ID   EXO_ARATH               Reviewed;         314 AA.
AC   Q9ZPE7; Q8LB34;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=Protein EXORDIUM;
DE   Flags: Precursor;
GN   Name=EXO; OrderedLocusNames=At4g08950; ORFNames=T3H13.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=12943541; DOI=10.1046/j.1365-313x.2003.01608.x;
RA   Farrar K., Evans I.M., Topping J.F., Souter M.A., Nielsen J.E., Lindsey K.;
RT   "EXORDIUM--a gene expressed in proliferating cells and with a role in
RT   meristem function, identified by promoter trapping in Arabidopsis.";
RL   Plant J. 33:61-73(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   FUNCTION, AND INDUCTION BY EPIBRASSINOLIDE.
RX   PubMed=15063727; DOI=10.1016/s0014-5793(04)00255-8;
RA   Coll-Garcia D., Mazuch J., Altmann T., Mussig C.;
RT   "EXORDIUM regulates brassinosteroid-responsive genes.";
RL   FEBS Lett. 563:82-86(2004).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, GENE FAMILY, NOMENCLATURE, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=19216774; DOI=10.1186/1471-2229-9-20;
RA   Schroder F., Lisso J., Lange P., Mussig C.;
RT   "The extracellular EXO protein mediates cell expansion in Arabidopsis
RT   leaves.";
RL   BMC Plant Biol. 9:20-20(2009).
CC   -!- FUNCTION: Required for cell expansion in leaves. May mediate
CC       brassinosteroid (BR)-induced leaf growth. May play a role in the
CC       control of BR responses in roots. May be involved in signaling
CC       processes that coordinate BR responses with environmental or
CC       developmental signals. {ECO:0000269|PubMed:15063727,
CC       ECO:0000269|PubMed:19216774}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19216774}. Secreted,
CC       extracellular space {ECO:0000269|PubMed:19216774}. Secreted,
CC       extracellular space, apoplast {ECO:0000269|PubMed:19216774}.
CC   -!- TISSUE SPECIFICITY: Expressed in root tips, vascular tissue of roots,
CC       shoot apex, rosette leaves and embryos. {ECO:0000269|PubMed:12943541}.
CC   -!- INDUCTION: By the synthetic auxin naphthaleneacetic acid (NAA) and 24-
CC       epibrassinolide. Down-regulated by kinetin.
CC       {ECO:0000269|PubMed:12943541, ECO:0000269|PubMed:15063727}.
CC   -!- DISRUPTION PHENOTYPE: Reduced leaf size, root length and biomass
CC       production due to diminished expansion of epidermis and parenchyma
CC       cells. {ECO:0000269|PubMed:19216774}.
CC   -!- MISCELLANEOUS: Over-expression of EXO promotes growth in both shoots
CC       and roots. {ECO:0000305|PubMed:15063727}.
CC   -!- SIMILARITY: Belongs to the EXORDIUM family. {ECO:0000305}.
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DR   EMBL; AF496655; AAM18526.1; -; mRNA.
DR   EMBL; AF128396; AAD17365.1; -; Genomic_DNA.
DR   EMBL; AL161513; CAB78019.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE82700.1; -; Genomic_DNA.
DR   EMBL; AY054486; AAK96677.1; -; mRNA.
DR   EMBL; AY093295; AAM13294.1; -; mRNA.
DR   EMBL; AY087446; AAM64992.1; -; mRNA.
DR   PIR; C85090; C85090.
DR   RefSeq; NP_192634.1; NM_116964.3.
DR   AlphaFoldDB; Q9ZPE7; -.
DR   BioGRID; 11772; 1.
DR   STRING; 3702.AT4G08950.1; -.
DR   PaxDb; Q9ZPE7; -.
DR   PRIDE; Q9ZPE7; -.
DR   ProteomicsDB; 222303; -.
DR   EnsemblPlants; AT4G08950.1; AT4G08950.1; AT4G08950.
DR   GeneID; 826473; -.
DR   Gramene; AT4G08950.1; AT4G08950.1; AT4G08950.
DR   KEGG; ath:AT4G08950; -.
DR   Araport; AT4G08950; -.
DR   TAIR; locus:2138753; AT4G08950.
DR   eggNOG; KOG0017; Eukaryota.
DR   HOGENOM; CLU_053777_1_0_1; -.
DR   InParanoid; Q9ZPE7; -.
DR   OMA; THIEEIM; -.
DR   OrthoDB; 888488at2759; -.
DR   PhylomeDB; Q9ZPE7; -.
DR   PRO; PR:Q9ZPE7; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9ZPE7; baseline and differential.
DR   Genevisible; Q9ZPE7; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR   GO; GO:0009505; C:plant-type cell wall; IDA:TAIR.
DR   GO; GO:0009741; P:response to brassinosteroid; IMP:TAIR.
DR   InterPro; IPR006766; EXORDIUM-like.
DR   PANTHER; PTHR31279; PTHR31279; 1.
DR   Pfam; PF04674; Phi_1; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..314
FT                   /note="Protein EXORDIUM"
FT                   /id="PRO_0000430280"
FT   CONFLICT        3
FT                   /note="L -> S (in Ref. 5; AAM64992)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   314 AA;  33599 MW;  D5E4AC91F159B83A CRC64;
     MYLLVFKLFL FLSLLQISVS ARNLASQEPN QFQLLKYHKG ALLSGKISVN LIWYGKFKPS
     QRAIISDFIT SLTHTSPTSK TLHQPSVATW WKTTEKYYKL ATPSKNSSPL SLTLGKQIID
     ESCSLGKSLT DKKIQTLASK GDQRNAINVV LTSADVTVTG FGMSRCGTHG HARGLGKRGS
     KFAYIWVGNS ETQCPGQCAW PFHAPVYGPQ SPPLVAPNND VGLDGMVINL ASLLAGTATN
     PFGNGYYQGP QNAPLEAASA CPGVYGKGAY PGYAGDLLVD TTTGGSFNAY GANGRKFLLP
     ALYDPTTSAC STMV