ID   EXOW_RHIME              Reviewed;         319 AA.
AC   P33702; Q52927;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Succinoglycan biosynthesis protein ExoW;
DE            EC=2.4.-.-;
GN   Name=exoW; OrderedLocusNames=RB1073; ORFNames=SMb21690;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=8226645; DOI=10.1128/jb.175.21.7033-7044.1993;
RA   Glucksmann M.A., Reuber T.L., Walker G.C.;
RT   "Family of glycosyl transferases needed for the synthesis of succinoglycan
RT   by Rhizobium meliloti.";
RL   J. Bacteriol. 175:7033-7044(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RCR2011 / SU47;
RX   PubMed=8118055; DOI=10.1094/mpmi-6-735;
RA   Becker A., Kleickmann A., Kuester H., Keller M., Arnold W., Puehler A.;
RT   "Analysis of the Rhizobium meliloti genes exoU, exoV, exoW, exoT, and exoI
RT   involved in exopolysaccharide biosynthesis and nodule invasion: exoU and
RT   exoW probably encode glucosyltransferases.";
RL   Mol. Plant Microbe Interact. 6:735-744(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Glycosyltransferase required for the synthesis of
CC       succinoglycan (EPS I). Needed for the addition of the seventh sugar
CC       (glucose), catalyzes the formation of a beta-1,3 linkage between the
CC       seventh and eighth sugar.
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; L20758; AAA16051.1; -; Unassigned_DNA.
DR   EMBL; Z22646; CAA80361.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49473.1; -; Genomic_DNA.
DR   PIR; A95976; A95976.
DR   PIR; E49348; E49348.
DR   PIR; S40175; S40175.
DR   RefSeq; NP_437613.1; NC_003078.1.
DR   RefSeq; WP_004434930.1; NC_003078.1.
DR   AlphaFoldDB; P33702; -.
DR   SMR; P33702; -.
DR   STRING; 266834.SM_b21690; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; CAC49473; CAC49473; SM_b21690.
DR   GeneID; 25013735; -.
DR   GeneID; 61601030; -.
DR   KEGG; sme:SM_b21690; -.
DR   PATRIC; fig|266834.11.peg.6002; -.
DR   eggNOG; COG0463; Bacteria.
DR   HOGENOM; CLU_072543_0_0_5; -.
DR   OMA; GECYWAS; -.
DR   BioCyc; MetaCyc:SM_B21690-MON; -.
DR   UniPathway; UPA00631; -.
DR   PRO; PR:P33702; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Exopolysaccharide synthesis; Glycosyltransferase; Membrane;
KW   Plasmid; Reference proteome; Transferase.
FT   CHAIN           1..319
FT                   /note="Succinoglycan biosynthesis protein ExoW"
FT                   /id="PRO_0000059185"
FT   CONFLICT        260..319
FT                   /note="KAMEAIRSYKHTARRQALWSQARRIKRRKLPQFDLLARWLWRDPRLIGSAAE
FT                   LAVGKLSR -> NGWRRSDPTSIPRGGRRYGARHAG (in Ref. 1; AAA16051)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   319 AA;  36216 MW;  64BA75EF1C9A7219 CRC64;
     MAKLTVVIPY YQKEPGILRR ALASVFAQTL EDFHVLVIDD ESPYPIADEL AGLAQEERER
     ITVIRQPNGG PGGARNTGLD NVPADSDFVA FLDSDDVWTP DHLLNAYQSM TRFDADCYWA
     SITGGDAFYY HFGVADLEKS ETVTRLSESP LVVELPELQD VMLKNWSFLH MSCMVIGRKL
     FEKVRFEATL KLAAEDVLFF CDCVLASKRV VLCDAAGAVR GEGLNIFHSI DNDSPQFLKQ
     QFNTWVALDT LEGRYRNRPK AMEAIRSYKH TARRQALWSQ ARRIKRRKLP QFDLLARWLW
     RDPRLIGSAA ELAVGKLSR