ID   EXPB3_ORYSJ             Reviewed;         268 AA.
AC   Q336T5; Q9LD11;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Expansin-B3;
DE   AltName: Full=Beta-expansin-3;
DE   AltName: Full=OsEXPB3;
DE   AltName: Full=OsaEXPb1.10;
DE   Flags: Precursor;
GN   Name=EXPB3; OrderedLocusNames=Os10g0555900, LOC_Os10g40720;
GN   ORFNames=OSJNBa0010C11.1, OSJNBb0014I11.1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9177257; DOI=10.1073/pnas.94.12.6559;
RA   Cosgrove D.J., Bedinger P.A., Durachko D.M.;
RT   "Group I allergens of grass pollen as cell wall-loosening agents.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:6559-6564(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12791992; DOI=10.1126/science.1083523;
RA   Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA   Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA   Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA   Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA   Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA   Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA   Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA   Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA   Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA   Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA   Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA   Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA   Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA   Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA   Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA   Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA   Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT   "In-depth view of structure, activity, and evolution of rice chromosome
RT   10.";
RL   Science 300:1566-1569(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=11598238; DOI=10.1104/pp.010345;
RA   Lee Y., Kende H.;
RT   "Expression of beta-expansins is correlated with internodal elongation in
RT   deepwater rice.";
RL   Plant Physiol. 127:645-654(2001).
RN   [7]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=12428004; DOI=10.1104/pp.008888;
RA   Lee Y., Kende H.;
RT   "Expression of alpha-expansin and expansin-like genes in deepwater rice.";
RL   Plant Physiol. 130:1396-1405(2002).
RN   [8]
RP   NOMENCLATURE.
RX   PubMed=15604683; DOI=10.1007/s11103-004-0158-6;
RA   Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J.,
RA   Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C.,
RA   Voesenek L.A.C.;
RT   "Nomenclature for members of the expansin superfamily of genes and
RT   proteins.";
RL   Plant Mol. Biol. 55:311-314(2004).
RN   [9]
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=16258250;
RA   Lee Y., Choi D.;
RT   "Biochemical properties and localization of the beta-expansin OsEXPB3 in
RT   rice (Oryza sativa L.).";
RL   Mol. Cells 20:119-126(2005).
CC   -!- FUNCTION: May cause loosening and extension of plant cell walls by
CC       disrupting non-covalent bonding between cellulose microfibrils and
CC       matrix glucans. No enzymatic activity has been found. May be required
CC       for rapid internodal elongation in deepwater rice during submergence
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC       {ECO:0000269|PubMed:16258250}. Membrane {ECO:0000269|PubMed:16258250};
CC       Peripheral membrane protein {ECO:0000269|PubMed:16258250}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, coleoptiles and internodes.
CC       {ECO:0000269|PubMed:11598238, ECO:0000269|PubMed:16258250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the growing regions of roots,
CC       coleoptiles, and internodes. {ECO:0000269|PubMed:12428004}.
CC   -!- INDUCTION: By gibberellin (GA3) and wounding.
CC       {ECO:0000269|PubMed:11598238}.
CC   -!- SIMILARITY: Belongs to the expansin family. Expansin B subfamily.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=EXPANSIN homepage;
CC       URL="http://homes.bio.psu.edu/expansins/";
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DR   EMBL; AF261271; AAF72984.1; -; mRNA.
DR   EMBL; AC037426; AAK15453.1; -; Genomic_DNA.
DR   EMBL; AC069300; AAK55465.1; -; Genomic_DNA.
DR   EMBL; DP000086; ABB47975.2; -; Genomic_DNA.
DR   EMBL; AP008216; BAF27188.1; -; Genomic_DNA.
DR   EMBL; AP014966; BAT11995.1; -; Genomic_DNA.
DR   RefSeq; XP_015612911.1; XM_015757425.1.
DR   AlphaFoldDB; Q336T5; -.
DR   SMR; Q336T5; -.
DR   STRING; 4530.OS10T0555900-02; -.
DR   PaxDb; Q336T5; -.
DR   PRIDE; Q336T5; -.
DR   EnsemblPlants; Os10t0555900-02; Os10t0555900-02; Os10g0555900.
DR   GeneID; 4349349; -.
DR   Gramene; Os10t0555900-02; Os10t0555900-02; Os10g0555900.
DR   KEGG; osa:4349349; -.
DR   eggNOG; ENOG502QRTE; Eukaryota.
DR   HOGENOM; CLU_027462_1_0_1; -.
DR   InParanoid; Q336T5; -.
DR   OMA; QIRCIND; -.
DR   OrthoDB; 754048at2759; -.
DR   Proteomes; UP000000763; Chromosome 10.
DR   Proteomes; UP000059680; Chromosome 10.
DR   ExpressionAtlas; Q336T5; baseline and differential.
DR   Genevisible; Q336T5; OS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009653; P:anatomical structure morphogenesis; IEA:UniProt.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0019953; P:sexual reproduction; IEA:InterPro.
DR   Gene3D; 2.40.40.10; -; 1.
DR   Gene3D; 2.60.40.760; -; 1.
DR   InterPro; IPR007118; Expan_Lol_pI.
DR   InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR   InterPro; IPR007117; Expansin_CBD.
DR   InterPro; IPR036749; Expansin_CBD_sf.
DR   InterPro; IPR005795; LolPI.
DR   InterPro; IPR009009; RlpA-like_DPBB.
DR   InterPro; IPR036908; RlpA-like_sf.
DR   Pfam; PF03330; DPBB_1; 1.
DR   Pfam; PF01357; Expansin_C; 1.
DR   PRINTS; PR01225; EXPANSNFAMLY.
DR   PRINTS; PR00829; LOLP1ALLERGN.
DR   SMART; SM00837; DPBB_1; 1.
DR   SUPFAM; SSF49590; SSF49590; 1.
DR   SUPFAM; SSF50685; SSF50685; 1.
DR   PROSITE; PS50843; EXPANSIN_CBD; 1.
DR   PROSITE; PS50842; EXPANSIN_EG45; 1.
PE   2: Evidence at transcript level;
KW   Cell wall; Cell wall biogenesis/degradation; Disulfide bond; Glycoprotein;
KW   Membrane; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..268
FT                   /note="Expansin-B3"
FT                   /id="PRO_0000252014"
FT   DOMAIN          62..168
FT                   /note="Expansin-like EG45"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT   DOMAIN          181..262
FT                   /note="Expansin-like CBD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT   CARBOHYD        30
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        238
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        65..93
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT   DISULFID        96..163
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT   DISULFID        101..107
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
SQ   SEQUENCE   268 AA;  29192 MW;  0EF50BFB816A82BC CRC64;
     MAFSISKKAA VAALFSFLVV TCVAGARPGN FSASDFTADP NWEVARATWY GAPTGAGPDD
     DGGACGFKNT NQYPFSSMTS CGNEPIFKDG KGCGSCYQIR CVNHPACSGN PETVIITDMN
     YYPVSKYHFD LSGTAFGAMA KPGQNDQLRH AGIIDIQFKR VPCNFPGLKV TFHVEEGSNP
     VYFAVLVEYE DGDGDVVQVD LMEANSQSWT PMRESWGSIW RLDSNHRLTA PFSLRITNES
     GKQLVASQVI PANWAPMAVY RSFVQYSS