EXOC7_DROME
ID EXOC7_DROME Reviewed; 693 AA.
AC Q9VSJ8; Q8T0E9;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Exocyst complex component 7;
DE AltName: Full=Exocyst complex component Exo70;
GN Name=Exo70 {ECO:0000312|FlyBase:FBgn0266667};
GN ORFNames=CG7127 {ECO:0000312|FlyBase:FBgn0266667};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAL39511.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND SUBUNIT.
RX PubMed=10908590; DOI=10.1083/jcb.150.2.f77;
RA Littleton J.T.;
RT "A genomic analysis of membrane trafficking and neurotransmitter release in
RT Drosophila.";
RL J. Cell Biol. 150:F77-F82(2000).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-236, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required for exocytosis. Thought to function in intracellular
CC vesicle targeting and docking before SNARE complex formation.
CC {ECO:0000303|PubMed:10908590}.
CC -!- SUBUNIT: The exocyst complex is composed of Sec3/Exoc1, Sec5/Exoc2,
CC Sec6/Exoc3, Sec8/Exoc4, Sec10/Exoc5, Sec15/Exoc6, Exo70/Exoc7 and
CC Exo84/Exoc8. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the EXO70 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50421.2; -; Genomic_DNA.
DR EMBL; AY069366; AAL39511.1; -; mRNA.
DR RefSeq; NP_648222.3; NM_139965.3.
DR AlphaFoldDB; Q9VSJ8; -.
DR SMR; Q9VSJ8; -.
DR BioGRID; 64372; 35.
DR DIP; DIP-23867N; -.
DR IntAct; Q9VSJ8; 2.
DR STRING; 7227.FBpp0076339; -.
DR iPTMnet; Q9VSJ8; -.
DR PaxDb; Q9VSJ8; -.
DR PRIDE; Q9VSJ8; -.
DR DNASU; 38959; -.
DR EnsemblMetazoa; FBtr0076612; FBpp0076339; FBgn0266667.
DR GeneID; 38959; -.
DR KEGG; dme:Dmel_CG7127; -.
DR UCSC; CG7127-RA; d. melanogaster.
DR CTD; 38959; -.
DR FlyBase; FBgn0266667; Exo70.
DR VEuPathDB; VectorBase:FBgn0266667; -.
DR eggNOG; KOG2344; Eukaryota.
DR GeneTree; ENSGT00390000003595; -.
DR HOGENOM; CLU_010236_4_0_1; -.
DR InParanoid; Q9VSJ8; -.
DR OMA; DYFRHNN; -.
DR OrthoDB; 410847at2759; -.
DR PhylomeDB; Q9VSJ8; -.
DR Reactome; R-DME-264876; Insulin processing.
DR Reactome; R-DME-5620916; VxPx cargo-targeting to cilium.
DR SignaLink; Q9VSJ8; -.
DR BioGRID-ORCS; 38959; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Exo70; fly.
DR GenomeRNAi; 38959; -.
DR PRO; PR:Q9VSJ8; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0266667; Expressed in second segment of antenna (Drosophila) and 25 other tissues.
DR ExpressionAtlas; Q9VSJ8; baseline and differential.
DR Genevisible; Q9VSJ8; DM.
DR GO; GO:0000145; C:exocyst; ISS:FlyBase.
DR GO; GO:0043195; C:terminal bouton; IDA:FlyBase.
DR GO; GO:0008013; F:beta-catenin binding; IPI:FlyBase.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; ISS:FlyBase.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0007269; P:neurotransmitter secretion; NAS:FlyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0051124; P:synaptic assembly at neuromuscular junction; IMP:FlyBase.
DR GO; GO:0016081; P:synaptic vesicle docking; NAS:FlyBase.
DR GO; GO:0016080; P:synaptic vesicle targeting; NAS:FlyBase.
DR GO; GO:0016192; P:vesicle-mediated transport; ISS:FlyBase.
DR InterPro; IPR016159; Cullin_repeat-like_dom_sf.
DR InterPro; IPR004140; Exo70.
DR InterPro; IPR046364; Exo70_C.
DR PANTHER; PTHR12542; PTHR12542; 1.
DR Pfam; PF03081; Exo70; 1.
DR SUPFAM; SSF74788; SSF74788; 1.
PE 1: Evidence at protein level;
KW Exocytosis; Phosphoprotein; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..693
FT /note="Exocyst complex component 7"
FT /id="PRO_0000118963"
FT REGION 236..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..253
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 236
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 543
FT /note="L -> F (in Ref. 3; AAL39511)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 693 AA; 80026 MW; 53FA91C081A39851 CRC64;
MNNLDSSLQA HNKLEKEATN LALLKDRVDK YHDLSTQMSS ILTIFEKRLG NLEQTILPVY
QETEQLQKRQ QNLEATLNCL ESVLSHYDVS QEVCQLIHQG PVEGNISVFL DALAKLRDAN
DYFRHNNSQS VELENVTSLF NTGCEGLSQH YSMLLKKHSA PLKPVELLDL IYIEDDSSDE
YTSFRQLSQT TREELYTISH WLEQNLREYT NIYATERGEV VLRSLQLLKD HQKSNSWGHE
ALRPRHSGRQ TEPKKTTSAR LQQIFEKKAN KLYLRATQTI EQSTGFSIKK ASSHSDHLTS
EDLMDGDQEL DKYLVMLLGL QRLLNWERAI MIDIIPQSKH NEVFATLAYN AIDLVVKDAE
AITQRILRCI SRKEWTSALG IFSALKRVIL LQPDIDRTYD PAQREQLKKV LKKLQHTGAK
ALEHFLDVVK GESSTNIVGQ SNVPKDATVH ELTSNTIWFI EHLYDHFDVI GSILAQDVLY
STQLDTILMK KALPVEERNK ALLAIYIKKA LAELNLSIMN KCEQYNDQAT KHLFRLNNIH
YILKSLQRSN LIDLVTLAEP ECEHSYMEMI RELKASYQKT WSKMLVGIYS LDELPKPVAG
KVKDKDRSVL KERFSNFNKD FEEACKIQRG ISIPDVILRE GIKRDNVEHI LPIYNRFYEI
YSGVHFSKNP DKYVKYRQHE INAMLSKLFD DSA
