EXOC5_HUMAN
ID EXOC5_HUMAN Reviewed; 708 AA.
AC O00471; B2R6C5; Q8IW24;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Exocyst complex component 5;
DE AltName: Full=Exocyst complex component Sec10;
DE Short=hSec10;
GN Name=EXOC5; Synonyms=SEC10, SEC10L1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=9119050; DOI=10.1016/s0014-5793(97)00109-9;
RA Guo W., Roth D., Gatti E., De Camilli P., Novick P.;
RT "Identification and characterization of homologues of the exocyst component
RT Sec10p.";
RL FEBS Lett. 404:135-139(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=18756269; DOI=10.1038/emboj.2008.166;
RA Cascone I., Selimoglu R., Ozdemir C., Del Nery E., Yeaman C., White M.,
RA Camonis J.;
RT "Distinct roles of RalA and RalB in the progression of cytokinesis are
RT supported by distinct RalGEFs.";
RL EMBO J. 27:2375-2387(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane.
CC -!- SUBUNIT: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4,
CC EXOC5, EXOC6, EXOC7 and EXOC8 (By similarity). Interacts with EXOC3L1
CC (By similarity). {ECO:0000250|UniProtKB:P97878,
CC ECO:0000250|UniProtKB:Q3TPX4}.
CC -!- INTERACTION:
CC O00471; A0AVN2: BARD1; NbExp=3; IntAct=EBI-949824, EBI-9977322;
CC O00471; Q99728: BARD1; NbExp=3; IntAct=EBI-949824, EBI-473181;
CC O00471; P23560-2: BDNF; NbExp=3; IntAct=EBI-949824, EBI-12275524;
CC O00471; Q5PSV4: BRMS1L; NbExp=3; IntAct=EBI-949824, EBI-5666615;
CC O00471; Q8TAB5: C1orf216; NbExp=3; IntAct=EBI-949824, EBI-747505;
CC O00471; Q68D86: CCDC102B; NbExp=3; IntAct=EBI-949824, EBI-10171570;
CC O00471; P02511: CRYAB; NbExp=3; IntAct=EBI-949824, EBI-739060;
CC O00471; P53674: CRYBB1; NbExp=3; IntAct=EBI-949824, EBI-7519424;
CC O00471; Q05D60: DEUP1; NbExp=11; IntAct=EBI-949824, EBI-748597;
CC O00471; Q3B7T1-5: EDRF1; NbExp=3; IntAct=EBI-949824, EBI-10240074;
CC O00471; Q6FHV6: ENO2; NbExp=3; IntAct=EBI-949824, EBI-10249724;
CC O00471; Q8TAG9: EXOC6; NbExp=6; IntAct=EBI-949824, EBI-1223394;
CC O00471; Q9Y2D4: EXOC6B; NbExp=6; IntAct=EBI-949824, EBI-2690026;
CC O00471; P02671-2: FGA; NbExp=3; IntAct=EBI-949824, EBI-9640259;
CC O00471; Q96NE9: FRMD6; NbExp=3; IntAct=EBI-949824, EBI-741729;
CC O00471; P04406: GAPDH; NbExp=3; IntAct=EBI-949824, EBI-354056;
CC O00471; Q8N4P3: HDDC3; NbExp=3; IntAct=EBI-949824, EBI-750003;
CC O00471; A0A0S2Z4Q4: HGS; NbExp=3; IntAct=EBI-949824, EBI-16429135;
CC O00471; O14964: HGS; NbExp=3; IntAct=EBI-949824, EBI-740220;
CC O00471; P42858: HTT; NbExp=3; IntAct=EBI-949824, EBI-466029;
CC O00471; Q9NWB7: IFT57; NbExp=3; IntAct=EBI-949824, EBI-725672;
CC O00471; Q9BVG8-5: KIFC3; NbExp=3; IntAct=EBI-949824, EBI-14069005;
CC O00471; Q9BQD3: KXD1; NbExp=3; IntAct=EBI-949824, EBI-739657;
CC O00471; P43357: MAGEA3; NbExp=3; IntAct=EBI-949824, EBI-5651459;
CC O00471; P43360: MAGEA6; NbExp=6; IntAct=EBI-949824, EBI-1045155;
CC O00471; P00973: OAS1; NbExp=3; IntAct=EBI-949824, EBI-3932815;
CC O00471; P00973-2: OAS1; NbExp=6; IntAct=EBI-949824, EBI-12081862;
CC O00471; D3DTS7: PMP22; NbExp=3; IntAct=EBI-949824, EBI-25882629;
CC O00471; A0A0S2Z528: PSTPIP1; NbExp=3; IntAct=EBI-949824, EBI-16430249;
CC O00471; Q13882: PTK6; NbExp=6; IntAct=EBI-949824, EBI-1383632;
CC O00471; Q9ULZ3: PYCARD; NbExp=5; IntAct=EBI-949824, EBI-751215;
CC O00471; P61106: RAB14; NbExp=6; IntAct=EBI-949824, EBI-1056404;
CC O00471; P20338: RAB4A; NbExp=6; IntAct=EBI-949824, EBI-722284;
CC O00471; P61018: RAB4B; NbExp=6; IntAct=EBI-949824, EBI-10218066;
CC O00471; P37840: SNCA; NbExp=3; IntAct=EBI-949824, EBI-985879;
CC O00471; Q96E40: SPACA9; NbExp=3; IntAct=EBI-949824, EBI-722584;
CC O00471; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-949824, EBI-5235340;
CC O00471; Q9UNE7: STUB1; NbExp=3; IntAct=EBI-949824, EBI-357085;
CC O00471; Q13148: TARDBP; NbExp=6; IntAct=EBI-949824, EBI-372899;
CC O00471; Q96FV9: THOC1; NbExp=3; IntAct=EBI-949824, EBI-1765605;
CC O00471; O14787: TNPO2; NbExp=4; IntAct=EBI-949824, EBI-431907;
CC O00471; Q86WV8: TSC1; NbExp=3; IntAct=EBI-949824, EBI-12806590;
CC O00471; Q6PF05-3: TTC23L; NbExp=3; IntAct=EBI-949824, EBI-10182647;
CC O00471; Q8N3L3: TXLNB; NbExp=4; IntAct=EBI-949824, EBI-6116822;
CC O00471; P83876: TXNL4A; NbExp=3; IntAct=EBI-949824, EBI-746539;
CC O00471; O75604: USP2; NbExp=3; IntAct=EBI-949824, EBI-743272;
CC O00471; O76024: WFS1; NbExp=3; IntAct=EBI-949824, EBI-720609;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9119050}. Midbody
CC {ECO:0000269|PubMed:18756269}. Note=Localization at the midbody
CC requires the presence of RALA, EXOC2 and EXOC3.
CC {ECO:0000269|PubMed:18756269}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:9119050}.
CC -!- SIMILARITY: Belongs to the SEC10 family. {ECO:0000305}.
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DR EMBL; U85946; AAB53388.1; -; mRNA.
DR EMBL; AK312523; BAG35422.1; -; mRNA.
DR EMBL; CH471061; EAW80699.1; -; Genomic_DNA.
DR EMBL; BC041126; AAH41126.1; -; mRNA.
DR CCDS; CCDS45111.1; -.
DR RefSeq; NP_006535.1; NM_006544.3.
DR AlphaFoldDB; O00471; -.
DR SMR; O00471; -.
DR BioGRID; 115884; 120.
DR ComplexPortal; CPX-4943; Exocyst, EXOC6 variant.
DR ComplexPortal; CPX-4944; Exocyst, EXOC6B variant.
DR CORUM; O00471; -.
DR IntAct; O00471; 87.
DR MINT; O00471; -.
DR STRING; 9606.ENSP00000484855; -.
DR TCDB; 1.F.2.1.2; the octameric exocyst (exocyst) family.
DR iPTMnet; O00471; -.
DR PhosphoSitePlus; O00471; -.
DR BioMuta; EXOC5; -.
DR EPD; O00471; -.
DR jPOST; O00471; -.
DR MassIVE; O00471; -.
DR MaxQB; O00471; -.
DR PaxDb; O00471; -.
DR PeptideAtlas; O00471; -.
DR PRIDE; O00471; -.
DR ProteomicsDB; 47919; -.
DR Antibodypedia; 24095; 127 antibodies from 26 providers.
DR DNASU; 10640; -.
DR Ensembl; ENST00000621441.5; ENSP00000484855.1; ENSG00000070367.16.
DR GeneID; 10640; -.
DR KEGG; hsa:10640; -.
DR MANE-Select; ENST00000621441.5; ENSP00000484855.1; NM_006544.4; NP_006535.1.
DR UCSC; uc001xct.4; human.
DR CTD; 10640; -.
DR DisGeNET; 10640; -.
DR GeneCards; EXOC5; -.
DR HGNC; HGNC:10696; EXOC5.
DR HPA; ENSG00000070367; Low tissue specificity.
DR MIM; 604469; gene.
DR neXtProt; NX_O00471; -.
DR OpenTargets; ENSG00000070367; -.
DR PharmGKB; PA35619; -.
DR VEuPathDB; HostDB:ENSG00000070367; -.
DR eggNOG; KOG3745; Eukaryota.
DR GeneTree; ENSGT00390000012837; -.
DR HOGENOM; CLU_020771_1_0_1; -.
DR InParanoid; O00471; -.
DR OMA; PLCKHHY; -.
DR OrthoDB; 225235at2759; -.
DR PhylomeDB; O00471; -.
DR TreeFam; TF314966; -.
DR PathwayCommons; O00471; -.
DR Reactome; R-HSA-1445148; Translocation of SLC2A4 (GLUT4) to the plasma membrane.
DR Reactome; R-HSA-264876; Insulin processing.
DR Reactome; R-HSA-5620916; VxPx cargo-targeting to cilium.
DR SignaLink; O00471; -.
DR BioGRID-ORCS; 10640; 344 hits in 1074 CRISPR screens.
DR ChiTaRS; EXOC5; human.
DR GeneWiki; EXOC5; -.
DR GenomeRNAi; 10640; -.
DR Pharos; O00471; Tbio.
DR PRO; PR:O00471; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; O00471; protein.
DR Bgee; ENSG00000070367; Expressed in cortical plate and 193 other tissues.
DR ExpressionAtlas; O00471; baseline and differential.
DR Genevisible; O00471; HS.
DR GO; GO:0005737; C:cytoplasm; TAS:ProtInc.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0000145; C:exocyst; IBA:GO_Central.
DR GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR GO; GO:0047485; F:protein N-terminus binding; IEA:Ensembl.
DR GO; GO:0031267; F:small GTPase binding; IPI:UniProtKB.
DR GO; GO:1904019; P:epithelial cell apoptotic process; IEA:Ensembl.
DR GO; GO:0001736; P:establishment of planar polarity; IEA:Ensembl.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; IBA:GO_Central.
DR GO; GO:0090148; P:membrane fission; IC:ComplexPortal.
DR GO; GO:0000281; P:mitotic cytokinesis; IC:ComplexPortal.
DR GO; GO:1905515; P:non-motile cilium assembly; IEA:Ensembl.
DR GO; GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:ProtInc.
DR GO; GO:0072659; P:protein localization to plasma membrane; IEA:Ensembl.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; IC:ComplexPortal.
DR GO; GO:0090522; P:vesicle tethering involved in exocytosis; IC:ComplexPortal.
DR InterPro; IPR033960; EXOC5/Sec10.
DR InterPro; IPR009976; Sec10-like.
DR PANTHER; PTHR12100; PTHR12100; 1.
DR PANTHER; PTHR12100:SF4; PTHR12100:SF4; 1.
DR Pfam; PF07393; Sec10; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Cytoplasm; Exocytosis; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..708
FT /note="Exocyst complex component 5"
FT /id="PRO_0000118943"
FT COILED 40..101
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 122
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18691976,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 395
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P97878"
FT MOD_RES 405
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P97878"
FT MOD_RES 412
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P97878"
FT VARIANT 10
FT /note="E -> D (in dbSNP:rs35132458)"
FT /id="VAR_048957"
FT CONFLICT 545
FT /note="G -> R (in Ref. 4; AAH41126)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 708 AA; 81853 MW; CB03AC6C64DAB50C CRC64;
MATTAELFEE PFVADEYIER LVWRTPGGGS RGGPEAFDPK RLLEEFVNHI QELQIMDERI
QRKVEKLEQQ CQKEAKEFAK KVQELQKSNQ VAFQHFQELD EHISYVATKV CHLGDQLEGV
NTPRQRAVEA QKLMKYFNEF LDGELKSDVF TNSEKIKEAA DIIQKLHLIA QELPFDRFSE
VKSKIASKYH DLECQLIQEF TSAQRRGEIS RMREVAAVLL HFKGYSHCVD VYIKQCQEGA
YLRNDIFEDA GILCQRVNKQ VGDIFSNPET VLAKLIQNVF EIKLQSFVKE QLEECRKSDA
EQYLKNLYDL YTRTTNLSSK LMEFNLGTDK QTFLSKLIKS IFISYLENYI EVETGYLKSR
SAMILQRYYD SKNHQKRSIG TGGIQDLKER IRQRTNLPLG PSIDTHGETF LSQEVVVNLL
QETKQAFERC HRLSDPSDLP RNAFRIFTIL VEFLCIEHID YALETGLAGI PSSDSRNANL
YFLDVVQQAN TIFHLFDKQF NDHLMPLISS SPKLSECLQK KKEIIEQMEM KLDTGIDRTL
NCMIGQMKHI LAAEQKKTDF KPEDENNVLI QYTNACVKVC AYVRKQVEKI KNSMDGKNVD
TVLMELGVRF HRLIYEHLQQ YSYSCMGGML AICDVAEYRK CAKDFKIPMV LHLFDTLHAL
CNLLVVAPDN LKQVCSGEQL ANLDKNILHS FVQLRADYRS ARLARHFS
