EXPB1_ARATH
ID EXPB1_ARATH Reviewed; 271 AA.
AC Q9SKU2; O23688;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Expansin-B1;
DE Short=At-EXPB1;
DE Short=AtEXPB1;
DE AltName: Full=Ath-ExpBeta-1.5;
DE AltName: Full=Beta-expansin-1;
DE Flags: Precursor;
GN Name=EXPB1; OrderedLocusNames=At2g20750; ORFNames=F5H14.28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 13-271.
RC STRAIN=cv. Columbia;
RX PubMed=7568110; DOI=10.1073/pnas.92.20.9245;
RA Shcherban T.Y., Shi J., Durachko D.M., Guiltinan M.J., McQueen-Mason S.J.,
RA Shieh M., Cosgrove D.J.;
RT "Molecular cloning and sequence analysis of expansins - a highly conserved,
RT multigene family of proteins that mediate cell wall extension in plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:9245-9249(1995).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 13-271.
RC STRAIN=cv. Columbia;
RX PubMed=9177257; DOI=10.1073/pnas.94.12.6559;
RA Cosgrove D.J., Bedinger P.A., Durachko D.M.;
RT "Group I allergens of grass pollen as cell wall-loosening agents.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:6559-6564(1997).
RN [7]
RP NOMENCLATURE.
RX PubMed=15604683; DOI=10.1007/s11103-004-0158-6;
RA Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J.,
RA Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C.,
RA Voesenek L.A.C.;
RT "Nomenclature for members of the expansin superfamily of genes and
RT proteins.";
RL Plant Mol. Biol. 55:311-314(2004).
CC -!- FUNCTION: May cause loosening and extension of plant cell walls by
CC disrupting non-covalent bonding between cellulose microfibrils and
CC matrix glucans. No enzymatic activity has been found (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Peripheral
CC membrane protein.
CC -!- SIMILARITY: Belongs to the expansin family. Expansin B subfamily.
CC {ECO:0000305}.
CC -!- WEB RESOURCE: Name=EXPANSIN homepage;
CC URL="http://homes.bio.psu.edu/expansins/";
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DR EMBL; AC006234; AAD20920.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07066.1; -; Genomic_DNA.
DR EMBL; BT003932; AAO41979.1; -; mRNA.
DR EMBL; BT005024; AAO50557.1; -; mRNA.
DR EMBL; AY084478; AAM61049.1; -; mRNA.
DR EMBL; U95967; AAB61709.1; -; mRNA.
DR PIR; H84592; H84592.
DR PIR; T50657; T50657.
DR RefSeq; NP_179668.1; NM_127640.3.
DR AlphaFoldDB; Q9SKU2; -.
DR SMR; Q9SKU2; -.
DR STRING; 3702.AT2G20750.1; -.
DR PaxDb; Q9SKU2; -.
DR PRIDE; Q9SKU2; -.
DR ProteomicsDB; 222336; -.
DR EnsemblPlants; AT2G20750.1; AT2G20750.1; AT2G20750.
DR GeneID; 816604; -.
DR Gramene; AT2G20750.1; AT2G20750.1; AT2G20750.
DR KEGG; ath:AT2G20750; -.
DR Araport; AT2G20750; -.
DR TAIR; locus:2051338; AT2G20750.
DR eggNOG; ENOG502QPVQ; Eukaryota.
DR HOGENOM; CLU_027462_1_2_1; -.
DR InParanoid; Q9SKU2; -.
DR OMA; GQNIAFH; -.
DR OrthoDB; 1148685at2759; -.
DR PhylomeDB; Q9SKU2; -.
DR PRO; PR:Q9SKU2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKU2; baseline and differential.
DR Genevisible; Q9SKU2; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0019953; P:sexual reproduction; IEA:InterPro.
DR GO; GO:0006949; P:syncytium formation; IBA:GO_Central.
DR Gene3D; 2.40.40.10; -; 1.
DR Gene3D; 2.60.40.760; -; 1.
DR InterPro; IPR007118; Expan_Lol_pI.
DR InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR InterPro; IPR007117; Expansin_CBD.
DR InterPro; IPR036749; Expansin_CBD_sf.
DR InterPro; IPR005795; LolPI.
DR InterPro; IPR009009; RlpA-like_DPBB.
DR InterPro; IPR036908; RlpA-like_sf.
DR Pfam; PF03330; DPBB_1; 1.
DR Pfam; PF01357; Expansin_C; 1.
DR PRINTS; PR01225; EXPANSNFAMLY.
DR PRINTS; PR00829; LOLP1ALLERGN.
DR SMART; SM00837; DPBB_1; 1.
DR SUPFAM; SSF49590; SSF49590; 1.
DR SUPFAM; SSF50685; SSF50685; 1.
DR PROSITE; PS50843; EXPANSIN_CBD; 1.
DR PROSITE; PS50842; EXPANSIN_EG45; 1.
PE 2: Evidence at transcript level;
KW Cell wall; Cell wall biogenesis/degradation; Disulfide bond; Glycoprotein;
KW Membrane; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..271
FT /note="Expansin-B1"
FT /id="PRO_0000008707"
FT DOMAIN 58..169
FT /note="Expansin-like EG45"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DOMAIN 182..263
FT /note="Expansin-like CBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT CARBOHYD 242
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 61..90
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 93..164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 98..104
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
SQ SEQUENCE 271 AA; 29108 MW; 2502FFC4D16687E3 CRC64;
MQLFPVILPT LCVFLHLLIS GSGSTPPLTH SNQQVAATRW LPATATWYGS AEGDGSSGGA
CGYGSLVDVK PFKARVGAVS PILFKGGEGC GACYKVRCLD KTICSKRAVT IIATDQSPSG
PSAKAKHTHF DLSGAAFGHM AIPGHNGVIR NRGLLNILYR RTACKYRGKN IAFHVNAGST
DYWLSLLIEY EDGEGDIGSM HIRQAGSKEW ISMKHIWGAN WCIVEGPLKG PFSVKLTTLS
NNKTLSATDV IPSNWVPKAT YTSRLNFSPV L
