EXOH_RHIME
ID EXOH_RHIME Reviewed; 370 AA.
AC P33692;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Succinoglycan biosynthesis protein ExoH;
GN Name=exoH; OrderedLocusNames=RB1079; ORFNames=SMb20954;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymB (megaplasmid 2).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT by Rhizobium meliloti.";
RL J. Bacteriol. 175:7033-7044(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RCR2011 / SU47;
RX PubMed=8479421; DOI=10.1007/bf00279541;
RA Becker A., Kleickmann A., Arnold W., Puehler A.;
RT "Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows
RT homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles
RT membrane proteins.";
RL Mol. Gen. Genet. 238:145-154(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481431; DOI=10.1073/pnas.161294698;
RA Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT endosymbiont Sinorhizobium meliloti.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Required for the succinyl modification of the seventh sugar
CC (glucose) of the octasaccharide subunit of succinoglycan (EPS I).
CC -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the acyltransferase 3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA78926.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L20758; AAA16049.1; -; Unassigned_DNA.
DR EMBL; Z17219; CAA78926.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL591985; CAC49479.1; -; Genomic_DNA.
DR PIR; G95976; G95976.
DR PIR; S34803; S34803.
DR RefSeq; NP_437619.1; NC_003078.1.
DR RefSeq; WP_010975916.1; NC_003078.1.
DR AlphaFoldDB; P33692; -.
DR STRING; 266834.SM_b20954; -.
DR EnsemblBacteria; CAC49479; CAC49479; SM_b20954.
DR GeneID; 61601034; -.
DR KEGG; sme:SM_b20954; -.
DR PATRIC; fig|266834.11.peg.6007; -.
DR eggNOG; COG1835; Bacteria.
DR HOGENOM; CLU_054154_1_0_5; -.
DR OMA; WIFCAHY; -.
DR BioCyc; MetaCyc:SM_B20954-MON; -.
DR UniPathway; UPA00631; -.
DR Proteomes; UP000001976; Plasmid pSymB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR002656; Acyl_transf_3_dom.
DR Pfam; PF01757; Acyl_transf_3; 1.
PE 3: Inferred from homology;
KW Cell membrane; Exopolysaccharide synthesis; Membrane; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..370
FT /note="Succinoglycan biosynthesis protein ExoH"
FT /id="PRO_0000208070"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 350..370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 218
FT /note="A -> E (in Ref. 1; AAA16049)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="R -> G (in Ref. 1; AAA16049)"
FT /evidence="ECO:0000305"
FT CONFLICT 337
FT /note="P -> A (in Ref. 2; CAA78926)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 41213 MW; 40DB4C60C03F3DA0 CRC64;
MDANVSARIN LMRILLISGI VFVHVPYNPQ WSPFLGNYGM LDWLRVFLGE SLFRIGVPCL
SAISGYLLFR RGLADFDYWK TLKTKARTVL LPFLIWSGSF FVVVYAIQRQ GLGFGYLPDT
INATPRQWLS MAFAVEATPV NLPLYFLRDL MLCILLSPLL ALLVSRYPRV TLLALLAYAI
LPLPNGIFLK KSILFGFSAG IYASLHGVNI KMLDRFAAPI AAGFLAIAVV IAVGLYYTGP
DFPLWLDMAL RLTSIAGIIG SWAISELLVR TRFGEKLGRG SGLSFWIFCG HYPLLVLFWM
IWNRTGLSYY PLFYFTAPFI AIAILVASHN LVRRLAPDLL AVLTGSRTGA KRMATQPPQG
AQAGYSPQQR
