EXOP_RHIME
ID EXOP_RHIME Reviewed; 786 AA.
AC P33698;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Succinoglycan biosynthesis transport protein ExoP;
GN Name=exoP; OrderedLocusNames=RB1086; ORFNames=SMb20961;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymB (megaplasmid 2).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT by Rhizobium meliloti.";
RL J. Bacteriol. 175:7033-7044(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226646; DOI=10.1128/jb.175.21.7045-7055.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Genes needed for the modification, polymerization, export, and processing
RT of succinoglycan by Rhizobium meliloti: a model for succinoglycan
RT biosynthesis.";
RL J. Bacteriol. 175:7045-7055(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RCR2011 / SU47;
RX PubMed=8246891; DOI=10.1007/bf00284690;
RA Becker A., Kleickmann A., Keller M., Arnold W., Puehler A.;
RT "Identification and analysis of the Rhizobium meliloti exoAMONP genes
RT involved in exopolysaccharide biosynthesis and mapping of promoters located
RT on the exoHKLAMONP fragment.";
RL Mol. Gen. Genet. 241:367-379(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481431; DOI=10.1073/pnas.161294698;
RA Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT endosymbiont Sinorhizobium meliloti.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: To B.solanacearum EpsB. {ECO:0000305}.
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DR EMBL; L20758; AAA16042.1; -; Unassigned_DNA.
DR EMBL; Z22636; CAA80349.1; -; Genomic_DNA.
DR EMBL; AL591985; CAC49486.1; -; Genomic_DNA.
DR PIR; F95977; F95977.
DR PIR; S37031; S37031.
DR RefSeq; NP_437626.1; NC_003078.1.
DR RefSeq; WP_010975922.1; NC_003078.1.
DR AlphaFoldDB; P33698; -.
DR SMR; P33698; -.
DR STRING; 266834.SM_b20961; -.
DR TCDB; 8.A.3.1.1; the cytoplasmic membrane-periplasmic auxiliary-1 (mpa1) protein with cytoplasmic (c) domain (mpa1-c or mpa1+c) family.
DR EnsemblBacteria; CAC49486; CAC49486; SM_b20961.
DR GeneID; 61601041; -.
DR KEGG; sme:SM_b20961; -.
DR PATRIC; fig|266834.11.peg.6015; -.
DR eggNOG; COG0489; Bacteria.
DR eggNOG; COG3206; Bacteria.
DR HOGENOM; CLU_009912_2_0_5; -.
DR OMA; QQIYIQL; -.
DR BioCyc; MetaCyc:SM_B20961-MON; -.
DR UniPathway; UPA00631; -.
DR Proteomes; UP000001976; Plasmid pSymB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR InterPro; IPR005700; EPS_ExoP-like.
DR InterPro; IPR005702; EPS_synthesis.
DR InterPro; IPR032807; GNVR.
DR InterPro; IPR003856; LPS_length_determ_N_term.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01656; CbiA; 1.
DR Pfam; PF13807; GNVR; 1.
DR Pfam; PF02706; Wzz; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01007; eps_fam; 1.
DR TIGRFAMs; TIGR01005; eps_transp_fam; 1.
PE 4: Predicted;
KW ATP-binding; Cell membrane; Exopolysaccharide synthesis; Membrane;
KW Nucleotide-binding; Plasmid; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..786
FT /note="Succinoglycan biosynthesis transport protein ExoP"
FT /id="PRO_0000087125"
FT TOPO_DOM 1..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..689
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 690..711
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 712..786
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT BINDING 583..590
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 786 AA; 86124 MW; E19E771E31F2030C CRC64;
MNRTAPMKQR SVPLSSIMPS EEQSDGFIDL DRLVAAVFRR ARLVAAFVVL FIALGAAYLL
FATPYYTSMT QILLDENLSK YAEEEPTPVN SQMLDTQIAS AVEILKSGEL ALRVVDKLKL
SENDTILNPP RSPVDMVKEW LKTATGLFSG GPDVTEEAAR NGRRQKAAAI IQQSLAVERV
SRSSVVAVAF RSSDPLLAAT IARGYASAYL TDQLNANFEA TERASVWLQE RLTDLQQRSQ
AAALEVAHFR AENGLTAARG ELMSEQQMAD LNSQLIVAQA DTASASARYD QYKSIVDQGP
ENAVKNATIS SKEGDNSVIR DLRTRYLTVG KREREVSDNF GADHPQAVSL RAEQEDVARQ
IYQELQQLTA SYKNEYEVAQ SREESLRKSI QGIAGKTSDA SEQLVQLREL EQKAAALKTL
YESYLGRYEQ ATQQQSFPIA KARVISEAGV PVSPSSPKKT MTLALSAVLG MMVGGAYAAF
LEFRERTFRL EGNIRSILGH RSLGYVPLLG TRMKKKAQLV HAHFGSVKRH DEAVDDTMPF
QRLSRIVVDA PRSTFAETFR NAKLACDQML AGSESRVIAI ASALPDEGKS IIAANFAALL
AASGKRTLLI DADIRKPGLT QMITPAPRTG LVETLIGEAS WPAGIKVDQR TKLAILPAGG
ASHQRHQSNE LLASPAMANL IENARNAFDY VVVDLAALAP VVDAKAFAPL ADGILFVVEW
GRTPSRLVRD LLHSEPLINS KVLGVILNKT DMNELGKYSD FDGAEKYRHR YGKYYVENTI
TENTAA
