EXPA1_ARATH
ID EXPA1_ARATH Reviewed; 250 AA.
AC Q9C554; Q38863;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Expansin-A1;
DE Short=AtEXPA1;
DE AltName: Full=Alpha-expansin-1;
DE Short=At-EXP1;
DE Short=AtEx1;
DE AltName: Full=Ath-ExpAlpha-1.2;
DE Flags: Precursor;
GN Name=EXPA1; Synonyms=EXP1; OrderedLocusNames=At1g69530; ORFNames=F10D13_18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 14-250.
RX PubMed=7568110; DOI=10.1073/pnas.92.20.9245;
RA Shcherban T.Y., Shi J., Durachko D.M., Guiltinan M.J., McQueen-Mason S.J.,
RA Shieh M., Cosgrove D.J.;
RT "Molecular cloning and sequence analysis of expansins - a highly conserved,
RT multigene family of proteins that mediate cell wall extension in plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:9245-9249(1995).
RN [5]
RP TISSUE SPECIFICITY.
RA Durachko D.M., Cosgrove D.J.;
RT "Expression patterns for selective expansin genes in Arabidopsis.";
RL (In) Proceedings of Plant Biology '99: The annual meeting of the American
RL Society of Plant Physiologists, abstract#56, Baltimore (1999).
RN [6]
RP NOMENCLATURE.
RX PubMed=15604683; DOI=10.1007/s11103-004-0158-6;
RA Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J.,
RA Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C.,
RA Voesenek L.A.C.;
RT "Nomenclature for members of the expansin superfamily of genes and
RT proteins.";
RL Plant Mol. Biol. 55:311-314(2004).
CC -!- FUNCTION: Causes loosening and extension of plant cell walls by
CC disrupting non-covalent bonding between cellulose microfibrils and
CC matrix glucans. No enzymatic activity has been found (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Peripheral
CC membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9C554-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in stomatal guard cells and very young
CC vascular bundles throughout the plant. {ECO:0000269|Ref.5}.
CC -!- SIMILARITY: Belongs to the expansin family. Expansin A subfamily.
CC {ECO:0000305}.
CC -!- WEB RESOURCE: Name=EXPANSIN homepage;
CC URL="http://homes.bio.psu.edu/expansins/";
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DR EMBL; AC073178; AAG60095.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34942.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34944.1; -; Genomic_DNA.
DR EMBL; AF360291; AAK26001.1; -; mRNA.
DR EMBL; AY051047; AAK93724.1; -; mRNA.
DR EMBL; U30476; AAB38070.1; -; mRNA.
DR PIR; T50654; T50654.
DR RefSeq; NP_177112.1; NM_105622.2. [Q9C554-1]
DR RefSeq; NP_849868.1; NM_179537.5. [Q9C554-1]
DR AlphaFoldDB; Q9C554; -.
DR SMR; Q9C554; -.
DR BioGRID; 28509; 3.
DR IntAct; Q9C554; 2.
DR STRING; 3702.AT1G69530.3; -.
DR PaxDb; Q9C554; -.
DR PRIDE; Q9C554; -.
DR ProteomicsDB; 222334; -. [Q9C554-1]
DR EnsemblPlants; AT1G69530.1; AT1G69530.1; AT1G69530. [Q9C554-1]
DR EnsemblPlants; AT1G69530.2; AT1G69530.2; AT1G69530. [Q9C554-1]
DR GeneID; 843288; -.
DR Gramene; AT1G69530.1; AT1G69530.1; AT1G69530. [Q9C554-1]
DR Gramene; AT1G69530.2; AT1G69530.2; AT1G69530. [Q9C554-1]
DR KEGG; ath:AT1G69530; -.
DR Araport; AT1G69530; -.
DR eggNOG; ENOG502QPUJ; Eukaryota.
DR InParanoid; Q9C554; -.
DR OMA; FEIRCAS; -.
DR PhylomeDB; Q9C554; -.
DR PRO; PR:Q9C554; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C554; baseline and differential.
DR Genevisible; Q9C554; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IEA:UniProt.
DR GO; GO:0009664; P:plant-type cell wall organization; IEA:InterPro.
DR Gene3D; 2.40.40.10; -; 1.
DR Gene3D; 2.60.40.760; -; 1.
DR InterPro; IPR007118; Expan_Lol_pI.
DR InterPro; IPR002963; Expansin.
DR InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR InterPro; IPR007117; Expansin_CBD.
DR InterPro; IPR036749; Expansin_CBD_sf.
DR InterPro; IPR009009; RlpA-like_DPBB.
DR InterPro; IPR036908; RlpA-like_sf.
DR PANTHER; PTHR31867; PTHR31867; 1.
DR Pfam; PF03330; DPBB_1; 1.
DR Pfam; PF01357; Expansin_C; 1.
DR PRINTS; PR01226; EXPANSIN.
DR PRINTS; PR01225; EXPANSNFAMLY.
DR SMART; SM00837; DPBB_1; 1.
DR SUPFAM; SSF49590; SSF49590; 1.
DR SUPFAM; SSF50685; SSF50685; 1.
DR PROSITE; PS50843; EXPANSIN_CBD; 1.
DR PROSITE; PS50842; EXPANSIN_EG45; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell wall; Cell wall biogenesis/degradation;
KW Disulfide bond; Membrane; Reference proteome; Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..250
FT /note="Expansin-A1"
FT /id="PRO_0000008682"
FT DOMAIN 45..157
FT /note="Expansin-like EG45"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DOMAIN 167..246
FT /note="Expansin-like CBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT DISULFID 48..76
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 79..152
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 84..91
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT CONFLICT 14..18
FT /note="AMTSH -> NSARD (in Ref. 4; AAB38070)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 250 AA; 26518 MW; 1D95EBA24FCFE7E5 CRC64;
MALVTFLFIA TLGAMTSHVN GYAGGGWVNA HATFYGGGDA SGTMGGACGY GNLYSQGYGT
NTAALSTALF NNGLSCGACF EIRCQNDGKW CLPGSIVVTA TNFCPPNNAL PNNAGGWCNP
PQQHFDLSQP VFQRIAQYRA GIVPVAYRRV PCVRRGGIRF TINGHSYFNL VLITNVGGAG
DVHSAMVKGS RTGWQAMSRN WGQNWQSNSY LNGQSLSFKV TTSDGQTIVS NNVANAGWSF
GQTFTGAQLR
