ID   EXSA_BACCE              Reviewed;         643 AA.
AC   Q6B4J5;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Spore coat assembly protein ExsA;
GN   Name=exsA;
OS   Bacillus cereus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1396;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 10876 / DSM 9378 / NRRL B-569;
RX   PubMed=15901704; DOI=10.1128/jb.187.11.3800-3806.2005;
RA   Bailey-Smith K., Todd S.J., Southworth T.W., Proctor J., Moir A.;
RT   "The ExsA protein of Bacillus cereus is required for assembly of coat and
RT   exosporium onto the spore surface.";
RL   J. Bacteriol. 187:3800-3806(2005).
CC   -!- FUNCTION: Required for the normal assembly and anchoring of both the
CC       spore coat and the exosporium layers. {ECO:0000269|PubMed:15901704}.
CC   -!- INDUCTION: Expressed in the mother cell during sporulation.
CC   -!- DISRUPTION PHENOTYPE: Spores are extremely permeable to lysozyme and
CC       are blocked in late stages of germination.
CC       {ECO:0000269|PubMed:15901704}.
CC   -!- SIMILARITY: To B.subtilis SafA. {ECO:0000305}.
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DR   EMBL; AY682718; AAT84585.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6B4J5; -.
DR   STRING; 1396.DJ87_440; -.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   CDD; cd00118; LysM; 1.
DR   Gene3D; 3.10.350.10; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR018392; LysM_dom.
DR   InterPro; IPR036779; LysM_dom_sf.
DR   InterPro; IPR014248; Spore_coat_assembly_SafA.
DR   Pfam; PF01476; LysM; 1.
DR   SMART; SM00257; LysM; 1.
DR   SUPFAM; SSF54106; SSF54106; 1.
DR   TIGRFAMs; TIGR02899; spore_safA; 1.
DR   PROSITE; PS51782; LYSM; 1.
PE   2: Evidence at transcript level;
KW   Repeat; Sporulation.
FT   CHAIN           1..643
FT                   /note="Spore coat assembly protein ExsA"
FT                   /id="PRO_0000248939"
FT   DOMAIN          2..47
FT                   /note="LysM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01118"
FT   REPEAT          113..120
FT                   /note="1-1"
FT   REPEAT          121..128
FT                   /note="1-2"
FT   REPEAT          129..136
FT                   /note="1-3"
FT   REPEAT          137..144
FT                   /note="1-4"
FT   REPEAT          238..250
FT                   /note="2-1"
FT   REPEAT          251..263
FT                   /note="2-2"
FT   REPEAT          264..276
FT                   /note="2-3"
FT   REPEAT          277..289
FT                   /note="2-4"
FT   REPEAT          290..302
FT                   /note="2-5"
FT   REPEAT          303..315
FT                   /note="2-6"
FT   REPEAT          316..328
FT                   /note="2-7"
FT   REPEAT          354..365
FT                   /note="3-1"
FT   REPEAT          366..377
FT                   /note="3-2"
FT   REPEAT          378..389
FT                   /note="3-3"
FT   REPEAT          390..401
FT                   /note="3-4"
FT   REPEAT          402..413
FT                   /note="3-5"
FT   REPEAT          414..425
FT                   /note="3-6"
FT   REPEAT          426..437
FT                   /note="3-7"
FT   REPEAT          438..449
FT                   /note="3-8"
FT   REPEAT          450..461
FT                   /note="3-9"
FT   REPEAT          462..473
FT                   /note="3-10"
FT   REPEAT          474..485
FT                   /note="3-11"
FT   REPEAT          486..497
FT                   /note="3-12"
FT   REPEAT          498..509
FT                   /note="3-13"
FT   REPEAT          510..521
FT                   /note="3-14"
FT   REGION          113..144
FT                   /note="4 X 8 AA tandem repeats of Q-[IV]-K-P-Q-K-E-[MV]"
FT   REGION          115..160
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          238..328
FT                   /note="7 X 13 AA tandem repeats of P-Q-V-K-K-E-N-V-G-N-I-V-
FT                   S"
FT   REGION          354..521
FT                   /note="14 X 12 AA tandem repeats of I-M-D-N-N-Q-[MP]-P-N-
FT                   [IM]-M-P"
FT   REGION          585..643
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        116..159
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        603..617
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   643 AA;  72839 MW;  51BB9AC63021CFD9 CRC64;
     MKIHIVQKGD TLWKIAKKYG VDFDTLKKTN TQLSNPDLIM PGMKIKVPSK SVHMKQQAGA
     GSAPPKQYVK EVQQKEFAAT PTPLGIEDEE EVTYQSAPIT QQPAMQQTQK EVQIKPQKEM
     QVKPQKEVQV KPQKEMQVKP QKEVQKEQPI QKEKPVEKPS VIQKPPVIEK QKPAEKENTK
     FSVNVLPQPP QPPIKPKKEY KISDVIKKGS ELIAPQISKM KPNNIISPQT KKNNIISPQV
     KKENVGNIVS PQVKKENVGN IVSPQVKKEN VGNIVSPQVK KENVGNIVSP QVKKENVGNI
     VSPQVKKENV GNIVSPQVKK ENVGNIVSPN VSKENVVIPQ VIPPNIQMPN IMPIMDNNQP
     PNIMPIMDNN QPPNIMPIMD NNQMPNMMPI MDNNQMPNMM PIMDNNQMPN MMPIMDNNQM
     PNMMPIMDNN QMPNMMPIMD NNQMPNMMPI MDNNQMPNMM PIMDNNQMPN IMPIMDNNQM
     PNMMPIMDNN QMPNIMPIMD NNQMPNMMPI MDNNQPPNMM PYQMPYQQPM MPPNPYYQQP
     NPYQMPYQQG APFGPQHTSM PNQNMMPMDN NMPPLVQGEE DCGCGGESRL YSPQPGGPQY
     ANPLYYQPTQ SAYAPQPGTM YYQPDPPNVF GEPVSEEEDE EEV