ID   EXOD_BPT4               Reviewed;         227 AA.
AC   P04536;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   23-FEB-2022, entry version 89.
DE   RecName: Full=Exodeoxyribonuclease;
DE            EC=3.1.11.1;
DE   AltName: Full=3'-5' exonuclease;
GN   Name=dexA;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3553862; DOI=10.1007/bf00326532;
RA   Gauss P., Gayle M., Winter R.B., Gold L.;
RT   "The bacteriophage T4 dexA gene: sequence and analysis of a gene
RT   conditionally required for DNA replication.";
RL   Mol. Gen. Genet. 206:24-34(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   FUNCTION.
RX   PubMed=3056918; DOI=10.1128/jb.170.12.5830-5836.1988;
RA   Gruber H., Kern G., Gauss P., Gold L.;
RT   "Effect of DNA sequence and structure on nuclease activity of the DexA
RT   protein of bacteriophage T4.";
RL   J. Bacteriol. 170:5830-5836(1988).
RN   [4]
RP   FUNCTION IN INTRON HOMING.
RX   PubMed=10581261; DOI=10.1093/genetics/153.4.1501;
RA   Huang Y.J., Parker M.M., Belfort M.;
RT   "Role of exonucleolytic degradation in group I intron homing in phage T4.";
RL   Genetics 153:1501-1512(1999).
CC   -!- FUNCTION: 3'-5' exonuclease that preferentially uses ssDNA as
CC       substrate. Plays a role in group I intron homing. May play a role in
CC       the final step of host DNA degradation, by scavenging DNA into
CC       mononucleotides. {ECO:0000269|PubMed:10581261,
CC       ECO:0000269|PubMed:3056918}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC         nucleoside 5'-phosphates.; EC=3.1.11.1;
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DR   EMBL; X04834; CAA28536.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42564.1; -; Genomic_DNA.
DR   PIR; B32338; ZXBPB4.
DR   RefSeq; NP_049629.1; NC_000866.4.
DR   PRIDE; P04536; -.
DR   GeneID; 1258605; -.
DR   KEGG; vg:1258605; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0008852; F:exodeoxyribonuclease I activity; IEA:UniProtKB-EC.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR033390; Rv2179c-like.
DR   Pfam; PF16473; DUF5051; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   1: Evidence at protein level;
KW   Exonuclease; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..227
FT                   /note="Exodeoxyribonuclease"
FT                   /id="PRO_0000164940"
SQ   SEQUENCE   227 AA;  25964 MW;  7B27FFF3E6CC8E0A CRC64;
     MFDFIIDFET MGSGEKAAVI DLAVIAFDPN PEVVETFDEL VSRGIKIKFD LKSQKGHRLF
     TKSTIEWWKN QSPEARKNIA PSDEDVSTID GIAKFNDYIN AHNIDPWKSQ GWCRGMSFDF
     PILVDLIRDI QRLNGVSENE LDTFKLEPCK FWNQRDIRTR IEALLLVRDM TTCPLPKGTL
     DGFVAHDSIH DCAKDILMMK YALRYAMGLE DAPSEEECDP LSLPTKR