EZ1_ORYSI
ID EZ1_ORYSI Reviewed; 895 AA.
AC Q84UI6;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Histone-lysine N-methyltransferase EZ1 {ECO:0000305};
DE Short=OsiEZ1 {ECO:0000303|PubMed:14527713};
DE EC=2.1.1.356 {ECO:0000255|PROSITE-ProRule:PRU00909};
GN Name=EZ1 {ECO:0000305}; Synonyms=SET1 {ECO:0000305};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Pusa Basmati;
RX PubMed=14527713; DOI=10.1016/s0378-1119(03)00723-6;
RA Thakur J.K., Malik M.R., Bhatt V., Reddy M.K., Sopory S.K., Tyagi A.K.,
RA Khurana J.P.;
RT "A POLYCOMB group gene of rice (Oryza sativa L. subspecies indica), OsiEZ1,
RT codes for a nuclear-localized protein expressed preferentially in young
RT seedlings and during reproductive development.";
RL Gene 314:1-13(2003).
CC -!- FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of some PcG
CC multiprotein complex, which methylates 'Lys-27' of histone H3, leading
CC to transcriptional repression of the affected target genes. PcG
CC proteins act by forming multiprotein complexes, which are required to
CC maintain the transcriptionally repressive state of homeotic genes
CC throughout development. PcG proteins are not required to initiate
CC repression, but to maintain it during later stages of development (By
CC similarity). Involved in histone methylation and transcription
CC regulation (Probable). {ECO:0000250|UniProtKB:Q10MI4,
CC ECO:0000305|PubMed:14527713}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = 3 H(+)
CC + N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:60292, Rhea:RHEA-COMP:15535, Rhea:RHEA-
CC COMP:15548, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:61961; EC=2.1.1.356;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00909};
CC -!- SUBUNIT: Component of the polycomb repressive complex 2 (PRC2), which
CC methylates 'Lys-27' residues of histone H3 (H3K27me3), leading to
CC transcriptional repression of the affected target gene.
CC {ECO:0000250|UniProtKB:Q10MI4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14527713}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, shoots, rachis, anthers
CC and embryos. {ECO:0000269|PubMed:14527713}.
CC -!- DEVELOPMENTAL STAGE: Expressed in emerging young seedlings from 3 to 4
CC days after seed imbibition. {ECO:0000269|PubMed:14527713}.
CC -!- SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase
CC superfamily. Histone-lysine methyltransferase family. EZ subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00909}.
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DR EMBL; AJ421722; CAD18871.3; -; mRNA.
DR AlphaFoldDB; Q84UI6; -.
DR SMR; Q84UI6; -.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0031519; C:PcG protein complex; IEA:InterPro.
DR GO; GO:0046976; F:histone methyltransferase activity (H3-K27 specific); IEA:UniProtKB-EC.
DR GO; GO:0006338; P:chromatin remodeling; IEA:InterPro.
DR GO; GO:0016571; P:histone methylation; TAS:UniProtKB.
DR CDD; cd00167; SANT; 1.
DR Gene3D; 2.170.270.10; -; 1.
DR InterPro; IPR026489; CXC_dom.
DR InterPro; IPR045318; EZH1/2-like.
DR InterPro; IPR025778; Hist-Lys_N-MeTrfase_plant.
DR InterPro; IPR041355; Pre-SET_CXC.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR001214; SET_dom.
DR InterPro; IPR046341; SET_dom_sf.
DR InterPro; IPR033467; Tesmin/TSO1-like_CXC.
DR PANTHER; PTHR45747; PTHR45747; 1.
DR Pfam; PF18264; preSET_CXC; 1.
DR Pfam; PF00856; SET; 1.
DR SMART; SM01114; CXC; 1.
DR SMART; SM00317; SET; 1.
DR SUPFAM; SSF82199; SSF82199; 1.
DR PROSITE; PS51633; CXC; 1.
DR PROSITE; PS51576; SAM_MT43_EZ; 1.
DR PROSITE; PS50280; SET; 1.
PE 2: Evidence at transcript level;
KW Chromatin regulator; Methyltransferase; Nucleus; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..895
FT /note="Histone-lysine N-methyltransferase EZ1"
FT /id="PRO_0000444466"
FT DOMAIN 628..732
FT /note="CXC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00970"
FT DOMAIN 747..862
FT /note="SET"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00190"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 369..425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 485..508
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 867..895
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 386..403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 873..889
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 861
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00190"
SQ SEQUENCE 895 AA; 99776 MW; BB618FCD0E414FA2 CRC64;
MASSSSKASD SSSQRPKRPD QGPSGKDAAG LVVLHGKLAQ LKRQVQSTRL AAIKERVEAN
RKALQVHTCA LFDVAAAAEV ASRGAEGGNA LSRGAAEGHC RLVGWDSASG PGERELVHVQ
EENLVAGTLV LSSSGGSGAS HRTVVQLAKL PVVDKIPPYT TWIFLDKNQR MADDQLVCRR
RIYYDPIVNE ALICSESDDD VPEPEEEKHV FTEGEDQLIW KATQDHGLSR EVLNVLCQFV
DATPSEIEER SEVLFEKYEK QSQSSYETDF QLFLGKTMDV ALDSFDNLFC RRCLVFDCRL
HGCSQNLVFP SEKQPYGHGL DENKRPCGDQ RYLRRREVYQ DTCNDDRNAC TTYNTDSRSS
SLKVSATILS ESEDSNRDED NIKSTSIVET SRSKITNSEY ADKSVTPPPG DASETENVSP
DMPLRTLGRR KISKHASKSN DHSPDKRQKI YSSPFPFAMS VLNKQSVPEI GETCPDSIES
AVDQLPSLDD PNKKISTKDM CAGSTTNTTE NTLRDNNNNL FISNKEHSIS HWSALERDLY
LKGIEIFGKN SCLIARNLLS GLKTCMEVAS YMYNNGAAMA KRPLSGKSIL GDFAEAEQGY
MEQDLVARTR ICRRKGRARK LKYTWKSAGH PTVRKRIGDG KQWYTQYNPC GCQQMCGKDC
ACVENGTCCE KYCGCSKSCK NRFRGCHCAK SQCRSRQCPC FAASRECDPD VCRNCWVSCG
DGSLGEPLAR GDGYQCGNMK LLLKQQQRIL LGKSDVAGWG AFIKNPVNRN DYLGEYTGEL
ISHREADKRG KIYDRANSSF LFDLNEQYVL DAYRKGDKLK FANHSSNPNC YAKVMLVAGD
HRVGIYAKDR IEASEELFYD YRYGPDQAPA WARRPEGSKK DEASVSHHRA HKVAR
