AHL12_ARATH
ID AHL12_ARATH Reviewed; 361 AA.
AC Q8LPN5; Q8VYP4; Q9SH33;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=AT-hook motif nuclear-localized protein 12 {ECO:0000312|EMBL:FAA00283.1};
GN Name=AHL12 {ECO:0000303|PubMed:15604740};
GN OrderedLocusNames=At1g63480 {ECO:0000312|Araport:AT1G63480};
GN ORFNames=F2K11.15 {ECO:0000312|EMBL:AAF19697.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15604740; DOI=10.1007/s11103-004-3249-5;
RA Fujimoto S., Matsunaga S., Yonemura M., Uchiyama S., Azuma T., Fukui K.;
RT "Identification of a novel plant MAR DNA binding protein localized on
RT chromosomal surfaces.";
RL Plant Mol. Biol. 56:225-239(2004).
RN [5]
RP GENE FAMILY, SUBUNIT, INTERACTION WITH AHL27; AHL29 AND ATAF2/NAC081, AND
RP DOMAIN PPC.
RX PubMed=24218605; DOI=10.1073/pnas.1219277110;
RA Zhao J., Favero D.S., Peng H., Neff M.M.;
RT "Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by
RT interacting with each other via the PPC/DUF296 domain.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E4688-E4697(2013).
CC -!- FUNCTION: Transcription factor that specifically binds AT-rich DNA
CC sequences related to the nuclear matrix attachment regions (MARs).
CC {ECO:0000250|UniProtKB:Q8VYJ2}.
CC -!- SUBUNIT: Homodimer. Interacts with AHL27, AHL29 and ATAF2/NAC081.
CC {ECO:0000269|PubMed:24218605}.
CC -!- INTERACTION:
CC Q8LPN5; Q17TI5: BRX; NbExp=3; IntAct=EBI-4428689, EBI-4426649;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8VYJ2}.
CC -!- DOMAIN: The PPC domain mediates interactions between AHL proteins.
CC {ECO:0000269|PubMed:24218605}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF19697.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAL49880.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC008047; AAF19697.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34104.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60201.1; -; Genomic_DNA.
DR EMBL; AY070384; AAL49880.1; ALT_INIT; mRNA.
DR EMBL; AY096576; AAM20226.1; -; mRNA.
DR EMBL; BR000348; FAA00283.1; -; mRNA.
DR RefSeq; NP_001322498.1; NM_001334099.1.
DR RefSeq; NP_001322499.1; NM_001334098.1.
DR RefSeq; NP_001322500.1; NM_001334097.1.
DR RefSeq; NP_001322501.1; NM_001334096.1.
DR RefSeq; NP_001322502.1; NM_001334095.1.
DR RefSeq; NP_001322503.1; NM_001334094.1.
DR RefSeq; NP_176537.2; NM_105027.4.
DR AlphaFoldDB; Q8LPN5; -.
DR SMR; Q8LPN5; -.
DR IntAct; Q8LPN5; 4.
DR STRING; 3702.AT1G63480.1; -.
DR iPTMnet; Q8LPN5; -.
DR PaxDb; Q8LPN5; -.
DR PRIDE; Q8LPN5; -.
DR ProteomicsDB; 244946; -.
DR EnsemblPlants; AT1G63480.1; AT1G63480.1; AT1G63480.
DR EnsemblPlants; AT1G63480.2; AT1G63480.2; AT1G63480.
DR GeneID; 842654; -.
DR Gramene; AT1G63480.1; AT1G63480.1; AT1G63480.
DR Gramene; AT1G63480.2; AT1G63480.2; AT1G63480.
DR KEGG; ath:AT1G63480; -.
DR Araport; AT1G63480; -.
DR TAIR; locus:2031306; AT1G63480.
DR eggNOG; ENOG502QUPI; Eukaryota.
DR HOGENOM; CLU_039808_0_2_1; -.
DR InParanoid; Q8LPN5; -.
DR OrthoDB; 937709at2759; -.
DR PhylomeDB; Q8LPN5; -.
DR PRO; PR:Q8LPN5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LPN5; baseline and differential.
DR Genevisible; Q8LPN5; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; IEA:InterPro.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR CDD; cd11378; DUF296; 1.
DR InterPro; IPR039605; AHL.
DR InterPro; IPR017956; AT_hook_DNA-bd_motif.
DR InterPro; IPR005175; PPC_dom.
DR PANTHER; PTHR31500; PTHR31500; 1.
DR Pfam; PF03479; PCC; 1.
DR PRINTS; PR00929; ATHOOK.
DR PROSITE; PS51742; PPC; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..361
FT /note="AT-hook motif nuclear-localized protein 12"
FT /id="PRO_0000432030"
FT DOMAIN 154..297
FT /note="PPC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01078"
FT DNA_BIND 99..111
FT /note="A.T hook 1"
FT /evidence="ECO:0000255"
FT DNA_BIND 130..142
FT /note="A.T hook 2"
FT /evidence="ECO:0000255"
FT REGION 29..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 286..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 99..107
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000305"
FT COMPBIAS 95..113
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..336
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 361 AA; 38312 MW; ED20E337F8C1BCD0 CRC64;
MDGREAMAFP GSHSQYYLQR GAFTNLAPSQ VASGLHAPPP HTGLRPMSNP NIHHPQANNP
GPPFSDFGHT IHMGVVSSAS DADVQPPPPP PPPEEPMVKR KRGRPRKYGE PMVSNKSRDS
SPMSDPNEPK RARGRPPGTG RKQRLANLGE WMNTSAGLAF APHVISIGAG EDIAAKVLSF
SQQRPRALCI MSGTGTISSV TLCKPGSTDR HLTYEGPFEI ISFGGSYLVN EEGGSRSRTG
GLSVSLSRPD GSIIAGGVDM LIAANLVQVV ACSFVYGARA KTHNNNNKTI RQEKEPNEED
NNSEMETTPG SAAEPAASAG QQTPQNFSSQ GIRGWPGSGS GSGRSLDICR NPLTDFDLTR
G
