ID   EXOU_RHIME              Reviewed;         342 AA.
AC   P33700;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Succinoglycan biosynthesis protein ExoU;
DE            EC=2.4.-.-;
GN   Name=exoU; OrderedLocusNames=RB1071; ORFNames=SMb20948;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA   Glucksmann M.A., Reuber T.L., Walker G.C.;
RT   "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT   by Rhizobium meliloti.";
RL   J. Bacteriol. 175:7033-7044(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RCR2011 / SU47;
RX   PubMed=8118055; DOI=10.1094/mpmi-6-735;
RA   Becker A., Kleickmann A., Kuester H., Keller M., Arnold W., Puehler A.;
RT   "Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT, and exoI
RT   involved in exopolysaccharide biosynthesis and nodule invasion: exoU and
RT   exoW probably encode glucosyltransferases.";
RL   Mol. Plant Microbe Interact. 6:735-744(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Glycosyltransferase required for the synthesis of
CC       succinoglycan (EPS I). Needed for the addition of the sixth sugar
CC       (glucose), catalyzes the formation of a beta-1,6 linkage between the
CC       fifth and sixth sugar.
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; L20758; AAA16053.1; -; Unassigned_DNA.
DR   EMBL; Z22646; CAA80359.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49471.1; -; Genomic_DNA.
DR   PIR; D49348; D49348.
DR   PIR; G95975; G95975.
DR   RefSeq; NP_437611.1; NC_003078.1.
DR   RefSeq; WP_010975909.1; NC_003078.1.
DR   AlphaFoldDB; P33700; -.
DR   SMR; P33700; -.
DR   STRING; 266834.SM_b20948; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; CAC49471; CAC49471; SM_b20948.
DR   GeneID; 61601028; -.
DR   KEGG; sme:SM_b20948; -.
DR   PATRIC; fig|266834.11.peg.6000; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_025996_0_1_5; -.
DR   OMA; RYKIIHS; -.
DR   BioCyc; MetaCyc:SM_B20948-MON; -.
DR   UniPathway; UPA00631; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Exopolysaccharide synthesis; Glycosyltransferase; Plasmid;
KW   Reference proteome; Transferase.
FT   CHAIN           1..342
FT                   /note="Succinoglycan biosynthesis protein ExoU"
FT                   /id="PRO_0000059184"
SQ   SEQUENCE   342 AA;  37018 MW;  EAF55E0EBDA023BC CRC64;
     MTAAAPTDVC IIISAKNAAD TIARAVASAL AEPEAAEVVV IDDGSTDDSA SVARAADDGT
     GRLNVVRFEE NRGPAAARNH AIAISHSPLI GVLDADDFFF PGRLGQLLSQ DGWDFIADNI
     AFIDAAQAAT AHGRIDRFAP TPRLIDLVGF VEGNISRRGV RRGEIGFLKP LMRRAFLDQH
     GLRYNETLRL GEDYDLYARA LANGARYKII HSCGYAAVVR GNSLSGSHRT IDLKRLYEAD
     RAILAGSRLS SDAEAAVRRH ERHIRDRYEL RHFLDLKNQQ GFGRAFGYAL THPAALPAII
     GGILADKTER FRPSGSPAPV ALGGKGDVRY LLETLAVDQP QK