EXOX_ECOLI
ID EXOX_ECOLI Reviewed; 220 AA.
AC P0AEK0; P76281; Q2MB09;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Exodeoxyribonuclease 10;
DE EC=3.1.11.-;
DE AltName: Full=Exodeoxyribonuclease X;
DE Short=Exo X;
DE Short=Exonuclease X;
GN Name=exoX; Synonyms=yobC; OrderedLocusNames=b1844, JW1833;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RX PubMed=10514496; DOI=10.1074/jbc.274.42.30094;
RA Viswanathan M., Lovett S.T.;
RT "Exonuclease X of Escherichia coli. A novel 3'-5' DNase and DnaQ
RT superfamily member involved in DNA repair.";
RL J. Biol. Chem. 274:30094-30100(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Capable of degrading both single-strand and double-strand DNA
CC with 3' to 5' polarity. Has higher affinity for ssDNA ends than for
CC dsDNA.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
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DR EMBL; AF194116; AAF04847.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74914.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76547.1; -; Genomic_DNA.
DR PIR; D64946; D64946.
DR RefSeq; NP_416358.1; NC_000913.3.
DR RefSeq; WP_000944256.1; NZ_STEB01000009.1.
DR PDB; 4FZX; X-ray; 2.30 A; C/D=1-167.
DR PDB; 4FZY; X-ray; 2.50 A; A/B=1-167.
DR PDB; 4FZZ; X-ray; 2.80 A; A/B=1-167.
DR PDBsum; 4FZX; -.
DR PDBsum; 4FZY; -.
DR PDBsum; 4FZZ; -.
DR AlphaFoldDB; P0AEK0; -.
DR SMR; P0AEK0; -.
DR BioGRID; 4260363; 106.
DR STRING; 511145.b1844; -.
DR jPOST; P0AEK0; -.
DR PaxDb; P0AEK0; -.
DR PRIDE; P0AEK0; -.
DR EnsemblBacteria; AAC74914; AAC74914; b1844.
DR EnsemblBacteria; BAE76547; BAE76547; BAE76547.
DR GeneID; 66674266; -.
DR GeneID; 946361; -.
DR KEGG; ecj:JW1833; -.
DR KEGG; eco:b1844; -.
DR PATRIC; fig|511145.12.peg.1922; -.
DR EchoBASE; EB3785; -.
DR eggNOG; COG0847; Bacteria.
DR HOGENOM; CLU_047806_8_2_6; -.
DR InParanoid; P0AEK0; -.
DR OMA; SPYYVAH; -.
DR PhylomeDB; P0AEK0; -.
DR BioCyc; EcoCyc:G7016-MON; -.
DR PRO; PR:P0AEK0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IDA:EcoCyc.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0045004; P:DNA replication proofreading; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IDA:EcoCyc.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA repair; Exonuclease; Hydrolase; Magnesium;
KW Nuclease; Reference proteome.
FT CHAIN 1..220
FT /note="Exodeoxyribonuclease 10"
FT /id="PRO_0000087136"
FT STRAND 2..11
FT /evidence="ECO:0007829|PDB:4FZX"
FT STRAND 16..25
FT /evidence="ECO:0007829|PDB:4FZX"
FT STRAND 28..36
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 45..51
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 55..58
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 64..67
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:4FZX"
FT STRAND 75..81
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 82..88
FT /evidence="ECO:0007829|PDB:4FZX"
FT STRAND 93..98
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 99..106
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 114..120
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 136..154
FT /evidence="ECO:0007829|PDB:4FZX"
FT HELIX 158..165
FT /evidence="ECO:0007829|PDB:4FZX"
SQ SEQUENCE 220 AA; 25133 MW; A1863A22C8C57A6F CRC64;
MLRIIDTETC GLQGGIVEIA SVDVIDGKIV NPMSHLVRPD RPISPQAMAI HRITEAMVAD
KPWIEDVIPH YYGSEWYVAH NASFDRRVLP EMPGEWICTM KLARRLWPGI KYSNMALYKT
RKLNVQTPPG LHHHRALYDC YITAALLIDI MNTSGWTAEQ MADITGRPSL MTTFTFGKYR
GKAVSDVAER DPGYLRWLFN NLDSMSPELR LTLKHYLENT
