EXOC3_MOUSE
ID EXOC3_MOUSE Reviewed; 755 AA.
AC Q6KAR6;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Exocyst complex component 3;
DE AltName: Full=Exocyst complex component Sec6;
GN Name=Exoc3; Synonyms=Sec6l1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [2]
RP INTERACTION WITH MYRIP.
RX PubMed=17827149; DOI=10.1074/jbc.m705167200;
RA Goehring A.S., Pedroja B.S., Hinke S.A., Langeberg L.K., Scott J.D.;
RT "MyRIP anchors protein kinase A to the exocyst complex.";
RL J. Biol. Chem. 282:33155-33167(2007).
RN [3]
RP INTERACTION WITH EXOC3L1.
RX PubMed=18480549; DOI=10.2220/biomedres.29.85;
RA Saito T., Shibasaki T., Seino S.;
RT "Involvement of Exoc3l, a protein structurally related to the exocyst
RT subunit Sec6, in insulin secretion.";
RL Biomed. Res. 29:85-91(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane.
CC {ECO:0000250}.
CC -!- SUBUNIT: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4,
CC EXOC5, EXOC6, EXOC7 and EXOC8 (By similarity). Interacts with EXOC3L1
CC (PubMed:18480549). Interacts with BIRC6/bruce (By similarity).
CC Interacts with MYRIP (PubMed:17827149). Interacts with SLC6A9 (By
CC similarity). {ECO:0000250|UniProtKB:O54921,
CC ECO:0000250|UniProtKB:Q62825, ECO:0000269|PubMed:17827149,
CC ECO:0000269|PubMed:18480549}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O54921}.
CC Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O54921}. Cell
CC projection, growth cone {ECO:0000250|UniProtKB:O54921}. Midbody
CC {ECO:0000250|UniProtKB:O60645}. Golgi apparatus
CC {ECO:0000250|UniProtKB:O60645}. Cell projection, neuron projection
CC {ECO:0000250|UniProtKB:Q62825}. Note=Perinuclear in undifferentiated
CC cells. Redistributes to growing neurites and growth cones during
CC neuronal differentiation (By similarity). During mitosis, early
CC recruitment to the midbody requires RALA, but not RALB, and EXOC2. In
CC late stages of cytokinesis, localization to the midbody is RALB-
CC dependent (By similarity). {ECO:0000250|UniProtKB:O54921,
CC ECO:0000250|UniProtKB:O60645}.
CC -!- SIMILARITY: Belongs to the SEC6 family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-11 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD21391.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK131141; BAD21391.1; ALT_INIT; mRNA.
DR CCDS; CCDS26640.1; -.
DR RefSeq; NP_796307.2; NM_177333.3.
DR RefSeq; XP_006517254.1; XM_006517191.2.
DR AlphaFoldDB; Q6KAR6; -.
DR SMR; Q6KAR6; -.
DR BioGRID; 229233; 7.
DR ComplexPortal; CPX-4982; Exocyst, Exoc6 variant.
DR ComplexPortal; CPX-4983; Exocyst, Exoc6b variant.
DR IntAct; Q6KAR6; 5.
DR MINT; Q6KAR6; -.
DR STRING; 10090.ENSMUSP00000039416; -.
DR iPTMnet; Q6KAR6; -.
DR PhosphoSitePlus; Q6KAR6; -.
DR EPD; Q6KAR6; -.
DR PaxDb; Q6KAR6; -.
DR PeptideAtlas; Q6KAR6; -.
DR PRIDE; Q6KAR6; -.
DR ProteomicsDB; 275487; -.
DR Antibodypedia; 22230; 145 antibodies from 27 providers.
DR DNASU; 211446; -.
DR Ensembl; ENSMUST00000035934; ENSMUSP00000039416; ENSMUSG00000034152.
DR GeneID; 211446; -.
DR KEGG; mmu:211446; -.
DR UCSC; uc007reu.1; mouse.
DR CTD; 11336; -.
DR MGI; MGI:2443972; Exoc3.
DR VEuPathDB; HostDB:ENSMUSG00000034152; -.
DR eggNOG; KOG2286; Eukaryota.
DR GeneTree; ENSGT01030000234613; -.
DR HOGENOM; CLU_016260_1_0_1; -.
DR InParanoid; Q6KAR6; -.
DR OMA; MNIGPKT; -.
DR OrthoDB; 391172at2759; -.
DR PhylomeDB; Q6KAR6; -.
DR TreeFam; TF314979; -.
DR Reactome; R-MMU-264876; Insulin processing.
DR Reactome; R-MMU-5620916; VxPx cargo-targeting to cilium.
DR BioGRID-ORCS; 211446; 22 hits in 59 CRISPR screens.
DR ChiTaRS; Exoc3; mouse.
DR PRO; PR:Q6KAR6; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q6KAR6; protein.
DR Bgee; ENSMUSG00000034152; Expressed in manus and 222 other tissues.
DR ExpressionAtlas; Q6KAR6; baseline and differential.
DR Genevisible; Q6KAR6; MM.
DR GO; GO:0000145; C:exocyst; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0030426; C:growth cone; IEA:UniProtKB-SubCell.
DR GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042734; C:presynaptic membrane; ISO:MGI.
DR GO; GO:0030667; C:secretory granule membrane; TAS:Reactome.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0051601; P:exocyst localization; IBA:GO_Central.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0090148; P:membrane fission; IC:ComplexPortal.
DR GO; GO:0000281; P:mitotic cytokinesis; IC:ComplexPortal.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; IC:ComplexPortal.
DR GO; GO:0090522; P:vesicle tethering involved in exocytosis; IC:ComplexPortal.
DR Gene3D; 1.10.357.70; -; 1.
DR InterPro; IPR010326; EXOC3/Sec6.
DR InterPro; IPR042532; EXOC3/Sec6_C.
DR PANTHER; PTHR21292; PTHR21292; 1.
DR Pfam; PF06046; Sec6; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell projection; Cytoplasm; Exocytosis; Golgi apparatus;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..755
FT /note="Exocyst complex component 3"
FT /id="PRO_0000118926"
FT MOD_RES 38
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:O60645"
SQ SEQUENCE 755 AA; 86455 MW; 47B4D8AFAAC7CBCB CRC64;
MCKDSACFLT MKETDLEAVA TAVQRVAGML QRPDQLDKVE QYRRREARKK ASVEARLKAA
IQSQLDGVRT GLSQLHNALN DVKDIQQSLA DVSKDWRQSI NTIESLKDVK DAVVQHSQLA
AAVENLKNIF SVPEIVRETQ DLIEQGALLQ AHRKLMDLEC SRDGLMCEQY RMDSGNKRDM
TLIHGYFGST QGLSDELAKQ LWMVLQRSLV TVRRDPTLLV SVVRIIEREE KIDRRILDRK
KQTGFVPPGR PKNWKEKMFA ILDRTVTTRI EGTQADTRES DKMWLVRHLE IIRKYVLDDL
IVAKNLMVQC FPPHYEIFKN LLSMYHQALS TRMQDLASED LEANEIVSLL TWVLNTYTSA
EMMGNVELAP EVDVSALEPL LSPNIVSELL DTYMSTLTSN IIAWLRKALE TDKKDWSKET
EPEADQDGYY QTTLPAIVFQ MFEQNLQVAA QISEDLKTKV LVLCLQQMNS FLSRYKDEAQ
LYKEEHLRNR QHPHCYVQYM IAIINNCQTF KESIISLKRK YLKTEAEEGL CLSQPSMDGI
LDAIAKEGCS SLLEEVFLDL EQHLNELMTK KWLLGSNAVD IICVTVEDYF NDFAKIKKPY
KKRMTAEAHR RVVVEYLRAV MQKRISFRSA EERKEGAEKM VREAEQLRFL FRKLASGFGE
DADGHCDTIV AVAEVIKLTD PSLLYLEVST LVSKYPDIRD DHIGALLALR GDASRDMKQT
IMETLEQGPM QASPNYVPIF KEIVVPSLNV AKLLK
