ID   EXUT_DICCH              Reviewed;         345 AA.
AC   P94774;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Galacturonate transporter {ECO:0000305};
DE   Flags: Precursor;
GN   Name=exuT {ECO:0000303|PubMed:8320243};
OS   Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Dickeya.
OX   NCBI_TaxID=556;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=EC16;
RX   DOI=10.1111/j.1574-6968.1994.tb06810.x;
RA   San Francisco M.J., Freeman T.L.;
RT   "Cloning of a galacturonic acid uptake gene from Erwinia chrysanthemi
RT   EC16.";
RL   FEMS Microbiol. Lett. 118:101-106(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, INDUCTION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=EC16;
RX   PubMed=9530868; DOI=10.1094/MPMI.1998.11.4.270;
RA   Haseloff B.J., Freeman T.L., Valmeekam V., Melkus M.W., Oner F.,
RA   Valachovic M.S., San Francisco M.J.;
RT   "The exuT gene of Erwinia chrysanthemi EC16: nucleotide sequence,
RT   expression, localization, and relevance of the gene product.";
RL   Mol. Plant Microbe Interact. 11:270-276(1998).
RN   [3]
RP   FUNCTION, AND ACTIVITY REGULATION.
RC   STRAIN=EC16;
RX   PubMed=8320243; DOI=10.1128/jb.175.13.4263-4265.1993;
RA   San Francisco M.J., Keenan R.W.;
RT   "Uptake of galacturonic acid in Erwinia chrysanthemi EC16.";
RL   J. Bacteriol. 175:4263-4265(1993).
RN   [4]
RP   INDUCTION.
RX   PubMed=11386378; DOI=10.1094/mpmi.2001.14.6.816;
RA   Valmeekam V., Loh Y.L., San Francisco M.J.;
RT   "Control of exuT activity for galacturonate transport by the negative
RT   regulator ExuR in Erwinia chrysanthemi EC16.";
RL   Mol. Plant Microbe Interact. 14:816-820(2001).
CC   -!- FUNCTION: Transport of D-galacturonate (PubMed:8320243, Ref.1). Cannot
CC       transport the dimer digalacturonic acid (PubMed:8320243). Uptake is an
CC       active process (PubMed:8320243). {ECO:0000269|PubMed:8320243,
CC       ECO:0000269|Ref.1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=aldehydo-D-galacturonate(out) + H(+)(out) = aldehydo-D-
CC         galacturonate(in) + H(+)(in); Xref=Rhea:RHEA:29295,
CC         ChEBI:CHEBI:12952, ChEBI:CHEBI:15378;
CC         Evidence={ECO:0000305|PubMed:8320243};
CC   -!- ACTIVITY REGULATION: Inhibited by cyanide and 2,4-dinitrophenol, but
CC       not by arsenate. {ECO:0000269|PubMed:8320243}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:9530868};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Induced in the presence of D-galacturonate (Ref.1,
CC       PubMed:9530868, PubMed:11386378). Expression is regulated by ExuR
CC       (PubMed:11386378). Maximal transcription activity is observed within 8
CC       hours of bacterial inoculation into potato tubers, well before any
CC       visible symptoms of disease are detected (PubMed:11386378).
CC       {ECO:0000269|PubMed:11386378, ECO:0000269|PubMed:9530868,
CC       ECO:0000269|Ref.1}.
CC   -!- DISRUPTION PHENOTYPE: Mutation of the gene causes a reduction in growth
CC       on D-galacturonate and maceration of potato tuber tissue.
CC       {ECO:0000269|PubMed:9530868}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Phthalate
CC       permease family. {ECO:0000305}.
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DR   EMBL; U86689; AAB70881.1; -; Genomic_DNA.
DR   TCDB; 2.A.1.14.41; the major facilitator superfamily (mfs).
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Signal; Transmembrane;
KW   Transmembrane helix; Transport.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..345
FT                   /note="Galacturonate transporter"
FT                   /id="PRO_0000456285"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..96
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        237..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        265..285
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   345 AA;  37870 MW;  2B25B66CC2BE3BFD CRC64;
     MFKIKGLRWY MIGLVTIGTV LGYLTRNAIA AAAPTLQEQL HISTQQYSYI IAAYSACYTI
     MQPVAGYVLD VLGTKVGYAM FAILWALFCA GTALANSWGG LAVARGAVGM AEAAMIPAGL
     KASSEWFPAK ERSVAVGYFN VGSSIGGMLA PPLVVWAIMA HSWQMAFLIT GALSLVWALC
     WLYFYKHPKD QKKLSTEERE YILSGQEAQH QAGNAKRMSA WQILRNRQFW GIALPRFLAE
     PAWGTFNAWI PLFMFKAYGF NLKEIAMFAW MPMLFADLGC ILGGYMPMLF QKYFKVNLIV
     SRKLVVTLGA LLMIGPGTIG LFTSPYVAIA CCASAALPTS PCPVR