ID   EXOB_RHIME              Reviewed;         328 AA.
AC   P26503; O54058; P72293;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=UDP-glucose 4-epimerase;
DE            EC=5.1.3.2;
DE   AltName: Full=Galactowaldenase;
GN   Name=exoB; OrderedLocusNames=RB1065; ORFNames=SMb20942;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RCR2011 / SU47;
RX   PubMed=1787800; DOI=10.1111/j.1365-2958.1991.tb00799.x;
RA   Buendia A.M., Enenkel B., Koeplin R., Niehaus K., Arnold W., Puehler A.;
RT   "The Rhizobium meliloti exoZl exoB fragment of megaplasmid 2: ExoB
RT   functions as a UDP-glucose 4-epimerase and ExoZ shows homology to NodX of
RT   Rhizobium leguminosarum biovar viciae strain TOM.";
RL   Mol. Microbiol. 5:1519-1530(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43.
RC   STRAIN=RCR2011 / SU47;
RX   PubMed=8544814; DOI=10.1007/bf00290574;
RA   Becker A., Kuester H., Niehaus K., Puehler A.;
RT   "Extension of the Rhizobium meliloti succinoglycan biosynthesis gene
RT   cluster: identification of the exsA gene encoding an ABC transporter
RT   protein, and the exsB gene which probably codes for a regulator of
RT   succinoglycan biosynthesis.";
RL   Mol. Gen. Genet. 249:487-497(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC   -!- PATHWAY: Carbohydrate metabolism; galactose metabolism.
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; X58126; CAA41127.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49465.1; -; Genomic_DNA.
DR   EMBL; Z50189; CAA90567.1; -; Genomic_DNA.
DR   EMBL; AJ225561; CAA12528.1; -; Genomic_DNA.
DR   PIR; A95975; A95975.
DR   PIR; S16300; S16300.
DR   RefSeq; NP_437605.1; NC_003078.1.
DR   RefSeq; WP_010975903.1; NC_003078.1.
DR   AlphaFoldDB; P26503; -.
DR   SMR; P26503; -.
DR   STRING; 266834.SM_b20942; -.
DR   EnsemblBacteria; CAC49465; CAC49465; SM_b20942.
DR   GeneID; 61601022; -.
DR   KEGG; sme:SM_b20942; -.
DR   PATRIC; fig|266834.11.peg.5994; -.
DR   eggNOG; COG1087; Bacteria.
DR   HOGENOM; CLU_007383_1_10_5; -.
DR   OMA; KVMTGQY; -.
DR   UniPathway; UPA00214; -.
DR   UniPathway; UPA00631; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd05247; UDP_G4E_1_SDR_e; 1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005886; UDP_G4E.
DR   Pfam; PF16363; GDP_Man_Dehyd; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01179; galE; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Exopolysaccharide synthesis; Galactose metabolism;
KW   Isomerase; NAD; Plasmid; Reference proteome.
FT   CHAIN           1..328
FT                   /note="UDP-glucose 4-epimerase"
FT                   /id="PRO_0000183228"
FT   ACT_SITE        143
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         119
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   328 AA;  36113 MW;  A798EA8017C7265E CRC64;
     MQNNNILVVG GAGYIGSHTC LQLAAKGYQP VVYDNLSNGH EEFVKWGVLE KGDIRDRQRL
     DEVLARHKPR AILHFAAMIE VGESVKDPAA FYDNNVIGTL TLLSAALAAG IDAFVFSSTC
     ATYGLPDSVP MDESHKQAPI NPYGRTKWIC EQALKDYGLY KGLRSVILRY FNAAGADFEG
     RIGEWHEPET HAIPLAIDAA LGRREGFKVF GTDYDTRDGT CVRDYIHVLD LADAHVRAVD
     YLLEGGESVA LNLGTGTGTT VKELLDAIEK VAKRPFNIGY AERREGDSTT LVANNDKARQ
     VLGWEPQYDL AAITESAWNW HSRRNQGG