3.2;
DE   AltName: Full=Galactowaldenase;
DE   AltName: Full=UDP-galactose 4-epimerase;
GN   Name=exoB;
OS   Azospirillum brasilense.
OG   Plasmid pRhico (90-MDa megaplasmid).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Azospirillaceae; Azospirillum.
OX   NCBI_TaxID=192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29145 / DSM 1690 / IMET 11303 / Sp7;
RX   PubMed=8026752; DOI=10.1016/0378-1119(94)90221-6;
RA   de Troch P., Keijers V., Vanderleyden J.;
RT   "Sequence analysis of the Azospirillum brasilense exoB gene, encoding UDP-
RT   glucose 4'-epimerase.";
RL   Gene 144:143-144(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z25478; CAA80967.1; -; Genomic_DNA.
DR   PIR; I39490; I39490.
DR   RefSeq; WP_059399823.1; NZ_VITX01000018.1.
DR   AlphaFoldDB; Q59083; -.
DR   SMR; Q59083; -.
DR   GeneID; 56449505; -.
DR   OrthoDB; 1597849at2; -.
DR   UniPathway; UPA00214; -.
DR   UniPathway; UPA00631; -.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR   InterPro; IPR001509; Epimerase_deHydtase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005886; UDP_G4E.
DR   Pfam; PF01370; Epimerase; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01179; galE; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Exopolysaccharide synthesis; Galactose metabolism;
KW   Isomerase; NAD; Plasmid.
FT   CHAIN           1..348
FT                   /note="UDP-glucose 4-epimerase"
FT                   /id="PRO_0000183226"
FT   ACT_SITE        149
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         125
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   348 AA;  36701 MW;  9517B6B2A180FBA0 CRC64;
     MTDQTAASPR VLVTGGAGYI GSHVLHALTD AGIPAVTIDD LSAGRREAIP AAVPLVEGDI
     GSAELLDRVM RDHRVDAVMH FAGSIVVPES VVKPLDYYRN NTANSLTLLG ACLRAGIDKV
     VFSSTAAVYG APESVPIRED APTVPINPYG ASKLMTEQML RDAGAAHGLR SVILRYFNVA
     GADPAGRTGQ ATPVATHLIK VACQALLGRR PPLAIFGTDY DTPDGTCIRD YIHVSDLADA
     HVLALLHLRR GGGSLLMNCG YGRGASVREV VRTLEEVSGE QVPATFADRR PGDPPQLVAG
     ADRIREQLGW VPKHDRLDGI VRSALSWERS LEQSVGQAGA PGGSASRS