EXSB_PSEAE
ID EXSB_PSEAE Reviewed; 137 AA.
AC P26994;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Type 3 secretion system pilotin {ECO:0000305};
DE AltName: Full=Exoenzyme S synthesis protein B;
DE AltName: Full=Pilotin {ECO:0000303|PubMed:25690097};
DE Flags: Precursor;
GN Name=exsB {ECO:0000303|PubMed:1655713}; OrderedLocusNames=PA1712;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=1655713; DOI=10.1128/jb.173.20.6460-6468.1991;
RA Frank D.W., Iglewski B.H.;
RT "Cloning and sequence analysis of a trans-regulatory locus required for
RT exoenzyme S synthesis in Pseudomonas aeruginosa.";
RL J. Bacteriol. 173:6460-6468(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25690097; DOI=10.1128/iai.00048-15;
RA Perdu C., Huber P., Bouillot S., Blocker A., Elsen S., Attree I.,
RA Faudry E.;
RT "ExsB is required for correct assembly of the Pseudomonas aeruginosa type
RT III secretion apparatus in the bacterial membrane and full virulence in
RT vivo.";
RL Infect. Immun. 83:1789-1798(2015).
RN [4] {ECO:0007744|PDB:2YJL}
RP X-RAY CRYSTALLOGRAPHY (1.81 ANGSTROMS) OF 13-137, SUBCELLULAR LOCATION, AND
RP DOMAIN.
RX PubMed=21839744; DOI=10.1016/j.jmb.2011.07.043;
RA Izore T., Perdu C., Job V., Attree I., Faudry E., Dessen A.;
RT "Structural characterization and membrane localization of ExsB from the
RT type III secretion system (T3SS) of Pseudomonas aeruginosa.";
RL J. Mol. Biol. 413:236-246(2011).
CC -!- FUNCTION: Involved in the synthesis of the type III secretion system
CC (T3SS), also called injectisome, which is used to inject bacterial
CC effector proteins into eukaryotic host cells (PubMed:25690097). Pilot
CC protein that is required for the proper localization of the secretin
CC PscC in the outer membrane (PubMed:25690097). Necessary for full in
CC vivo virulence (PubMed:25690097). {ECO:0000269|PubMed:25690097}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000269|PubMed:21839744}. Note=Could be a lipoprotein.
CC {ECO:0000305|PubMed:21839744}.
CC -!- DOMAIN: Displays a compact beta-sandwich fold with interdependent beta-
CC sheets. Possesses a large patch of basic residues that could play a
CC role in membrane recognition. {ECO:0000269|PubMed:21839744}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene causes a diminution of T3SS
CC needles, a decrease in secretion of T3SS proteins and affects the
CC regulatory gene operon. It reduces T3SS activity toward eukaryotic
CC cells. Mutant shows reduced virulence in a mouse pneumonia model.
CC {ECO:0000269|PubMed:25690097}.
CC -!- SIMILARITY: Belongs to the ExsB/YscW family. {ECO:0000305}.
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DR EMBL; M64975; AAA25815.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG05101.1; -; Genomic_DNA.
DR PIR; B41047; B41047.
DR PIR; E83433; E83433.
DR RefSeq; NP_250403.1; NC_002516.2.
DR RefSeq; WP_003100765.1; NZ_QZGE01000003.1.
DR PDB; 2YJL; X-ray; 1.81 A; A/B/C=13-137.
DR PDBsum; 2YJL; -.
DR AlphaFoldDB; P26994; -.
DR SMR; P26994; -.
DR STRING; 287.DR97_175; -.
DR PaxDb; P26994; -.
DR DNASU; 878557; -.
DR EnsemblBacteria; AAG05101; AAG05101; PA1712.
DR GeneID; 878557; -.
DR KEGG; pae:PA1712; -.
DR PATRIC; fig|208964.12.peg.1774; -.
DR PseudoCAP; PA1712; -.
DR HOGENOM; CLU_1833466_0_0_6; -.
DR OMA; LPCYPEC; -.
DR BioCyc; PAER208964:G1FZ6-1743-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR039366; Pilotin.
DR Pfam; PF09619; YscW; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Membrane; Reference proteome; Signal;
KW Virulence.
FT SIGNAL 1..14
FT /evidence="ECO:0000255"
FT CHAIN 15..137
FT /note="Type 3 secretion system pilotin"
FT /evidence="ECO:0000255"
FT /id="PRO_0000087141"
FT CONFLICT 62
FT /note="R -> W (in Ref. 1; AAA25815)"
FT /evidence="ECO:0000305"
FT STRAND 22..32
FT /evidence="ECO:0007829|PDB:2YJL"
FT STRAND 39..49
FT /evidence="ECO:0007829|PDB:2YJL"
FT STRAND 52..66
FT /evidence="ECO:0007829|PDB:2YJL"
FT STRAND 68..75
FT /evidence="ECO:0007829|PDB:2YJL"
FT HELIX 76..79
FT /evidence="ECO:0007829|PDB:2YJL"
FT STRAND 84..92
FT /evidence="ECO:0007829|PDB:2YJL"
FT STRAND 100..106
FT /evidence="ECO:0007829|PDB:2YJL"
FT STRAND 110..115
FT /evidence="ECO:0007829|PDB:2YJL"
FT TURN 120..123
FT /evidence="ECO:0007829|PDB:2YJL"
SQ SEQUENCE 137 AA; 14996 MW; F7367E7AB301C4EA CRC64;
MLLPLALLLG GCVSQPAPMS PKVTVGGSVG GVSLQARQAQ LRLRLYAVVQ GRMQTIAERR
YRVSGLPLRY AFDLEVDRLE GEALYLRTEL SWVGVAAVQA SAWQQVAAGV DERVRLVRRD
CFPNCTAARP EERSGND
