EXOT_RHIME
ID EXOT_RHIME Reviewed; 494 AA.
AC P33699;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Succinoglycan biosynthesis transport protein ExoT;
GN Name=exoT; OrderedLocusNames=RB1074; ORFNames=SMb20950;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymB (megaplasmid 2).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT by Rhizobium meliloti.";
RL J. Bacteriol. 175:7033-7044(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=8226646; DOI=10.1128/jb.175.21.7045-7055.1993;
RA Glucksmann M.A., Reuber T.L., Walker G.C.;
RT "Genes needed for the modification, polymerization, export, and processing
RT of succinoglycan by Rhizobium meliloti: a model for succinoglycan
RT biosynthesis.";
RL J. Bacteriol. 175:7045-7055(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RCR2011 / SU47;
RX PubMed=8118055; DOI=10.1094/mpmi-6-735;
RA Becker A., Kleickmann A., Kuester H., Keller M., Arnold W., Puehler A.;
RT "Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT, and exoI
RT involved in exopolysaccharide biosynthesis and nodule invasion: exoU and
RT exoW probably encode glucosyltransferases.";
RL Mol. Plant Microbe Interact. 6:735-744(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481431; DOI=10.1073/pnas.161294698;
RA Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT endosymbiont Sinorhizobium meliloti.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the polysaccharide synthase family.
CC {ECO:0000305}.
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DR EMBL; L20758; AAA16050.1; -; Unassigned_DNA.
DR EMBL; Z22646; CAA80362.1; -; Genomic_DNA.
DR EMBL; AL591985; CAC49474.1; -; Genomic_DNA.
DR PIR; B95976; B95976.
DR PIR; C49349; C49349.
DR RefSeq; NP_437614.1; NC_003078.1.
DR RefSeq; WP_010975911.1; NC_003078.1.
DR AlphaFoldDB; P33699; -.
DR SMR; P33699; -.
DR STRING; 266834.SM_b20950; -.
DR TCDB; 2.A.66.2.2; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR EnsemblBacteria; CAC49474; CAC49474; SM_b20950.
DR GeneID; 61601031; -.
DR KEGG; sme:SM_b20950; -.
DR PATRIC; fig|266834.11.peg.6003; -.
DR eggNOG; COG2244; Bacteria.
DR HOGENOM; CLU_026911_3_1_5; -.
DR OMA; DLAFYWD; -.
DR BioCyc; MetaCyc:SM_B20950-MON; -.
DR UniPathway; UPA00631; -.
DR Proteomes; UP000001976; Plasmid pSymB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR002797; Polysacc_synth.
DR Pfam; PF01943; Polysacc_synt; 1.
PE 3: Inferred from homology;
KW Cell membrane; Exopolysaccharide synthesis; Membrane; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..494
FT /note="Succinoglycan biosynthesis transport protein ExoT"
FT /id="PRO_0000166447"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 494 AA; 53316 MW; 008521D05FDB6013 CRC64;
MTPTVNAKTV TRNVGWSVLS KTGTFGLKFV TVPILARILS PEEFGAVAVA LTVVQFLAMI
GGAGLTSALV IQQHEEMETV HSVFWANLAI ALMMALGLFV FAEPLATLLG APEAAYLLRI
MSLLIPLQLG GDVAYSLLVR RMNFRKDAVW SMISESLGAV IAVLLALLGF GIWSLLAQLF
VSALVRLSGL YAVSRYAPRF VFSLQRVLAL SRFSFGMMGS EIANFITFQS PMVVISRYLG
LSDAGAYSAA NRFASIPNQV VLSAVMGVLF PTFGQMMHDR ERRSQALMLS TQVTTVLLAP
MMFGLWALAE PAMLVLFGSQ WAYAWPVLGL LALSKGILTP CSTFIPYLKG VGQGAVLFWW
ALIRAVATTG AVAYGAIDGS LVEAMIWLCI VNAVTLVGYS WVVFRADSTP FLKGLFISSR
PMIAALLMAL VVRFLLEHFG AHVPNAVLQL IAGTAIGSVI YTVLILLTER SLLRRLLDMA
RARKPRAAPA GAAE
