EXOS5_ARATH
ID EXOS5_ARATH Reviewed; 239 AA.
AC Q9LX74;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Exosome complex exonuclease RRP46 homolog {ECO:0000305};
DE EC=3.1.13.- {ECO:0000305};
DE AltName: Full=Exosome component 5 {ECO:0000305};
DE AltName: Full=Ribosomal RNA-processing protein 46 {ECO:0000305};
GN Name=RRP46 {ECO:0000305};
GN OrderedLocusNames=At3g46210 {ECO:0000312|Araport:AT3G46210};
GN ORFNames=F12M12.180 {ECO:0000312|EMBL:CAB90948.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable component of the exosome 3'->5' exoribonuclease
CC complex, a complex that degrades inherently unstable mRNAs containing
CC AU-rich elements (AREs) within their 3'-untranslated regions.
CC {ECO:0000250|UniProtKB:Q84T68}.
CC -!- SUBUNIT: Probable component of the RNA exosome complex.
CC {ECO:0000250|UniProtKB:Q84T68}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q84T68}.
CC -!- SIMILARITY: Belongs to the RNase PH family. {ECO:0000305}.
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DR EMBL; AL355775; CAB90948.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78129.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78130.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78131.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78132.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78133.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78134.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63606.1; -; Genomic_DNA.
DR EMBL; BT003125; AAO24557.1; -; mRNA.
DR EMBL; AK317261; BAH19939.1; -; mRNA.
DR EMBL; AK317537; BAH20201.1; -; mRNA.
DR EMBL; AK228171; BAF00127.1; -; mRNA.
DR PIR; T49262; T49262.
DR RefSeq; NP_001030817.1; NM_001035740.3.
DR RefSeq; NP_001030818.1; NM_001035741.2.
DR RefSeq; NP_001030819.1; NM_001035742.3.
DR RefSeq; NP_001078250.1; NM_001084781.2.
DR RefSeq; NP_001190019.1; NM_001203090.1.
DR RefSeq; NP_001325681.1; NM_001339242.1.
DR RefSeq; NP_190207.1; NM_114490.3.
DR AlphaFoldDB; Q9LX74; -.
DR SMR; Q9LX74; -.
DR IntAct; Q9LX74; 2.
DR STRING; 3702.AT3G46210.6; -.
DR PaxDb; Q9LX74; -.
DR PRIDE; Q9LX74; -.
DR ProteomicsDB; 222335; -.
DR EnsemblPlants; AT3G46210.1; AT3G46210.1; AT3G46210.
DR EnsemblPlants; AT3G46210.2; AT3G46210.2; AT3G46210.
DR EnsemblPlants; AT3G46210.3; AT3G46210.3; AT3G46210.
DR EnsemblPlants; AT3G46210.4; AT3G46210.4; AT3G46210.
DR EnsemblPlants; AT3G46210.5; AT3G46210.5; AT3G46210.
DR EnsemblPlants; AT3G46210.6; AT3G46210.6; AT3G46210.
DR EnsemblPlants; AT3G46210.7; AT3G46210.7; AT3G46210.
DR GeneID; 823766; -.
DR Gramene; AT3G46210.1; AT3G46210.1; AT3G46210.
DR Gramene; AT3G46210.2; AT3G46210.2; AT3G46210.
DR Gramene; AT3G46210.3; AT3G46210.3; AT3G46210.
DR Gramene; AT3G46210.4; AT3G46210.4; AT3G46210.
DR Gramene; AT3G46210.5; AT3G46210.5; AT3G46210.
DR Gramene; AT3G46210.6; AT3G46210.6; AT3G46210.
DR Gramene; AT3G46210.7; AT3G46210.7; AT3G46210.
DR KEGG; ath:AT3G46210; -.
DR Araport; AT3G46210; -.
DR TAIR; locus:2075351; AT3G46210.
DR eggNOG; KOG1069; Eukaryota.
DR HOGENOM; CLU_063514_2_3_1; -.
DR InParanoid; Q9LX74; -.
DR OMA; CIINEQG; -.
DR OrthoDB; 1129417at2759; -.
DR PhylomeDB; Q9LX74; -.
DR PRO; PR:Q9LX74; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LX74; baseline and differential.
DR GO; GO:0000177; C:cytoplasmic exosome (RNase complex); IBA:GO_Central.
DR GO; GO:0000176; C:nuclear exosome (RNase complex); IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0004536; F:deoxyribonuclease activity; IEA:EnsemblPlants.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:EnsemblPlants.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblPlants.
DR GO; GO:0004540; F:ribonuclease activity; IEA:EnsemblPlants.
DR GO; GO:0003727; F:single-stranded RNA binding; IEA:EnsemblPlants.
DR GO; GO:0071028; P:nuclear mRNA surveillance; IBA:GO_Central.
DR GO; GO:0034427; P:nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'; IBA:GO_Central.
DR GO; GO:0071051; P:polyadenylation-dependent snoRNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0016075; P:rRNA catabolic process; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0034475; P:U4 snRNA 3'-end processing; IBA:GO_Central.
DR Gene3D; 3.30.230.70; -; 1.
DR InterPro; IPR001247; ExoRNase_PH_dom1.
DR InterPro; IPR036345; ExoRNase_PH_dom2_sf.
DR InterPro; IPR027408; PNPase/RNase_PH_dom_sf.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR Pfam; PF01138; RNase_PH; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55666; SSF55666; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Exonuclease; Exosome; Hydrolase; Nuclease; Nucleus;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA processing.
FT CHAIN 1..239
FT /note="Exosome complex exonuclease RRP46 homolog"
FT /id="PRO_0000435320"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9SP08"
SQ SEQUENCE 239 AA; 25810 MW; 8E22AE4B0899FECE CRC64;
MEIDREDGRT PNQLRPLACS RNILHRPHGS ASWSQGDTKV LAAVYGPKAG TKKNENAEKA
CFEVIWKPKS GQIGKVEKEY EMILKRTIQS ICVLTVNPNT TTSVIIQVVH DDGSLLPCAI
NAACAALVDA GIPMKHLAVA ICCCLAENGY LVLDPNKLEE KKMTAFAYLV FPNTTLSVLP
EGSSVAEGEP VEHGIITSIT HGVMSVDDYF LCVENGRAAT ASLSAFFRKN FQQSSSKAG
