EXOC3_HUMAN
ID EXOC3_HUMAN Reviewed; 745 AA.
AC O60645; Q6P2E8; Q8TEN6; Q8WUW0; Q96DI4;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 10-APR-2019, sequence version 3.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Exocyst complex component 3;
DE AltName: Full=Exocyst complex component Sec6;
GN Name=EXOC3; Synonyms=SEC6, SEC6L1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Epididymis;
RX PubMed=20736409; DOI=10.1074/mcp.m110.001719;
RA Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C.,
RA Jin S., Liu J., Zhu P., Liu Y.;
RT "Systematic mapping and functional analysis of a family of human epididymal
RT secretory sperm-located proteins.";
RL Mol. Cell. Proteomics 9:2517-2528(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=12693554; DOI=10.1093/dnares/10.1.49;
RA Jikuya H., Takano J., Kikuno R., Hirosawa M., Nagase T., Nomura N.,
RA Ohara O.;
RT "Characterization of long cDNA clones from human adult spleen. II. The
RT complete sequences of 81 cDNA clones.";
RL DNA Res. 10:49-57(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon, Lung, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 275-745.
RC TISSUE=Brain;
RA Yu W., Gibbs R.A.;
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH BIRC6.
RX PubMed=18329369; DOI=10.1016/j.cell.2008.01.012;
RA Pohl C., Jentsch S.;
RT "Final stages of cytokinesis and midbody ring formation are controlled by
RT BRUCE.";
RL Cell 132:832-845(2008).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=18756269; DOI=10.1038/emboj.2008.166;
RA Cascone I., Selimoglu R., Ozdemir C., Del Nery E., Yeaman C., White M.,
RA Camonis J.;
RT "Distinct roles of RalA and RalB in the progression of cytokinesis are
RT supported by distinct RalGEFs.";
RL EMBO J. 27:2375-2387(2008).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-28, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane.
CC -!- SUBUNIT: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4,
CC EXOC5, EXOC6, EXOC7 and EXOC8 (By similarity). Interacts with EXOC3L1
CC (By similarity). Interacts with BIRC6/bruce. Interacts with MYRIP (By
CC similarity). Interacts with SLC6A9 (By similarity).
CC {ECO:0000250|UniProtKB:O54921, ECO:0000250|UniProtKB:Q62825,
CC ECO:0000250|UniProtKB:Q6KAR6, ECO:0000269|PubMed:18329369}.
CC -!- INTERACTION:
CC O60645; A1L4K1: FSD2; NbExp=3; IntAct=EBI-1052278, EBI-5661036;
CC O60645; Q96IK5: GMCL1; NbExp=3; IntAct=EBI-1052278, EBI-2548508;
CC O60645; Q9BZL4: PPP1R12C; NbExp=3; IntAct=EBI-1052278, EBI-721802;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O54921}.
CC Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O54921}. Cell
CC projection, growth cone {ECO:0000250|UniProtKB:O54921}. Midbody
CC {ECO:0000269|PubMed:18756269}. Golgi apparatus
CC {ECO:0000305|PubMed:18756269}. Cell projection, neuron projection
CC {ECO:0000250|UniProtKB:Q62825}. Note=Perinuclear in undifferentiated
CC cells. Redistributes to growing neurites and growth cones during
CC neuronal differentiation (By similarity). During mitosis, early
CC recruitment to the midbody requires RALA, but not RALB, and EXOC2. In
CC late stages of cytokinesis, localization to the midbody is RALB-
CC dependent (PubMed:18756269). {ECO:0000250|UniProtKB:O54921,
CC ECO:0000269|PubMed:18756269}.
CC -!- TISSUE SPECIFICITY: Expressed in epididymis (at protein level).
CC {ECO:0000269|PubMed:20736409}.
CC -!- SIMILARITY: Belongs to the SEC6 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB84912.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; GU727644; ADU87645.1; -; mRNA.
DR EMBL; AK074086; BAB84912.1; ALT_SEQ; mRNA.
DR EMBL; AC010442; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471235; EAW51000.1; -; Genomic_DNA.
DR EMBL; BC001511; AAH01511.2; -; mRNA.
DR EMBL; BC019304; AAH19304.1; -; mRNA.
DR EMBL; BC064569; AAH64569.1; -; mRNA.
DR EMBL; AF055006; AAC09358.1; -; mRNA.
DR CCDS; CCDS54830.1; -.
DR RefSeq; NP_009208.2; NM_007277.4.
DR RefSeq; XP_016864492.1; XM_017009003.1.
DR RefSeq; XP_016864493.1; XM_017009004.1.
DR AlphaFoldDB; O60645; -.
DR SMR; O60645; -.
DR BioGRID; 116464; 137.
DR ComplexPortal; CPX-4943; Exocyst, EXOC6 variant.
DR ComplexPortal; CPX-4944; Exocyst, EXOC6B variant.
DR CORUM; O60645; -.
DR IntAct; O60645; 50.
DR MINT; O60645; -.
DR STRING; 9606.ENSP00000425587; -.
DR TCDB; 1.F.2.1.2; the octameric exocyst (exocyst) family.
DR iPTMnet; O60645; -.
DR PhosphoSitePlus; O60645; -.
DR BioMuta; EXOC3; -.
DR EPD; O60645; -.
DR jPOST; O60645; -.
DR MassIVE; O60645; -.
DR MaxQB; O60645; -.
DR PaxDb; O60645; -.
DR PeptideAtlas; O60645; -.
DR PRIDE; O60645; -.
DR ProteomicsDB; 66896; -.
DR Antibodypedia; 22230; 145 antibodies from 27 providers.
DR DNASU; 11336; -.
DR Ensembl; ENST00000315013.9; ENSP00000323377.5; ENSG00000180104.16.
DR Ensembl; ENST00000512944.6; ENSP00000425587.1; ENSG00000180104.16.
DR GeneID; 11336; -.
DR KEGG; hsa:11336; -.
DR MANE-Select; ENST00000512944.6; ENSP00000425587.1; NM_007277.5; NP_009208.2.
DR UCSC; uc003jba.4; human.
DR CTD; 11336; -.
DR DisGeNET; 11336; -.
DR GeneCards; EXOC3; -.
DR HGNC; HGNC:30378; EXOC3.
DR HPA; ENSG00000180104; Low tissue specificity.
DR MIM; 608186; gene.
DR neXtProt; NX_O60645; -.
DR OpenTargets; ENSG00000180104; -.
DR PharmGKB; PA134869816; -.
DR VEuPathDB; HostDB:ENSG00000180104; -.
DR eggNOG; KOG2286; Eukaryota.
DR GeneTree; ENSGT01030000234613; -.
DR HOGENOM; CLU_016260_1_0_1; -.
DR InParanoid; O60645; -.
DR OMA; MNIGPKT; -.
DR PhylomeDB; O60645; -.
DR TreeFam; TF314979; -.
DR PathwayCommons; O60645; -.
DR Reactome; R-HSA-1445148; Translocation of SLC2A4 (GLUT4) to the plasma membrane.
DR Reactome; R-HSA-264876; Insulin processing.
DR Reactome; R-HSA-5620916; VxPx cargo-targeting to cilium.
DR SignaLink; O60645; -.
DR BioGRID-ORCS; 11336; 552 hits in 1081 CRISPR screens.
DR ChiTaRS; EXOC3; human.
DR GeneWiki; EXOC3; -.
DR GenomeRNAi; 11336; -.
DR Pharos; O60645; Tbio.
DR PRO; PR:O60645; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; O60645; protein.
DR Bgee; ENSG00000180104; Expressed in mucosa of transverse colon and 200 other tissues.
DR ExpressionAtlas; O60645; baseline and differential.
DR Genevisible; O60645; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0000145; C:exocyst; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0030426; C:growth cone; IEA:UniProtKB-SubCell.
DR GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042734; C:presynaptic membrane; IEA:Ensembl.
DR GO; GO:0030667; C:secretory granule membrane; TAS:Reactome.
DR GO; GO:0045296; F:cadherin binding; HDA:BHF-UCL.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0051601; P:exocyst localization; IBA:GO_Central.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0090148; P:membrane fission; IC:ComplexPortal.
DR GO; GO:0000281; P:mitotic cytokinesis; IC:ComplexPortal.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; IC:ComplexPortal.
DR GO; GO:0090522; P:vesicle tethering involved in exocytosis; IC:ComplexPortal.
DR Gene3D; 1.10.357.70; -; 1.
DR InterPro; IPR010326; EXOC3/Sec6.
DR InterPro; IPR042532; EXOC3/Sec6_C.
DR PANTHER; PTHR21292; PTHR21292; 1.
DR Pfam; PF06046; Sec6; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell projection; Cytoplasm; Exocytosis; Golgi apparatus;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..745
FT /note="Exocyst complex component 3"
FT /id="PRO_0000118925"
FT MOD_RES 28
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:19608861"
SQ SEQUENCE 745 AA; 85567 MW; 81D03FB3351A995D CRC64;
MKETDREAVA TAVQRVAGML QRPDQLDKVE QYRRREARKK ASVEARLKAA IQSQLDGVRT
GLSQLHNALN DVKDIQQSLA DVSKDWRQSI NTIESLKDVK DAVVQHSQLA AAVENLKNIF
SVPEIVRETQ DLIEQGALLQ AHRKLMDLEC SRDGLMYEQY RMDSGNTRDM TLIHGYFGST
QGLSDELAKQ LWMVLQRSLV TVRRDPTLLV SVVRIIEREE KIDRRILDRK KQTGFVPPGR
PKNWKEKMFT ILERTVTTRI EGTQADTRES DKMWLVRHLE IIRKYVLDDL IVAKNLMVQC
FPPHYEIFKN LLNMYHQALS TRMQDLASED LEANEIVSLL TWVLNTYTST EMMRNVELAP
EVDVGTLEPL LSPHVVSELL DTYMSTLTSN IIAWLRKALE TDKKDWVKET EPEADQDGYY
QTTLPAIVFQ MFEQNLQVAA QISEDLKTKV LVLCLQQMNS FLSRYKDEAQ LYKEEHLRNR
QHPHCYVQYM IAIINNCQTF KESIVSLKRK YLKNEVEEGV SPSQPSMDGI LDAIAKEGCS
GLLEEVFLDL EQHLNELMTK KWLLGSNAVD IICVTVEDYF NDFAKIKKPY KKRMTAEAHR
RVVVEYLRAV MQKRISFRSP EERKEGAEKM VREAEQLRFL FRKLASGFGE DVDGYCDTIV
AVAEVIKLTD PSLLYLEVST LVSKYPDIRD DHIGALLAVR GDASRDMKQT IMETLEQGPA
QASPSYVPLF KDIVVPSLNV AKLLK
