EXTN2_ARATH
ID EXTN2_ARATH Reviewed; 743 AA.
AC Q9M1G9; F4JCZ2; Q9CAZ9;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Extensin-2 {ECO:0000303|PubMed:11475326, ECO:0000303|PubMed:20395450};
DE Short=AtExt2 {ECO:0000303|PubMed:11475326, ECO:0000303|PubMed:20395450};
DE AltName: Full=Cell wall hydroxyproline-rich glycoprotein 1;
DE Short=HRGP1;
DE Flags: Precursor;
GN Name=EXT2 {ECO:0000303|PubMed:11475326, ECO:0000303|PubMed:20395450};
GN Synonyms=HRGP1;
GN OrderedLocusNames=At3g54590 {ECO:0000312|Araport:AT3G54590};
GN ORFNames=T14E10.160 {ECO:0000312|EMBL:CAB77579.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11475326; DOI=10.1093/dnares/8.3.115;
RA Yoshiba Y., Aoki C., Iuchi S., Nanjo T., Seki M., Sekiguchi F.,
RA Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Characterization of four extensin genes in Arabidopsis thaliana by
RT differential gene expression under stress and non-stress conditions.";
RL DNA Res. 8:115-122(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20395450; DOI=10.1104/pp.110.156554;
RA Showalter A.M., Keppler B., Lichtenberg J., Gu D., Welch L.R.;
RT "A bioinformatics approach to the identification, classification, and
RT analysis of hydroxyproline-rich glycoproteins.";
RL Plant Physiol. 153:485-513(2010).
CC -!- FUNCTION: Structural component which strengthens the primary cell wall.
CC -!- SUBCELLULAR LOCATION: Secreted, primary cell wall.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the roots.
CC -!- DEVELOPMENTAL STAGE: Early expressed in the whole plant, but was
CC restricted to lower stems, flower buds and roots in the mature plant (6
CC weeks old).
CC -!- INDUCTION: By wounding and water stress; in response to plant hormones
CC 2,4-D, BAP treatment; in response to L-Pro treatment. Repressed by salt
CC stress.
CC -!- PTM: Extensins contain a characteristic repeat of the pentapeptide Ser-
CC Pro(4). The proline residues are hydroxylated and then O-glycosylated
CC (arabinosylation).
CC -!- PTM: Synthetised as soluble proteins which become insolubilised in the
CC cell wall through the intermolecular cross-linking of Tyr on adjacent
CC monomers. Isodityrosine (IDT) stabilizes and makes rigid the part of
CC the polypeptide where IDT functional sites are present.
CC -!- SIMILARITY: Belongs to the extensin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AEE79253.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAB21544.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB21544.1; Type=Miscellaneous discrepancy; Note=Incomplete cDNA clone.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB022782; BAB21544.1; ALT_SEQ; mRNA.
DR EMBL; AL138656; CAB77579.2; -; Genomic_DNA.
DR EMBL; CP002686; AEE79253.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; ANM63926.1; -; Genomic_DNA.
DR PIR; T47618; T47618.
DR RefSeq; NP_001325986.1; NM_001339685.1.
DR RefSeq; NP_191022.2; NM_115316.3.
DR AlphaFoldDB; Q9M1G9; -.
DR BioGRID; 9940; 1.
DR STRING; 3702.AT3G54590.1; -.
DR PaxDb; Q9M1G9; -.
DR EnsemblPlants; AT3G54590.3; AT3G54590.3; AT3G54590.
DR GeneID; 824624; -.
DR Gramene; AT3G54590.3; AT3G54590.3; AT3G54590.
DR KEGG; ath:AT3G54590; -.
DR Araport; AT3G54590; -.
DR TAIR; locus:2096976; AT3G54590.
DR eggNOG; ENOG502RHFU; Eukaryota.
DR HOGENOM; CLU_439011_0_0_1; -.
DR OMA; ATNDRSW; -.
DR PRO; PR:Q9M1G9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1G9; baseline and differential.
DR Genevisible; Q9M1G9; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009530; C:primary cell wall; IEA:UniProtKB-SubCell.
DR GO; GO:0005199; F:structural constituent of cell wall; IEA:InterPro.
DR GO; GO:0009664; P:plant-type cell wall organization; IEA:InterPro.
DR InterPro; IPR006706; Extensin_dom.
DR Pfam; PF04554; Extensin_2; 23.
PE 2: Evidence at transcript level;
KW Cell wall; Cell wall biogenesis/degradation; Glycoprotein; Hydroxylation;
KW Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..743
FT /note="Extensin-2"
FT /id="PRO_0000008726"
FT REPEAT 70..78
FT /note="1-1"
FT REPEAT 79..94
FT /note="2-1"
FT REPEAT 95..103
FT /note="1-2"
FT REPEAT 104..119
FT /note="2-2"
FT REPEAT 120..128
FT /note="1-3"
FT REPEAT 129..144
FT /note="2-3"
FT REPEAT 145..153
FT /note="1-4"
FT REPEAT 154..169
FT /note="2-4"
FT REPEAT 170..178
FT /note="1-5"
FT REPEAT 179..194
FT /note="2-5"
FT REPEAT 195..203
FT /note="1-6"
FT REPEAT 204..219
FT /note="2-6"
FT REPEAT 220..228
FT /note="1-7"
FT REPEAT 229..244
FT /note="2-7"
FT REPEAT 245..253
FT /note="1-8"
FT REPEAT 254..269
FT /note="2-8"
FT REPEAT 270..278
FT /note="1-9"
FT REPEAT 279..294
FT /note="2-9"
FT REPEAT 295..303
FT /note="1-10"
FT REPEAT 304..319
FT /note="2-10"
FT REPEAT 320..328
FT /note="1-11"
FT REPEAT 329..344
FT /note="2-11"
FT REPEAT 345..353
FT /note="1-12"
FT REPEAT 354..369
FT /note="2-12"
FT REPEAT 370..378
FT /note="1-13"
FT REPEAT 379..394
FT /note="2-13"
FT REPEAT 395..403
FT /note="1-14"
FT REPEAT 404..419
FT /note="2-14"
FT REPEAT 420..428
FT /note="1-15"
FT REPEAT 429..444
FT /note="2-15"
FT REPEAT 445..453
FT /note="1-16"
FT REPEAT 454..469
FT /note="2-16"
FT REPEAT 470..478
FT /note="1-17"
FT REPEAT 479..494
FT /note="2-17"
FT REPEAT 495..503
FT /note="1-18"
FT REPEAT 504..519
FT /note="2-18"
FT REPEAT 520..528
FT /note="1-19"
FT REPEAT 529..544
FT /note="2-19"
FT REPEAT 545..553
FT /note="1-20"
FT REPEAT 554..569
FT /note="2-20"
FT REPEAT 570..578
FT /note="1-21"
FT REPEAT 579..594
FT /note="2-21"
FT REPEAT 595..603
FT /note="1-22"
FT REPEAT 604..619
FT /note="2-22"
FT REPEAT 620..628
FT /note="1-23"
FT REPEAT 629..644
FT /note="2-23"
FT REPEAT 645..660
FT /note="2-24"
FT REGION 46..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 70..628
FT /note="23 X 9 AA repeats of S-P-P-P-P-Y-V-Y-[SN]"
FT REGION 79..660
FT /note="24 X 16 AA repeats of S-P-P-P-P-[YT]-Y-S-P-S-P-K-V-
FT [DEYH]-Y-K"
FT REGION 715..743
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..93
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 743 AA; 83015 MW; 251E231903D9EEB4 CRC64;
MGPSAHLISA LGVIIMATMV AAYEPETYAS PPPLYSSPLP EVEYKTPPLP YVDSSPPPTY
TPAPEVEYKS PPPPYVYSSP PPPTYSPSPK VDYKSPPPPY VYSSPPPPYY SPSPKVDYKS
PPPPYVYNSP PPPYYSPSPK VDYKSPPPPY VYSSPPPPYY SPSPKVEYKS PPPPYVYSSP
PPPYYSPSPK VDYKSPPPPY VYSSPPPPYY SPSPKVEYKS PPPPYVYSSP PPPYYSPSPK
VDYKSPPPPY VYSSPPPPYY SPSPKVDYKS PPPPYVYSSP PPPYYSPSPK VDYKSPPPPY
VYSSPPPPYY SPSPKVDYKS PPPPYVYSSP PPPYYSPSPK VDYKSPPPPY VYSSPPPPTY
SPSPKVDYKS PPPPYVYSSP PPPYYSPSPK VEYKSPPPPY VYSSPPPPTY SPSPKVYYKS
PPPPYVYSSP PPPYYSPSPK VYYKSPPPPY VYSSPPPPYY SPSPKVYYKS PPPPYVYSSP
PPPYYSPSPK VYYKSPPPPY VYSSPPPPYY SPSPKVYYKS PPPPYVYSSP PPPYYSPSPK
VHYKSPPPPY VYSSPPPPYY SPSPKVHYKS PPPPYVYNSP PPPYYSPSPK VYYKSPPPPY
VYSSPPPPYY SPSPKVYYKS PPPPYVYSSP PPPYYSPSPK VYYKSPPPPY YSPSPKVYYK
SPPHPHVCVC PPPPPCYSPS PKVVYKSPPP PYVYNSPPPP YYSPSPKVYY KSPPPPSYYS
PSPKVEYKSP PPPSYSPSPK TEY
