EXOS3_BOVIN
ID EXOS3_BOVIN Reviewed; 275 AA.
AC Q3T0E1;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Exosome complex component RRP40;
DE AltName: Full=Exosome component 3;
DE AltName: Full=Ribosomal RNA-processing protein 40;
GN Name=EXOSC3; Synonyms=RRP40;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Non-catalytic component of the RNA exosome complex which has
CC 3'->5' exoribonuclease activity and participates in a multitude of
CC cellular RNA processing and degradation events. In the nucleus, the RNA
CC exosome complex is involved in proper maturation of stable RNA species
CC such as rRNA, snRNA and snoRNA, in the elimination of RNA processing
CC by-products and non-coding 'pervasive' transcripts, such as antisense
CC RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs
CC with processing defects, thereby limiting or excluding their export to
CC the cytoplasm. The RNA exosome may be involved in Ig class switch
CC recombination (CSR) and/or Ig variable region somatic hypermutation
CC (SHM) by targeting AICDA deamination activity to transcribed dsDNA
CC substrates. In the cytoplasm, the RNA exosome complex is involved in
CC general mRNA turnover and specifically degrades inherently unstable
CC mRNAs containing AU-rich elements (AREs) within their 3' untranslated
CC regions, and in RNA surveillance pathways, preventing translation of
CC aberrant mRNAs. It seems to be involved in degradation of histone mRNA.
CC The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9)
CC is proposed to play a pivotal role in the binding and presentation of
CC RNA for ribonucleolysis, and to serve as a scaffold for the association
CC with catalytic subunits and accessory proteins or complexes. EXOSC3 as
CC peripheral part of the Exo-9 complex stabilizes the hexameric ring of
CC RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5 (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA exosome complex. Specifically part of the
CC catalytically inactive RNA exosome core (Exo-9) complex which is
CC believed to associate with catalytic subunits EXOSC10, and DIS3 or
CC DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms.
CC Exo-9 is formed by a hexameric ring of RNase PH domain-containing
CC subunits specifically containing the heterodimers EXOSC4-EXOSC9,
CC EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and peripheral S1 domain-containing
CC components EXOSC1, EXOSC2 and EXOSC3 located on the top of the ring
CC structure. Interacts with GTPBP1 (By similarity). Interacts with
CC ZC3HAV1 (By similarity). Interacts with DDX17 only in the presence of
CC ZC3HAV1 in an RNA-independent manner (By similarity). Interacts with
CC DHX36; this interaction occurs in a RNase-insensitive manner (By
CC similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q9NQT5}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus, nucleolus
CC {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RRP40 family. {ECO:0000305}.
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DR EMBL; BC102436; AAI02437.1; -; mRNA.
DR RefSeq; NP_001030474.1; NM_001035397.2.
DR AlphaFoldDB; Q3T0E1; -.
DR SMR; Q3T0E1; -.
DR STRING; 9913.ENSBTAP00000025185; -.
DR PaxDb; Q3T0E1; -.
DR PRIDE; Q3T0E1; -.
DR Ensembl; ENSBTAT00000025185; ENSBTAP00000025185; ENSBTAG00000018925.
DR GeneID; 533245; -.
DR KEGG; bta:533245; -.
DR CTD; 51010; -.
DR VEuPathDB; HostDB:ENSBTAG00000018925; -.
DR VGNC; VGNC:28658; EXOSC3.
DR eggNOG; KOG1004; Eukaryota.
DR GeneTree; ENSGT00940000153596; -.
DR HOGENOM; CLU_069847_1_0_1; -.
DR InParanoid; Q3T0E1; -.
DR OMA; SYMAFPN; -.
DR OrthoDB; 1284731at2759; -.
DR TreeFam; TF314927; -.
DR Proteomes; UP000009136; Chromosome 8.
DR Bgee; ENSBTAG00000018925; Expressed in oocyte and 105 other tissues.
DR GO; GO:0000177; C:cytoplasmic exosome (RNase complex); IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0000791; C:euchromatin; IEA:Ensembl.
DR GO; GO:0000178; C:exosome (RNase complex); ISS:UniProtKB.
DR GO; GO:0000176; C:nuclear exosome (RNase complex); IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0031090; C:organelle membrane; IEA:UniProt.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0071034; P:CUT catabolic process; IBA:GO_Central.
DR GO; GO:0045006; P:DNA deamination; IEA:Ensembl.
DR GO; GO:0043928; P:exonucleolytic catabolism of deadenylated mRNA; IBA:GO_Central.
DR GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0045190; P:isotype switching; IEA:Ensembl.
DR GO; GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IBA:GO_Central.
DR GO; GO:0071038; P:nuclear polyadenylation-dependent tRNA catabolic process; IBA:GO_Central.
DR GO; GO:0034427; P:nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'; IBA:GO_Central.
DR GO; GO:0071051; P:polyadenylation-dependent snoRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0045830; P:positive regulation of isotype switching; IEA:Ensembl.
DR GO; GO:0034475; P:U4 snRNA 3'-end processing; IBA:GO_Central.
DR CDD; cd05790; S1_Rrp40; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1370.10; -; 1.
DR InterPro; IPR026699; Exosome_RNA_bind1/RRP40/RRP4.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR037319; Rrp40_S1.
DR PANTHER; PTHR21321; PTHR21321; 1.
DR Pfam; PF15985; KH_6; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; Exosome; Isopeptide bond; Nucleus;
KW Reference proteome; RNA-binding; rRNA processing; Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9NQT5"
FT CHAIN 2..275
FT /note="Exosome complex component RRP40"
FT /id="PRO_0000287529"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9NQT5"
FT CROSSLNK 151
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NQT5"
SQ SEQUENCE 275 AA; 29495 MW; 1552C87EA54C6FA3 CRC64;
MAEAAGVPAE SLAGCRARAA RTVLDQVVLP GEELLLPDQE DGDGPGGAGE RPLRLNAAAR
SRGRVVCGPG LRRCGDRLLV TKCGRLRHKE PGSGSGGGVY WVDSQQKRYV PVKGDHVIGI
VTAKSGDIFK VDVGGSEPAS LSYLAFEGAT KRNRPNVQVG DLIYGQFVVA NKDMEPEMVC
IDSCGRANGM GVIGQDGLLF KVTLGLIRKL LAPDCEILQE VGKLYPLEIV FGMNGRIWVK
AKTIQQTLIL ANILEACEHM TADQRKQIFS RLAES
