EXOC1_DROME
ID EXOC1_DROME Reviewed; 889 AA.
AC Q9VVG4; Q95R79;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Exocyst complex component 1;
DE AltName: Full=Exocyst complex component Sec3;
GN Name=Sec3 {ECO:0000312|FlyBase:FBgn0266669};
GN ORFNames=CG3885 {ECO:0000312|FlyBase:FBgn0266669};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-145; SER-148 AND SER-456, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane.
CC {ECO:0000250}.
CC -!- SUBUNIT: The exocyst complex is composed of Sec3/Exoc1, Sec5/Exoc2,
CC Sec6/Exoc3, Sec8/Exoc4, Sec10/Exoc5, Sec15/Exoc6, Exo70/Exoc7 and
CC Exo84/Exoc8. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SEC3 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF49347.2; -; Genomic_DNA.
DR EMBL; AY061578; AAL29126.1; -; mRNA.
DR RefSeq; NP_648976.2; NM_140719.4.
DR AlphaFoldDB; Q9VVG4; -.
DR SMR; Q9VVG4; -.
DR BioGRID; 65230; 9.
DR DIP; DIP-20430N; -.
DR IntAct; Q9VVG4; 8.
DR STRING; 7227.FBpp0074993; -.
DR iPTMnet; Q9VVG4; -.
DR PaxDb; Q9VVG4; -.
DR PRIDE; Q9VVG4; -.
DR EnsemblMetazoa; FBtr0075231; FBpp0074993; FBgn0266669.
DR GeneID; 39940; -.
DR KEGG; dme:Dmel_CG3885; -.
DR UCSC; CG3885-RA; d. melanogaster.
DR CTD; 39940; -.
DR FlyBase; FBgn0266669; Sec3.
DR VEuPathDB; VectorBase:FBgn0266669; -.
DR eggNOG; KOG2148; Eukaryota.
DR HOGENOM; CLU_015381_1_0_1; -.
DR InParanoid; Q9VVG4; -.
DR OMA; KEAKKCY; -.
DR OrthoDB; 201698at2759; -.
DR PhylomeDB; Q9VVG4; -.
DR Reactome; R-DME-264876; Insulin processing.
DR Reactome; R-DME-5620916; VxPx cargo-targeting to cilium.
DR SignaLink; Q9VVG4; -.
DR BioGRID-ORCS; 39940; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39940; -.
DR PRO; PR:Q9VVG4; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0266669; Expressed in embryonic/larval hemocyte (Drosophila) and 24 other tissues.
DR ExpressionAtlas; Q9VVG4; baseline and differential.
DR Genevisible; Q9VVG4; DM.
DR GO; GO:0000145; C:exocyst; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; ISS:FlyBase.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR028258; Sec3-PIP2_bind.
DR InterPro; IPR019160; Sec3_C.
DR Pfam; PF15277; Sec3-PIP2_bind; 1.
DR Pfam; PF09763; Sec3_C; 1.
DR SMART; SM01313; Sec3-PIP2_bind; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Exocytosis; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..889
FT /note="Exocyst complex component 1"
FT /id="PRO_0000118916"
FT COILED 156..269
FT /evidence="ECO:0000255"
FT MOD_RES 145
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 148
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 456
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 92
FT /note="E -> Q (in Ref. 3; AAL29126)"
FT /evidence="ECO:0000305"
FT CONFLICT 720
FT /note="V -> I (in Ref. 3; AAL29126)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 889 AA; 102695 MW; 4C9D5E5298D65B78 CRC64;
MLSIGAMANI KHTLQKELFL ASGERLLSVV TVVKKKDKKP CYLCVVTTAP PVPVVTLCLI
KQSEQREGEY KRKRSWQLDE IKWVDGRNEQ FETHEFDLQL EKLYKWYALN PHERQNFLAV
LNRQIQKSVR GQRAEFRNVP AAWLSEKSPE KVALGRAVQK TQHMDDEEDE EEEAQEFTAL
TDKEANELGK LFSECDFAIK DAEQFIEQLS RELHDLDGAN MQSVLASEQK VLKMMEHIDN
AISEADKFEN RLDSYEDILG HVKETMEKIG GKNAMIEIAN NNNIKLMKEL NKVISQLDLP
HSQQQALDEP DLKTANGRKA AIAAAQCLQQ AMNSDIDPAL LRLEAVQDQR KRFEKWKQKF
SATVSRFMNN LFIHLGNEIG DMQVTSTELT LPNHSNVHRE LTPYTELMHW TKAMDRKTYD
GLMRVYTASL SKIYDRDVRN FFNLAKIQVT EKLRNSREDL DMSTSSRKSA VSTIPYGTLG
INRDQWGPGV ETADRMRFDA LLEKVLAELE PIALQEQLFC INFFQMDVIS PTTKNTQTTL
EMEKAVDMTQ SIISGAVSPS GDGVPQKRID RQINEDVRKL MMGLFGCLEP ELVSFIQSFE
RVDSFYSLYV FVRLTQHVMS AQDTHSFLSM TFASALVQVK RSFDRFMQNQ LLSIREAKLH
KRSKAILPYV ENFENFAQTA EGIFRKSDRR TDMEKWYLQL VNAIFEGIQL HSQEHPKTPV
QVVRMENYHH MYALLAQLKV PGLDALKKEA KKCYNDALKA YVTQYFGRPL EKLNQFFEGV
QLKVAQGVKE TEISYQMAFS KQELRKVIAQ YPAREVKKGL ENLYKKVEKH LSEEENLLQV
VWHAMQEEFI AQYNYLEERI QKCYAGAMIN LEFNIQDILA FFSDIARSH
