EXPL3_DICDI
ID EXPL3_DICDI Reviewed; 335 AA.
AC Q7KWS2; Q551W0;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Expansin-like protein 3;
DE Short=Ddexpl3;
DE Flags: Precursor;
GN Name=expl3; ORFNames=DDB_G0276287;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION.
RX PubMed=11891242; DOI=10.1104/pp.010658;
RA Li Y., Darley C.P., Ongaro V., Fleming A., Schipper O., Baldauf S.L.,
RA McQueen-Mason S.J.;
RT "Plant expansins are a complex multigene family with an ancient
RT evolutionary origin.";
RL Plant Physiol. 128:854-864(2002).
RN [4]
RP DEVELOPMENTAL STAGE, AND FUNCTION.
RX PubMed=12832080; DOI=10.1016/s0014-5793(03)00598-2;
RA Darley C.P., Li Y., Schaap P., McQueen-Mason S.J.;
RT "Expression of a family of expansin-like proteins during the development of
RT Dictyostelium discoideum.";
RL FEBS Lett. 546:416-418(2003).
RN [5]
RP IDENTIFICATION.
RX PubMed=15470253; DOI=10.1128/ec.3.5.1241-1248.2004;
RA Maruo T., Sakamoto H., Iranfar N., Fuller D., Morio T., Urushihara H.,
RA Tanaka Y., Maeda M., Loomis W.F.;
RT "Control of cell type proportioning in Dictyostelium discoideum by
RT differentiation-inducing factor as determined by in situ hybridization.";
RL Eukaryot. Cell 3:1241-1248(2004).
RN [6]
RP IDENTIFICATION.
RX PubMed=17481898; DOI=10.1016/j.cub.2007.04.029;
RA Bakthavatsalam D., Brazill D., Gomer R.H., Eichinger L., Rivero F.,
RA Noegel A.A.;
RT "A G protein-coupled receptor with a lipid kinase domain is involved in
RT cell-density sensing.";
RL Curr. Biol. 17:892-897(2007).
RN [7]
RP IDENTIFICATION.
RX PubMed=17878305; DOI=10.1073/pnas.0705996104;
RA Van Driessche N., Alexander H., Min J., Kuspa A., Alexander S.,
RA Shaulsky G.;
RT "Global transcriptional responses to cisplatin in Dictyostelium discoideum
RT identify potential drug targets.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:15406-15411(2007).
CC -!- FUNCTION: May serve to lubricate the movement of the cellulose
CC microfibrils during cell growth and wall extension and/or may serve to
CC maintain the fluid state of the slug cell wall.
CC {ECO:0000269|PubMed:11891242, ECO:0000269|PubMed:12832080}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed at substantial levels throughout
CC development with some slight variations. {ECO:0000269|PubMed:12832080}.
CC -!- SIMILARITY: Belongs to the expansin family. Expansin A subfamily.
CC {ECO:0000305}.
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DR EMBL; AAFI02000014; EAL69290.1; -; Genomic_DNA.
DR RefSeq; XP_643217.1; XM_638125.1.
DR AlphaFoldDB; Q7KWS2; -.
DR SMR; Q7KWS2; -.
DR STRING; 44689.DDB0231632; -.
DR PaxDb; Q7KWS2; -.
DR EnsemblProtists; EAL69290; EAL69290; DDB_G0276287.
DR GeneID; 8620421; -.
DR KEGG; ddi:DDB_G0276287; -.
DR dictyBase; DDB_G0276287; expl3.
DR eggNOG; ENOG502S9SU; Eukaryota.
DR HOGENOM; CLU_071727_0_0_1; -.
DR InParanoid; Q7KWS2; -.
DR OMA; GECKGAN; -.
DR PhylomeDB; Q7KWS2; -.
DR PRO; PR:Q7KWS2; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030248; F:cellulose binding; IDA:dictyBase.
DR GO; GO:0031153; P:slug development involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0036360; P:sorocarp stalk morphogenesis; IMP:dictyBase.
DR Gene3D; 2.40.40.10; -; 1.
DR Gene3D; 2.60.40.760; -; 1.
DR InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR InterPro; IPR036749; Expansin_CBD_sf.
DR InterPro; IPR009009; RlpA-like_DPBB.
DR InterPro; IPR036908; RlpA-like_sf.
DR Pfam; PF03330; DPBB_1; 1.
DR SMART; SM00837; DPBB_1; 1.
DR SUPFAM; SSF49590; SSF49590; 1.
DR SUPFAM; SSF50685; SSF50685; 1.
DR PROSITE; PS50842; EXPANSIN_EG45; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..335
FT /note="Expansin-like protein 3"
FT /id="PRO_0000368217"
FT TOPO_DOM 21..314
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 43..143
FT /note="Expansin-like EG45"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT REGION 247..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..76
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DISULFID 79..138
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
SQ SEQUENCE 335 AA; 37104 MW; CB255F9CF26FC037 CRC64;
MKFNTIFLVL SIVKFILISA QSCPFSQSII NGASATFYTA IDAGNCGFEK LNGPLGPGNY
MIAALGSKLY QNGAQCGQCF KISNSKNASV TVMATDSCHD AGYCQRDNHF DLSPAAFSIL
GPQSQGVLDG LSYVKVPCEV SGNVKIMMKD GSNDFWTSFF VFNSKVIIKQ VSIKLSNSNQ
FVPLSQTTYN YWPTSITGGQ FHVRIESIGG EFIYVTIPKV ESRKVYETSG QFSTSCSNLN
ENNPINYKPQ TFNSQQTSNN QNSNTQTPTK QPSPNSQNFI PSYCQQYIQK PNYIFAKESK
EMLVLNENEN IESNSLKLLP NFLLLILIIL LNINF
