EXOC6_HUMAN
ID EXOC6_HUMAN Reviewed; 804 AA.
AC Q8TAG9; E9PHI3; Q5VXH8; Q9NZ24;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 3.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Exocyst complex component 6;
DE AltName: Full=Exocyst complex component Sec15A;
DE AltName: Full=SEC15-like protein 1;
GN Name=EXOC6; Synonyms=SEC15A, SEC15L, SEC15L1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ILE-396.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-153 (ISOFORM 1).
RA Li C., Rodriguez M., Banerjee D.;
RT "Subunit structure of the human exocyst complex.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH CNTRL, AND SUBCELLULAR LOCATION.
RX PubMed=16213214; DOI=10.1016/j.cell.2005.07.027;
RA Gromley A., Yeaman C., Rosa J., Redick S., Chen C.-T., Mirabelle S.,
RA Guha M., Sillibourne J., Doxsey S.J.;
RT "Centriolin anchoring of exocyst and SNARE complexes at the midbody is
RT required for secretory-vesicle-mediated abscission.";
RL Cell 123:75-87(2005).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane. Together
CC with RAB11A, RAB3IP, RAB8A, PARD3, PRKCI, ANXA2, CDC42 and DNMBP
CC promotes transcytosis of PODXL to the apical membrane initiation sites
CC (AMIS), apical surface formation and lumenogenesis (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4,
CC EXOC5, EXOC6, EXOC7 and EXOC8 (By similarity). Interacts with CNTRL.
CC Interacts with RAB11A in a GTP-dependent manner (By similarity).
CC {ECO:0000250, ECO:0000250|UniProtKB:O54923}.
CC -!- INTERACTION:
CC Q8TAG9; O00471: EXOC5; NbExp=6; IntAct=EBI-1223394, EBI-949824;
CC Q8TAG9; Q53GS7: GLE1; NbExp=3; IntAct=EBI-1223394, EBI-1955541;
CC Q8TAG9; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-1223394, EBI-5235340;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O54923}.
CC Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:O54923}. Cell
CC projection, growth cone {ECO:0000250|UniProtKB:O54923}. Midbody,
CC Midbody ring {ECO:0000269|PubMed:16213214}. Note=Perinuclear in
CC undifferentiated cells. Redistributes to growing neurites and growth
CC cones during neuronal differentiation. Colocalizes with
CC CNTRL/centriolin at the midbody ring (PubMed:16213214).
CC {ECO:0000250|UniProtKB:O54923, ECO:0000269|PubMed:16213214}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8TAG9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TAG9-2; Sequence=VSP_047160;
CC -!- SIMILARITY: Belongs to the SEC15 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF37262.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL157705; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL358613; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL392103; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL590080; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471066; EAW50083.1; -; Genomic_DNA.
DR EMBL; BC028395; AAH28395.2; -; mRNA.
DR EMBL; AF220217; AAF37262.1; ALT_INIT; mRNA.
DR CCDS; CCDS31247.1; -. [Q8TAG9-2]
DR CCDS; CCDS7424.2; -. [Q8TAG9-1]
DR RefSeq; NP_001013870.1; NM_001013848.3. [Q8TAG9-2]
DR RefSeq; NP_001306123.1; NM_001319194.1.
DR RefSeq; NP_001306124.1; NM_001319195.1.
DR RefSeq; NP_001306129.1; NM_001319200.1.
DR RefSeq; NP_061926.3; NM_019053.5. [Q8TAG9-1]
DR RefSeq; XP_016871834.1; XM_017016345.1. [Q8TAG9-2]
DR AlphaFoldDB; Q8TAG9; -.
DR SMR; Q8TAG9; -.
DR BioGRID; 120023; 75.
DR ComplexPortal; CPX-4943; Exocyst, EXOC6 variant.
DR CORUM; Q8TAG9; -.
DR IntAct; Q8TAG9; 41.
DR MINT; Q8TAG9; -.
DR STRING; 9606.ENSP00000260762; -.
DR TCDB; 1.F.2.1.2; the octameric exocyst (exocyst) family.
DR iPTMnet; Q8TAG9; -.
DR PhosphoSitePlus; Q8TAG9; -.
DR BioMuta; EXOC6; -.
DR DMDM; 212287926; -.
DR EPD; Q8TAG9; -.
DR jPOST; Q8TAG9; -.
DR MassIVE; Q8TAG9; -.
DR MaxQB; Q8TAG9; -.
DR PaxDb; Q8TAG9; -.
DR PeptideAtlas; Q8TAG9; -.
DR PRIDE; Q8TAG9; -.
DR ProteomicsDB; 20542; -.
DR ProteomicsDB; 73883; -. [Q8TAG9-1]
DR Antibodypedia; 45698; 77 antibodies from 22 providers.
DR DNASU; 54536; -.
DR Ensembl; ENST00000260762.10; ENSP00000260762.6; ENSG00000138190.17. [Q8TAG9-1]
DR Ensembl; ENST00000371552.8; ENSP00000360607.4; ENSG00000138190.17. [Q8TAG9-2]
DR Ensembl; ENST00000671701.1; ENSP00000500529.1; ENSG00000138190.17. [Q8TAG9-2]
DR Ensembl; ENST00000672817.1; ENSP00000500468.1; ENSG00000138190.17. [Q8TAG9-2]
DR GeneID; 54536; -.
DR KEGG; hsa:54536; -.
DR MANE-Select; ENST00000260762.10; ENSP00000260762.6; NM_019053.6; NP_061926.3.
DR UCSC; uc001kie.4; human. [Q8TAG9-1]
DR CTD; 54536; -.
DR DisGeNET; 54536; -.
DR GeneCards; EXOC6; -.
DR HGNC; HGNC:23196; EXOC6.
DR HPA; ENSG00000138190; Tissue enriched (retina).
DR MIM; 609672; gene.
DR neXtProt; NX_Q8TAG9; -.
DR OpenTargets; ENSG00000138190; -.
DR PharmGKB; PA134908462; -.
DR VEuPathDB; HostDB:ENSG00000138190; -.
DR eggNOG; KOG2176; Eukaryota.
DR GeneTree; ENSGT00390000005739; -.
DR HOGENOM; CLU_009437_0_0_1; -.
DR InParanoid; Q8TAG9; -.
DR OMA; RDHYNEV; -.
DR OrthoDB; 185623at2759; -.
DR PhylomeDB; Q8TAG9; -.
DR TreeFam; TF315199; -.
DR PathwayCommons; Q8TAG9; -.
DR Reactome; R-HSA-1445148; Translocation of SLC2A4 (GLUT4) to the plasma membrane.
DR Reactome; R-HSA-264876; Insulin processing.
DR Reactome; R-HSA-5620916; VxPx cargo-targeting to cilium.
DR SignaLink; Q8TAG9; -.
DR BioGRID-ORCS; 54536; 20 hits in 1078 CRISPR screens.
DR ChiTaRS; EXOC6; human.
DR GenomeRNAi; 54536; -.
DR Pharos; Q8TAG9; Tbio.
DR PRO; PR:Q8TAG9; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q8TAG9; protein.
DR Bgee; ENSG00000138190; Expressed in deltoid and 189 other tissues.
DR ExpressionAtlas; Q8TAG9; baseline and differential.
DR Genevisible; Q8TAG9; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0000145; C:exocyst; IBA:GO_Central.
DR GO; GO:0090543; C:Flemming body; IEA:UniProtKB-SubCell.
DR GO; GO:0030426; C:growth cone; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0090148; P:membrane fission; IC:ComplexPortal.
DR GO; GO:0000281; P:mitotic cytokinesis; IC:ComplexPortal.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; IC:ComplexPortal.
DR GO; GO:0090522; P:vesicle tethering involved in exocytosis; IC:ComplexPortal.
DR Gene3D; 1.10.357.30; -; 1.
DR Gene3D; 1.20.58.670; -; 1.
DR InterPro; IPR007225; EXOC6/Sec15.
DR InterPro; IPR042045; EXOC6/Sec15_C_dom1.
DR InterPro; IPR042044; EXOC6PINT-1/Sec15/Tip20_C_dom2.
DR InterPro; IPR046361; Sec15_C.
DR PANTHER; PTHR12702; PTHR12702; 1.
DR Pfam; PF04091; Sec15; 1.
DR PIRSF; PIRSF025007; Sec15; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cytoplasm; Exocytosis;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..804
FT /note="Exocyst complex component 6"
FT /id="PRO_0000118951"
FT VAR_SEQ 2..31
FT /note="AENSESLGTVPEHERILQEIESTDTACVGP -> LEEETDQTYENVLAEIQS
FT FELPVEA (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047160"
FT VARIANT 396
FT /note="T -> I (in dbSNP:rs1326331)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_044522"
FT VARIANT 523
FT /note="L -> V (in dbSNP:rs11187225)"
FT /id="VAR_044523"
FT VARIANT 578
FT /note="T -> I (in dbSNP:rs35647717)"
FT /id="VAR_044524"
FT CONFLICT 776
FT /note="R -> G (in Ref. 3; AAH28395)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 804 AA; 93722 MW; 413251A5BA6543C4 CRC64;
MAENSESLGT VPEHERILQE IESTDTACVG PTLRSVYDDQ PNAHKKFMEK LDACIRNHDK
EIEKMCNFHH QGFVDAITEL LKVRTDAEKL KVQVTDTNRR FQDAGKEVIV HTEDIIRCRI
QQRNITTVVE KLQLCLPVLE MYSKLKEQMS AKRYYSALKT MEQLENVYFP WVSQYRFCQL
MIENLPKLRE DIKEISMSDL KDFLESIRKH SDKIGETAMK QAQHQKTFSV SLQKQNKMKF
GKNMYINRDR IPEERNETVL KHSLEEEDEN EEEILTVQDL VDFSPVYRCL HIYSVLGDEE
TFENYYRKQR KKQARLVLQP QSNMHETVDG YRRYFTQIVG FFVVEDHILH VTQGLVTRAY
TDELWNMALS KIIAVLRAHS SYCTDPDLVL ELKNLTVIFA DTLQGYGFPV NRLFDLLFEI
RDQYNETLLK KWAGVFRDIF EEDNYSPIPV VNEEEYKIVI SKFPFQDPDL EKQSFPKKFP
MSQSVPHIYI QVKEFIYASL KFSESLHRSS TEIDDMLRKS TNLLLTRTLS SCLLNLIRKP
HIGLTELVQI IINTTHLEQA CKYLEDFITN ITNISQETVH TTRLYGLSTF KDARHAAEGE
IYTKLNQKID EFVQLADYDW TMSEPDGRAS GYLMDLINFL RSIFQVFTHL PGKVAQTACM
SACQHLSTSL MQMLLDSELK QISMGAVQQF NLDVIQCELF ASSEPVPGFQ GDTLQLAFID
LRQLLDLFMV WDWSTYLADY GQPASKYLRV NPNTALTLLE KMKDTSKKNN IFAQFRKNDR
DKQKLIETVV KQLRSLVNGM SQHM