EXUT_ECO57
ID EXUT_ECO57 Reviewed; 432 AA.
AC P0AA79; P42609;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-JUN-2014, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Hexuronate transporter {ECO:0000250|UniProtKB:P0AA78};
DE AltName: Full=Aldohexuronate transport system {ECO:0000250|UniProtKB:P0AA78};
DE Flags: Precursor;
GN Name=exuT; OrderedLocusNames=Z4446, ECs3975;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Transport of aldohexuronates such as D-glucuronate and D-
CC galacturonate. {ECO:0000250|UniProtKB:P0AA78}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=aldehydo-D-glucuronate(in) + H(+)(in) = aldehydo-D-
CC glucuronate(out) + H(+)(out); Xref=Rhea:RHEA:28955,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:142686;
CC Evidence={ECO:0000250|UniProtKB:P0AA78};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=aldehydo-D-galacturonate(out) + H(+)(out) = aldehydo-D-
CC galacturonate(in) + H(+)(in); Xref=Rhea:RHEA:29295,
CC ChEBI:CHEBI:12952, ChEBI:CHEBI:15378;
CC Evidence={ECO:0000250|UniProtKB:P0AA78};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0AA78}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Phthalate
CC permease family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG58226.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB37398.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE005174; AAG58226.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000007; BAB37398.1; ALT_INIT; Genomic_DNA.
DR PIR; F85970; F85970.
DR PIR; G91125; G91125.
DR RefSeq; NP_312002.1; NC_002695.1.
DR RefSeq; WP_001226465.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AA79; -.
DR SMR; P0AA79; -.
DR STRING; 155864.EDL933_4315; -.
DR EnsemblBacteria; AAG58226; AAG58226; Z4446.
DR EnsemblBacteria; BAB37398; BAB37398; ECs_3975.
DR GeneID; 58389404; -.
DR GeneID; 916191; -.
DR KEGG; ece:Z4446; -.
DR KEGG; ecs:ECs_3975; -.
DR PATRIC; fig|386585.9.peg.4149; -.
DR eggNOG; COG2271; Bacteria.
DR HOGENOM; CLU_001265_5_1_6; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..432
FT /note="Hexuronate transporter"
FT /id="PRO_0000121380"
FT TOPO_DOM 33..48
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..75
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..99
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..164
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..236
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..264
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..293
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 315..317
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 391
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 413..432
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AA78"
SQ SEQUENCE 432 AA; 46892 MW; 9C3B2422F2B28ED8 CRC64;
MRKIKGLRWY MIALVTLGTV LGYLTRNTVA AAAPTLMEEL NISTQQYSYI IAAYSAAYTV
MQPVAGYVLD VLGTKIGYAM FAVLWAVFCG ATALAGSWGG LAVARGAVGA AEAAMIPAGL
KASSEWFPAK ERSIAVGYFN VGSSIGAMIA PPLVVWAIVM HSWQMAFIIS GALSFIWAMA
WLIFYKHPRD QKHLTDEERD YIINGQEAQH QVSTAKKMSV GQILRNRQFW GIALPRFLAE
PAWGTFNAWI PLFMFKVYGF NLKEIAMFAW MPMLFADLGC ILGGYLPPLF QRWFGVNLIV
SRKMVVTLGA VLMIGPGMIG LFTNPYVAIM LLCIGGFAHQ ALSGALITLS SDVFGRNEVA
TANGLTGMSA WLASTLFALV VGALADTIGF SPLFAVLAVF DLLGALVIWT VLQNKPAIEV
AQETHNDPAP QH
