EXPB1_PASNO
ID EXPB1_PASNO Reviewed; 265 AA.
AC B8PYF3;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=Expansin-B1 {ECO:0000305};
DE AltName: Full=BaGP allergen Pas n 1 {ECO:0000303|PubMed:18817975};
DE AltName: Full=Beta-expansin-1 {ECO:0000305};
DE AltName: Full=Major group 1 allergen Pas n 1 {ECO:0000303|PubMed:18817975, ECO:0000303|PubMed:20962534, ECO:0000303|PubMed:21231976};
DE AltName: Full=Major pollen allergen Pas n 1 {ECO:0000303|PubMed:18817975, ECO:0000303|PubMed:21231976, ECO:0000303|PubMed:22832594, ECO:0000303|PubMed:25612605};
DE AltName: Full=Pas n 1 allergen {ECO:0000312|EMBL:ACA23876.1};
DE AltName: Allergen=Pas n 1.0101 {ECO:0000303|PubMed:18817975};
DE Flags: Precursor;
OS Paspalum notatum (Bahia grass).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Paspaleae; Paspalinae; Paspalum.
OX NCBI_TaxID=147272 {ECO:0000312|EMBL:ACA23876.1};
RN [1] {ECO:0000312|EMBL:ACA23876.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-32, TISSUE SPECIFICITY,
RP GLYCOSYLATION, ALLERGEN, BIOTECHNOLOGY, AND PHYLOGENETIC ANALYSIS.
RC TISSUE=Pollen {ECO:0000303|PubMed:18817975, ECO:0000312|EMBL:ACA23876.1};
RX PubMed=18817975; DOI=10.1016/j.molimm.2008.08.267;
RA Davies J.M., Mittag D., Dang T.D., Symons K., Voskamp A., Rolland J.M.,
RA O'Hehir R.E.;
RT "Molecular cloning, expression and immunological characterisation of Pas n
RT 1, the major allergen of Bahia grass Paspalum notatum pollen.";
RL Mol. Immunol. 46:286-293(2008).
RN [2]
RP TISSUE SPECIFICITY, ALLERGEN, AND BIOTECHNOLOGY.
RX PubMed=21231976; DOI=10.1111/j.1365-2222.2010.03670.x;
RA Davies J.M., Dang T.D., Voskamp A., Drew A.C., Biondo M., Phung M.,
RA Upham J.W., Rolland J.M., O'Hehir R.E.;
RT "Functional immunoglobulin E cross-reactivity between Pas n 1 of Bahia
RT grass pollen and other group 1 grass pollen allergens.";
RL Clin. Exp. Allergy 41:281-291(2011).
RN [3]
RP TISSUE SPECIFICITY, ALLERGEN, AND BIOTECHNOLOGY.
RX PubMed=20962534; DOI=10.1159/000321821;
RA Drew A.C., Davies J.M., Dang T.D., Rolland J.M., O'Hehir R.E.;
RT "Purification of the major group 1 allergen from Bahia grass pollen, Pas n
RT 1.";
RL Int. Arch. Allergy Immunol. 154:295-298(2011).
RN [4]
RP TISSUE SPECIFICITY, ALLERGEN, BIOTECHNOLOGY, AND REGIONS.
RX PubMed=22832594; DOI=10.1159/000338290;
RA Etto T., de Boer C., Prickett S., Gardner L.M., Voskamp A., Davies J.M.,
RA O'Hehir R.E., Rolland J.M.;
RT "Unique and cross-reactive T cell epitope peptides of the major Bahia grass
RT pollen allergen, Pas n 1.";
RL Int. Arch. Allergy Immunol. 159:355-366(2012).
RN [5]
RP TISSUE SPECIFICITY, ALLERGEN, AND BIOTECHNOLOGY.
RX PubMed=25612605; DOI=10.1159/000369341;
RA Timbrell V.L., Riebelt L., Simmonds C., Solley G., Smith W.B.,
RA Mclean-Tooke A., van Nunen S., Smith P.K., Upham J.W., Langguth D.,
RA Davies J.M.;
RT "An immunodiagnostic assay for quantitation of specific IgE to the major
RT pollen allergen component, Pas n 1, of the subtropical Bahia grass.";
RL Int. Arch. Allergy Immunol. 165:219-228(2014).
CC -!- FUNCTION: May cause loosening of the cell walls.
CC {ECO:0000250|UniProtKB:Q07154}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000250|UniProtKB:Q336T5}.
CC -!- TISSUE SPECIFICITY: Expressed in pollen (at protein level)
CC (PubMed:18817975, PubMed:21231976, PubMed:20962534, PubMed:22832594,
CC PubMed:25612605). {ECO:0000269|PubMed:18817975,
CC ECO:0000269|PubMed:20962534, ECO:0000269|PubMed:21231976,
CC ECO:0000269|PubMed:22832594, ECO:0000269|PubMed:25612605}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:18817975}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to the pollen of grasses (PubMed:18817975,
CC PubMed:21231976, PubMed:20962534, PubMed:22832594, PubMed:25612605).
CC Natural protein binds to IgE in all 21 patients tested strongly
CC allergic to Bahia grass pollen (BaGP). Recombinant protein binds to IgE
CC in 85% of the 55 BaGP-allergic patients tested. Lack of glycosylation
CC does not affect IgE-binding activity (PubMed:18817975). Natural protein
CC binds to IgE in 91% of the 51 BaGP-allergic patients tested
CC (PubMed:20962534). Natural protein reacts with monoclonal antibody
CC against pollen allergen Lol p 1 (PubMed:18817975 PubMed:21231976,
CC PubMed:20962534, PubMed:25612605). Both natural and recombinant protein
CC can up-regulate CD63 expression on the surface of patients basophils
CC (PubMed:18817975 PubMed:21231976). IgE of patients allergic to this
CC protein cross-reacts with pollen allergens Lol p 1 and Cyn d 1
CC (PubMed:21231976, PubMed:22832594). At least one of the three
CC immunodominant T cell epitopes evoke peripheral blood mononuclear cell
CC (PBMC) proliferation in 83% of the 18 BaGP-allergic patients tested. T
CC cell epitopes can also induce secretion of cytokines IL-4, IL-5, IL-10,
CC IFN-gamma and TNF-alpha in BaGP-allergic patients, but do not bind IgE
CC (PubMed:22832594). {ECO:0000269|PubMed:18817975,
CC ECO:0000269|PubMed:20962534, ECO:0000269|PubMed:21231976,
CC ECO:0000269|PubMed:22832594, ECO:0000269|PubMed:25612605}.
CC -!- BIOTECHNOLOGY: This protein has potential use in diagnosis and specific
CC immunotherapy (SIT) of grass pollen allergy for patients living in a
CC subtropical climate (PubMed:18817975, PubMed:21231976, PubMed:20962534,
CC PubMed:25612605). The diagnostic sensitivity and specificity is 92.4%
CC and 93.1%, respectively, for the detection of Bahia grass pollen (BaGP)
CC allergy in patients that have sensitivity level of 0.23 kU/l of IgE for
CC this protein (PubMed:25612605). The immunodominant T cell epitopes may
CC be suitable for safe SIT for individuals with severe allergic symptoms
CC to BaGP (PubMed:22832594). {ECO:0000269|PubMed:25612605,
CC ECO:0000305|PubMed:18817975, ECO:0000305|PubMed:20962534,
CC ECO:0000305|PubMed:21231976, ECO:0000305|PubMed:22832594}.
CC -!- SIMILARITY: Belongs to the expansin family. Expansin B subfamily.
CC {ECO:0000305}.
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DR EMBL; EU327342; ACA23876.1; -; mRNA.
DR SMR; B8PYF3; -.
DR Allergome; 4028; Pas n 1.0101.
DR Allergome; 874; Pas n 1.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IEA:UniProt.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0019953; P:sexual reproduction; IEA:InterPro.
DR Gene3D; 2.40.40.10; -; 1.
DR Gene3D; 2.60.40.760; -; 1.
DR InterPro; IPR007118; Expan_Lol_pI.
DR InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR InterPro; IPR007117; Expansin_CBD.
DR InterPro; IPR036749; Expansin_CBD_sf.
DR InterPro; IPR005795; LolPI.
DR InterPro; IPR009009; RlpA-like_DPBB.
DR InterPro; IPR036908; RlpA-like_sf.
DR Pfam; PF03330; DPBB_1; 1.
DR Pfam; PF01357; Expansin_C; 1.
DR PRINTS; PR01225; EXPANSNFAMLY.
DR PRINTS; PR00829; LOLP1ALLERGN.
DR SMART; SM00837; DPBB_1; 1.
DR SUPFAM; SSF49590; SSF49590; 1.
DR SUPFAM; SSF50685; SSF50685; 1.
DR PROSITE; PS50843; EXPANSIN_CBD; 1.
DR PROSITE; PS50842; EXPANSIN_EG45; 1.
PE 1: Evidence at protein level;
KW Allergen; Cell wall; Cell wall biogenesis/degradation;
KW Direct protein sequencing; Disulfide bond; Glycoprotein; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255, ECO:0000269|PubMed:18817975"
FT CHAIN 24..265
FT /note="Expansin-B1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:18817975"
FT /id="PRO_5002877465"
FT DOMAIN 62..167
FT /note="Expansin-like EG45"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DOMAIN 181..261
FT /note="Expansin-like CBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT REGION 123..142
FT /note="Immunodominant T cell epitope"
FT /evidence="ECO:0000269|PubMed:22832594"
FT REGION 150..169
FT /note="Subdominant T cell epitope; peripheral blood
FT mononuclear cell (PBMC) proliferation response in 28% of
FT the 18 Bahia grass pollen (BaGP)-allergic patients tested"
FT /evidence="ECO:0000269|PubMed:22832594"
FT REGION 177..196
FT /note="Immunodominant T cell epitope"
FT /evidence="ECO:0000269|PubMed:22832594"
FT REGION 213..232
FT /note="Subdominant T cell epitope; peripheral blood
FT mononuclear cell (PBMC) proliferation response in 28% of
FT the 18 Bahia grass pollen (BaGP)-allergic patients tested"
FT /evidence="ECO:0000269|PubMed:22832594"
FT REGION 240..259
FT /note="Immunodominant T cell epitope"
FT /evidence="ECO:0000269|PubMed:22832594"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498,
FT ECO:0000305|PubMed:18817975"
FT DISULFID 65..93
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 96..162
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 101..106
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT CONFLICT 29
FT /note="P -> A (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="P -> K (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 265 AA; 28775 MW; A63220F3B8415155 CRC64;
MGSLAKIVAV AAVLAALVAG GSCGPPKVPP GPNITTNYNG KWLPAKATWY GQPNGAGPDD
NGGACGIKNV NLPPYNGFTA CGNPPIFKDG KGCGSCYEIR CNKPECSGQP VTVFITDMNY
EPIAPYHFDL SGKAFGAMAK PGLNDKLRHY GIFDLEFRRV RCKYQGGQKI VFHVEKGSNP
NYLAMLVKFV ADDGDIVLME LKEKSSDWKP MKLSWGAIWR MDTPKALVPP FSIRLTSESG
KKVIAQDVIP VNWKPDTVYN SNVQF
