ID   EXOO_RHIME              Reviewed;         348 AA.
AC   P33697;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   24-OCT-2001, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Succinoglycan biosynthesis protein ExoO;
DE            EC=2.4.-.-;
GN   Name=exoO; OrderedLocusNames=RB1084; ORFNames=SMb20959;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA   Glucksmann M.A., Reuber T.L., Walker G.C.;
RT   "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT   by Rhizobium meliloti.";
RL   J. Bacteriol. 175:7033-7044(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RCR2011 / SU47;
RX   PubMed=8246891; DOI=10.1007/bf00284690;
RA   Becker A., Kleickmann A., Keller M., Arnold W., Puehler A.;
RT   "Identification and analysis of the Rhizobium meliloti exoAMONP genes
RT   involved in exopolysaccharide biosynthesis and mapping of promoters located
RT   on the exoHKLAMONP fragment.";
RL   Mol. Gen. Genet. 241:367-379(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Glycosyltransferase required for the synthesis of
CC       succinoglycan (EPS I). Needed for the addition of the fifth sugar
CC       (glucose), catalyzes the formation of a beta-1,6 linkage between the
CC       fourth and fifth sugar.
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA16044.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; L20758; AAA16044.1; ALT_FRAME; Unassigned_DNA.
DR   EMBL; Z22636; CAA80347.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49484.1; -; Genomic_DNA.
DR   PIR; C49348; C49348.
DR   PIR; D95977; D95977.
DR   PIR; S39958; S39958.
DR   RefSeq; NP_437624.1; NC_003078.1.
DR   RefSeq; WP_010975920.1; NC_003078.1.
DR   AlphaFoldDB; P33697; -.
DR   SMR; P33697; -.
DR   STRING; 266834.SM_b20959; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; CAC49484; CAC49484; SM_b20959.
DR   GeneID; 61601039; -.
DR   KEGG; sme:SM_b20959; -.
DR   PATRIC; fig|266834.11.peg.6012; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_025996_0_1_5; -.
DR   OMA; VLMANYR; -.
DR   BioCyc; MetaCyc:SM_B20959-MON; -.
DR   UniPathway; UPA00631; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exopolysaccharide synthesis; Glycosyltransferase; Plasmid;
KW   Reference proteome; Transferase.
FT   CHAIN           1..348
FT                   /note="Succinoglycan biosynthesis protein ExoO"
FT                   /id="PRO_0000059183"
FT   REGION          322..348
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   348 AA;  38132 MW;  770B43782F785579 CRC64;
     MNPPAIDKVP DVTFVVAAYN SADTIVRAIE SALAQEGVTV EVVVVDDCSA DATPALVAAI
     PDPRVRLIAL DRNRGPGGAR NAGIGAARGR WIAVLDSDDT VRPDRLRRMI ERADAAGAQI
     AVDNLDVVSL DGRSLRMFSE AELARLPQLT LPAFIESNVL FRSEHNFGYM KPIFERRFLE
     NQQLRFDEAL RIGEDYILLA SALACGGRCA VEPSAGYIYH IREGSISRVL RLDHIDAMIA
     ADEAFLRRYA LDGLAQKMQH RRMRGFREAR SFLVLVEQLK KRSLAGALKT ALADPFALRH
     LSMPIAARLR RLAARFVHPS SHSAPRAAPV TAAAERSPLG NDPRISKG