EXOL_RHIME
ID EXOL_RHIME Reviewed; 403 AA.
AC P33694;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 24-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Succinoglycan biosynthesis protein ExoL;
DE EC=2.-.-.-;
GN Name=exoL; OrderedLocusNames=RB1081; ORFNames=SMb20956;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymB (megaplasmid 2).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT by Rhizobium meliloti.";
RL J. Bacteriol. 175:7033-7044(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RCR2011 / SU47;
RX PubMed=8479421; DOI=10.1007/bf00279541;
RA Becker A., Kleickmann A., Arnold W., Puehler A.;
RT "Analysis of the Rhizobium meliloti exoH/exoK/exoL fragment: ExoK shows
RT homology to excreted endo-beta-1,3-1,4-glucanases and ExoH resembles
RT membrane proteins.";
RL Mol. Gen. Genet. 238:145-154(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481431; DOI=10.1073/pnas.161294698;
RA Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT endosymbiont Sinorhizobium meliloti.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 287-403.
RC STRAIN=RCR2011 / SU47;
RX PubMed=8246891; DOI=10.1007/bf00284690;
RA Becker A., Kleickmann A., Keller M., Arnold W., Puehler A.;
RT "Identification and analysis of the Rhizobium meliloti exoAMONP genes
RT involved in exopolysaccharide biosynthesis and mapping of promoters located
RT on the exoHKLAMONP fragment.";
RL Mol. Gen. Genet. 241:367-379(1993).
CC -!- FUNCTION: Essential for succinoglycan (EPS I) synthesis and nodule
CC infection. Glycosyltransferase needed for the addition of the third
CC sugar (glucose), catalyzes the formation of a beta-1,4 linkage between
CC the second and third sugars.
CC -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L20758; AAA16047.1; -; Unassigned_DNA.
DR EMBL; Z17219; CAA78928.1; -; Genomic_DNA.
DR EMBL; AL591985; CAC49481.1; -; Genomic_DNA.
DR EMBL; Z22636; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A95977; A95977.
DR PIR; S34805; S34805.
DR RefSeq; NP_437621.1; NC_003078.1.
DR RefSeq; WP_010975918.1; NC_003078.1.
DR AlphaFoldDB; P33694; -.
DR SMR; P33694; -.
DR STRING; 266834.SM_b20956; -.
DR EnsemblBacteria; CAC49481; CAC49481; SM_b20956.
DR GeneID; 61601036; -.
DR KEGG; sme:SM_b20956; -.
DR PATRIC; fig|266834.11.peg.6009; -.
DR eggNOG; COG0438; Bacteria.
DR HOGENOM; CLU_061763_0_0_5; -.
DR OMA; PWRIGWF; -.
DR BioCyc; MetaCyc:SM_B20956-MON; -.
DR UniPathway; UPA00631; -.
DR Proteomes; UP000001976; Plasmid pSymB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
PE 4: Predicted;
KW Cytoplasm; Exopolysaccharide synthesis; Glycosyltransferase; Plasmid;
KW Reference proteome; Transferase.
FT CHAIN 1..403
FT /note="Succinoglycan biosynthesis protein ExoL"
FT /id="PRO_0000087124"
FT CONFLICT 123..124
FT /note="ML -> IV (in Ref. 1; AAA16047)"
FT /evidence="ECO:0000305"
FT CONFLICT 379
FT /note="L -> V (in Ref. 1; AAA16047)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 403 AA; 44492 MW; FC889DBCA1CE2241 CRC64;
MLQILYLAQD LADPAVRRRT LTLVAGGARV TLAGFRRGDN PLAAIDGVEP IELGTTADGR
FAQRIGAVAR ACLSLQRQLG HVRKPDVIIA RNLEMLAVAR RAVAFFGGTV PIVYECLDIH
RLMLRKDIVG RMLRAAESQL GKDARLLITS SPAFIEHYFR PLSGIGAPPM LLENKVLEID
GTVERRTASP AKSPPPGAPW KIGWFGALRC RRSLALLAEF SRKMEGRFEI VLRGRPAYSE
FDDFDGFVRN EPFMRFEGAY RNPEDLAEIY GEVHFTWAID FFEEGQNSAW LLPNRLYEGC
RHGRIPIAMK GTETARFLSV RSIGLVLEGA DVESLATVLG PLTPNCYADA AERISRCNPG
SWVFDRTDCE ALVRQLATLT LQAPQTVPVV AMAGSSHKEG GFL
