EXON_RHIME
ID EXON_RHIME Reviewed; 301 AA.
AC P33696;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 24-OCT-2001, sequence version 2.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=UTP--glucose-1-phosphate uridylyltransferase;
DE EC=2.7.7.9;
DE AltName: Full=Alpha-D-glucosyl-1-phosphate uridylyltransferase;
DE AltName: Full=UDP-glucose pyrophosphorylase;
DE Short=UDPGP;
DE AltName: Full=Uridine diphosphoglucose pyrophosphorylase;
GN Name=exoN; OrderedLocusNames=RB1085; ORFNames=SMb20960;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymB (megaplasmid 2).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT by Rhizobium meliloti.";
RL J. Bacteriol. 175:7033-7044(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226646; DOI=10.1128/jb.175.21.7045-7055.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Genes needed for the modification, polymerization, export, and processing
RT of succinoglycan by Rhizobium meliloti: a model for succinoglycan
RT biosynthesis.";
RL J. Bacteriol. 175:7045-7055(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RCR2011 / SU47;
RX PubMed=8246891; DOI=10.1007/bf00284690;
RA Becker A., Kleickmann A., Keller M., Arnold W., Puehler A.;
RT "Identification and analysis of the Rhizobium meliloti exoAMONP genes
RT involved in exopolysaccharide biosynthesis and mapping of promoters located
RT on the exoHKLAMONP fragment.";
RL Mol. Gen. Genet. 241:367-379(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481431; DOI=10.1073/pnas.161294698;
RA Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT endosymbiont Sinorhizobium meliloti.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-
CC alpha-D-glucose; Xref=Rhea:RHEA:19889, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:58601,
CC ChEBI:CHEBI:58885; EC=2.7.7.9;
CC -!- PATHWAY: Carbohydrate metabolism; nucleotide-sugar metabolism.
CC -!- SIMILARITY: Belongs to the UDPGP type 2 family. {ECO:0000305}.
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DR EMBL; L20758; AAA16043.1; -; Unassigned_DNA.
DR EMBL; Z22636; CAA80348.1; -; Genomic_DNA.
DR EMBL; AL591985; CAC49485.1; -; Genomic_DNA.
DR PIR; D49349; D49349.
DR PIR; E95977; E95977.
DR RefSeq; NP_437625.1; NC_003078.1.
DR RefSeq; WP_010975921.1; NC_003078.1.
DR AlphaFoldDB; P33696; -.
DR SMR; P33696; -.
DR STRING; 266834.SM_b20960; -.
DR EnsemblBacteria; CAC49485; CAC49485; SM_b20960.
DR GeneID; 25012635; -.
DR GeneID; 61601040; -.
DR KEGG; sme:SM_b20960; -.
DR PATRIC; fig|266834.11.peg.6013; -.
DR eggNOG; COG1210; Bacteria.
DR HOGENOM; CLU_029499_1_2_5; -.
DR OMA; KRYDVGN; -.
DR UniPathway; UPA00215; -.
DR PRO; PR:P33696; -.
DR Proteomes; UP000001976; Plasmid pSymB.
DR GO; GO:0003983; F:UTP:glucose-1-phosphate uridylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006011; P:UDP-glucose metabolic process; IEA:InterPro.
DR CDD; cd02541; UGPase_prokaryotic; 1.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR005771; GalU_uridylyltTrfase_bac/arc.
DR InterPro; IPR005835; NTP_transferase_dom.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR43197; PTHR43197; 1.
DR Pfam; PF00483; NTP_transferase; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Exopolysaccharide synthesis; Nucleotidyltransferase; Plasmid;
KW Reference proteome; Transferase.
FT CHAIN 1..301
FT /note="UTP--glucose-1-phosphate uridylyltransferase"
FT /id="PRO_0000201347"
FT CONFLICT 275..301
FT /note="ARADIGDIVFESVRDMVLSHESRIRAA -> LVRTSAISFSNPSATWFCLTK
FT AASGRHKRRLPAVLRDCRNKDRRRR (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 301 AA; 32584 MW; 03AE86FB658E9532 CRC64;
MDRVRTVRKA VIPVAGNGTR FLPATKAVPK EMLTIVDRPV VQYAVDEARQ AGIEHIVFVT
SRNKQVIEDH FDDAPELISS LSRSGKSAQI SELEAMLPAA GSVSFTRQQA PLGLGHAVWC
ARDLIGDEPF ALLLPDMVSF GARGCVAGLM DLYHEVGGNV VGVEQCAPEE ASKYGIVGKG
ETVRHGFSVT EMVEKPAAGK APSNYYLNGR YILQPEIFSI LAHQTRGAGN EIQLTDGMLT
LSQSQSFHAH PYEGRTFDCG SKQGFIEANV AFALARADIG DIVFESVRDM VLSHESRIRA
A
