ID   EXRBN_MYCTO             Reviewed;         168 AA.
AC   P9WJ72; F2GJZ2; I6Y8M5; L7N5T0; O53513; Q7D7E6;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=3'-5' exoribonuclease MT2234.1 {ECO:0000255|HAMAP-Rule:MF_00977};
DE            EC=3.1.13.- {ECO:0000255|HAMAP-Rule:MF_00977};
GN   OrderedLocusNames=MT2234.1;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Exonuclease that cleaves single-stranded 3' overhangs of
CC       double-stranded RNA. {ECO:0000255|HAMAP-Rule:MF_00977}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00977};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00977};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00977}.
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DR   EMBL; AE000516; AAK46520.1; -; Genomic_DNA.
DR   PIR; E70936; E70936.
DR   RefSeq; WP_003411331.1; NZ_KK341227.1.
DR   PDB; 4OKE; X-ray; 1.70 A; A/B=2-168.
DR   PDB; 4OKJ; X-ray; 2.10 A; A/B=2-168.
DR   PDB; 4OKK; X-ray; 2.21 A; A/B=2-168.
DR   PDBsum; 4OKE; -.
DR   PDBsum; 4OKJ; -.
DR   PDBsum; 4OKK; -.
DR   AlphaFoldDB; P9WJ72; -.
DR   SMR; P9WJ72; -.
DR   EnsemblBacteria; AAK46520; AAK46520; MT2234.1.
DR   GeneID; 45426155; -.
DR   KEGG; mtc:MT2234.1; -.
DR   PATRIC; fig|83331.31.peg.2410; -.
DR   HOGENOM; CLU_114979_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0004532; F:exoribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00977; 3_5_Exoribonuc_actinobact; 1.
DR   InterPro; IPR030853; 3_5_Exoribonuc_actinobac.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR033390; Rv2179c-like.
DR   Pfam; PF16473; DUF5051; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease.
FT   CHAIN           1..168
FT                   /note="3'-5' exoribonuclease MT2234.1"
FT                   /id="PRO_0000427880"
FT   REGION          6..9
FT                   /note="RNA binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00977"
FT   BINDING         6
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00977"
FT   STRAND          2..11
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   STRAND          16..25
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   STRAND          30..38
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           40..42
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           45..50
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           52..54
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           66..76
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   TURN            77..80
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   STRAND          81..83
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   STRAND          86..91
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           93..100
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           101..103
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           106..108
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           120..126
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   TURN            137..140
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   HELIX           142..156
FT                   /evidence="ECO:0007829|PDB:4OKE"
FT   TURN            157..159
FT                   /evidence="ECO:0007829|PDB:4OKE"
SQ   SEQUENCE   168 AA;  19520 MW;  D6CE7EF4D2FB7FA1 CRC64;
     MRYFYDTEFI EDGHTIELIS IGVVAEDGRE YYAVSTEFDP ERAGSWVRTH VLPKLPPPAS
     QLWRSRQQIR LDLEEFLRID GTDSIELWAW VGAYDHVALC QLWGPMTALP PTVPRFTREL
     RQLWEDRGCP RMPPRPRDVH DALVDARDQL RRFRLITSTD DAGRGAAR